LOCUS       sequence-364156-       10873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p300 HAT core domain coupled to catalytically dead dCas9 for 
            localization; labeled with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
  TITLE     Transcription activation is enhanced by multivalent interactions 
            independent of phase separation.
  JOURNAL   Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017.
  PUBMED    35537448
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017."
COMMENT     Sequence Label: NLS-dCas9-HA-NLS--GFP-p300
FEATURES             Location/Qualifiers
     source          1..10873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(25..453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(135..156)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     347..366
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        480..584
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        586..777
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(609..629)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      774..907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             956..1750
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             956..1750
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1010..1029)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1620..1639
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1938..1957)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1982..2029
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2358..2946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2847..2866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3121..3424
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3425..3628
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3579..3599
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3688..3708
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3757..3766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3763..3789,3790..3792,3793..6279,6280..6282,
                     6283..7866)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3763..7866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7870..7896
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7903..7923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7981..7990
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7987..7989,7990..7992,7993..8703)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7987..7989,7990..7992,7993..8703)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8293..8312)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8640..8661
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8728..10578
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDGPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      10342..10351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10579..10605
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    10743..10864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10780..10799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10834..10853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
       61 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      121 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      181 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      241 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      301 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      361 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      421 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
      481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
      601 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      661 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      781 cctaactccg cccatcccgc ccattctccg ccccatggct gactaatttt ttttatttat
      841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      901 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
      961 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     1021 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     1081 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     1141 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     1201 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     1261 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     1321 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     1381 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     1441 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     1501 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     1561 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     1621 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     1681 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     1741 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1801 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1861 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1921 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     1981 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     2041 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     2101 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     2161 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     2221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     3061 tagttattaa tagtaatcaa ttactgggtc attagttcat agcccatata tggagttccg
     3121 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3181 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3241 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3301 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3361 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3421 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3481 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3541 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3601 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
     3661 ccggactcag atctcgaggc caccatgccc aagaagaagc gcaaggtggg acgcgtctgc
     3721 aggatatcaa gcttgcggta ccgcgggccc gggatcgcca ccatggacaa gaagtacagc
     3781 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     3841 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     3901 ctgatcggcg ccctgctgtt cgacagcgga gaaacagccg aggccacccg gctgaagaga
     3961 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     4021 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     4081 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     4141 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     4201 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     4261 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     4321 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     4381 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     4441 gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc cctgagcctg
     4501 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     4561 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     4621 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     4681 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     4741 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     4801 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat cgatggcgga
     4861 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     4921 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     4981 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     5041 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     5101 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     5161 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgcc
     5221 agcgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     5281 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtacaacga gctgaccaaa
     5341 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaaa
     5401 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     5461 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     5521 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     5581 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     5641 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     5701 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     5761 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     5821 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     5881 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     5941 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     6001 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     6061 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     6121 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     6181 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     6241 gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc tcagagcttt
     6301 ctgaaggacg actccatcga taacaaagtg ctgactcgga gcgacaagaa ccggggcaag
     6361 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcgccagctg
     6421 ctgaatgcca agctgattac ccagaggaag ttcgacaatc tgaccaaggc cgagagaggc
     6481 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     6541 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaac
     6601 gacaaactga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     6661 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     6721 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     6781 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     6841 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     6901 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     6961 ggcgaaacag gcgagatcgt gtgggataag ggccgggact ttgccaccgt gcggaaagtg
     7021 ctgtctatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     7081 aaagagtcta tcctgcccaa gaggaacagc gacaagctga tcgccagaaa gaaggactgg
     7141 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     7201 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     7261 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     7321 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     7381 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     7441 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     7501 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa acactacctg
     7561 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     7621 ctggacaagg tgctgagcgc ctacaacaag cacagagaca agcctatcag agagcaggcc
     7681 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     7741 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     7801 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     7861 ggcgacgcct atccctatga cgtgcccgat tatgcaccgg tcccaaaaaa gaaaagaaaa
     7921 gtaccggtcg ccacccgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
     7981 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     8041 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     8101 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     8161 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     8221 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     8281 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     8341 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     8401 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     8461 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     8521 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     8581 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     8641 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     8701 aagactagtg aggccagcgg gcgcgccatt ttcaaaccag aagaactacg acaggcactg
     8761 atgccaactt tggaggcact ttaccgtcag gatccagaat cccttccctt tcgtcaacct
     8821 gtggaccctc agcttttagg aatccctgat tactttgata ttgtgaagag ccccatggat
     8881 ctttctacca ttaagaggaa gttagacact ggacagtatc aggagccctg gcagtatgtc
     8941 gatgatattt ggcttatgtt caataatgcc tggttatata accggaaaac atcacgggta
     9001 tacaaatact gctccaagct ctctgaggtc tttgaacaag aaattgaccc agtgatgcaa
     9061 agccttggat actgttgtgg cagaaagttg gagttctctc cacagacact gtgttgctac
     9121 ggcaaacagt tgtgcacaat acctcgtgat gccacttatt acagttacca gaacaggtat
     9181 catttctgtg agaagtgttt caatgagatc caaggggaga gcgtttcttt gggggatggc
     9241 ccttcccagc ctcaaactac aataaataaa gaacaatttt ccaagagaaa aaatgacaca
     9301 ctggatcctg aactgtttgt tgaatgtaca gagtgcggaa gaaagatgca tcagatctgt
     9361 gtccttcacc atgagatcat ctggcctgct ggattcgtct gtgatggctg tttaaagaaa
     9421 agtgcacgaa ctaggaaaga aaataagttt tctgctaaaa ggttgccatc taccagactt
     9481 ggcacctttc tagagaatcg tgtgaatgac tttctgaggc gacagaatca ccctgagtca
     9541 ggagaggtca ctgttagagt agttcatgct tctgacaaaa ccgtggaagt aaaaccaggc
     9601 atgaaagcaa ggtttgtgga cagtggagag atggcagaat cctttccata ccgaaccaaa
     9661 gccctctttg cctttgaaga aattgatggt gttgacctgt gcttctttgg catgcatgtt
     9721 caagagtatg gctctgactg ccctccaccc aaccagagga gagtatacat atcttacctc
     9781 gatagtgttc atttcttccg tcctaaatgc ttgaggactg cagtctatca tgaaatccta
     9841 attggatatt tagaatatgt caagaaatta ggttacacaa cagggcatat ttgggcatgt
     9901 ccaccaagtg agggagatga ttatatcttc cattgccatc ctcctgacca gaagataccc
     9961 aagcccaagc gactgcagga atggtacaaa aaaatgcttg acaaggctgt atcagagcgt
    10021 attgtccatg actacaagga tatttttaaa caagctactg aagatagatt aacaagtgca
    10081 aaggaattgc cttatttcga gggtgatttc tggcccaatg ttctggaaga aagcattaag
    10141 gaactggaac aggaggaaga agagagaaaa cgagaggaaa acaccagcaa tgaaagcaca
    10201 gatgtgacca agggagacag caaaaatgct aaaaagaaga ataataagaa aaccagcaaa
    10261 aataagagca gcctgagtag gggcaacaag aagaaacccg ggatgcccaa tgtatctaac
    10321 gacctctcac agaaactata tgccaccatg gagaagcata aagaggtctt ctttgtgatc
    10381 cgcctcattg ctggccctgc tgccaactcc ctgcctccca ttgttgatcc tgatcctctc
    10441 atcccctgcg atctgatgga tggtcgggat gcgtttctca cgctggcaag ggacaagcac
    10501 ctggagttct cttcactccg aagagcccag tggtccacca tgtgcatgct ggtggagctg
    10561 cacacgcaga gccaggacta cccgtacgac gttccggact acgcttcttg aaacgttcag
    10621 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
    10681 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    10741 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10861 cttaaggcgt aaa
//
