LOCUS       sequence-36416-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A
  TITLE     Biochemical analysis of the canonical model for the mammalian 
            circadian clock.
  JOURNAL   J Biol Chem. 2011 Jul 22;286(29):25891-902. doi: 
            10.1074/jbc.M111.254680. Epub 2011 May 25.
  PUBMED    21613214
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.254680"; journalName: "J Biol Chem"; date: 
            "2011-07-22- 22"; volume: "286"; issue: "29"; pages: "25891-902"
COMMENT     Sequence Label: pBC2FG
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      167..176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttatgtttcg tacagttttg tataaaaaaa cctataaata ttccggatta ttcataccgt
       61 cccaccatcg ggcgcggatc tcggtccgaa accatgtcgt actaccatca ccatcaccat
      121 cacgattacg atatcccaac gaccgaaaac ctgtattttc agggcgccat ggcggcggct
      181 gctgtggtgg cagcgacggt ccccgcgcaa tcgatgggcg cggacggcgc gtcctcggtg
      241 cactggttcc gcaaaggact acggctccac gacaaccccg cgctgctagc tgccgtgcgc
      301 ggggcgcgct gtgtgcgctg cgtctacatc ctcgacccgt ggttcgcggc ctcctcgtct
      361 gtgggcatca accgatggag gttcctactg caatctctgg aagatctgga cacaagctta
      421 agaaagctga attcgcgtct gtttgtagtc cggggacagc cagctgatgt gttcccaagg
      481 ctgttcaagg aatggggggt gacccgtttg acctttgaat atgactctga accctttggg
      541 aaagaacggg atgcagccat tatgaagatg gccaaggagg ctggcgtgga ggtggtgact
      601 gagaactctc acaccctcta tgacctagac agaatcatcg aactgaatgg gcagaaacca
      661 ccccttacct acaagcgctt tcaggccctc atcagccgca tggagctgcc caagaagccc
      721 gcggtggctg tgagcagcca gcagatggag agctgcagag ctgagatcca ggagaaccat
      781 gacgacacct atggcgtgcc ttccctggag gagctgggat tccccacgga aggacttggc
      841 ccagctgttt ggcaagga
//
