LOCUS       sequence-36418-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A
  TITLE     Biochemical analysis of the canonical model for the mammalian 
            circadian clock.
  JOURNAL   J Biol Chem. 2011 Jul 22;286(29):25891-902. doi: 
            10.1074/jbc.M111.254680. Epub 2011 May 25.
  PUBMED    21613214
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.254680"; journalName: "J Biol Chem"; date: 
            "2011-07-22- 22"; volume: "286"; issue: "29"; pages: "25891-902"
COMMENT     Sequence Label: pMC2SG5
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..58)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(74..103)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(110..133)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taagctgcat aacagttctg ctttaataag atctggatca atgatgatga tgatgatggt
       61 cgacggcgct attcagatcc tcttctgaga tgagtttttg ttctagaaac ttgtcgtcat
      121 cgtctttgta gtccatggcg gccgcggagt ccttgcttgc tggctcttgg gtaggcatct
      181 ccgtcactct agcccgcttg gtcagttctt caccaggagg ttcctcggct gcttccagct
      241 tgcgtttggg ggaagctggg ccactggata gtgctctggg gcctgtgttg ctgatgctcc
      301 cagcttggct tgaaccaggc tctgccacag ggtgactgag gtcttccaca caggaaggga
      361 cagatgccaa tagacagagt ccccggtatc tcgacagctg ttggtagatc tgcttcattc
      421 gttcaatgtt gagccgacta gtctctgcat gattgacgat gggccgtggg tagtccacgc
      481 caatgatgca cttggcagcc ttctgaactg actcgggggc attccagggc tcatagatgt
      541 atcgagaggg gaagcctttc agtttgggca ggtaccgccg gatgtagtcc ccactggggt
      601 ctgtacgtcg gccgaagccc acagggcagt agcagtggaa gaattgttgg aagaaagcac
      661 tgcaggacag ccacatccag ctgcctgcat tcacactgaa atcggcatcc aggagcagct
      721 cgtcaaatac ccggaccccg ctctcccagc tgacccagag gtccccgcgg gtgaggaagc
      781 aggccacagc gtgccgggcc aggtggtgga tccagccctc ctgcctcagt tgggtcatga
      841 tggcgtcaat ccaagggaag cctgtcttgc cctcggccca cttggccagg gcttcggggt
      901 tgcggtc
//
