LOCUS       sequence-36417-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A
  TITLE     Biochemical analysis of the canonical model for the mammalian 
            circadian clock.
  JOURNAL   J Biol Chem. 2011 Jul 22;286(29):25891-902. doi: 
            10.1074/jbc.M111.254680. Epub 2011 May 25.
  PUBMED    21613214
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.254680"; journalName: "J Biol Chem"; date: 
            "2011-07-22- 22"; volume: "286"; issue: "29"; pages: "25891-902"
COMMENT     Sequence Label: pMC1SG5
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(75..104)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(111..134)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tataagctgc ataacagttc tgctttataa gatctggatc aatgatgatg atgatgatgg
       61 tcgacggcgc tattcagatc ctcttctgag atgagttttt gttctagaaa cttgtcgtca
      121 tcgtctttgt agtccatggc ggccgcgtta ctgctctgcc gctggacttt ggggccgaca
      181 ctctgggcat cctcttcctg actaggacgc ttcccactgc tgaggccggt gcccgcggag
      241 cttctccctt gcttgagtga gtgagtctgc tgactgtccc cgtgagcata gtgtaaaata
      301 ccacttcctt gagagcaatt tccaccgcta cagctcggga cgttctctcc aggagcatag
      361 cccatgagcc ctccattccc gctgctgcta caactcggga cattctctcc aggagcatag
      421 cccatgagcc ctccattccc attagagtta gaagggaccg aggcgagaag acctagccct
      481 ctgtaccggg aaagctgctg atagatctgc ttcattcttt caatattcag tctgcttgcc
      541 tcagcatggt tcaccatcgg tttggggtaa ttaactccta tcaaacactt ggcaaccttc
      601 tggatgcctt ctggtgcatt ccaaggatcg tagatatatt ttgcagggaa gcctcttagg
      661 acaggtaaat aacgcctaat atagtctcca ttgggatctg tcctcctacc aaaacccaca
      721 gggcagtagc agtgaaaaaa ttgctgaaaa aaggaactgc aggacagcca catccaactt
      781 ccagcattta tgctccaatc tgcatcaagc agtaactctt caaagacctt catcccttct
      841 tcccagctga tccacaggtc accacgagtc aggaaacagg caaccgcgtg tctggctaaa
      901 tggtggatcc agccctcctg acgaagctga gtcatgatgg cgtcaatcc
//
