LOCUS       sequence-364193-        9930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSwap plasmid part with H2_beta homology arms and gentamycin 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Teufel M, Klein CA, Mager M, Sobetzko P
  TITLE     A multifunctional system for genome editing and large-scale 
            interspecies gene transfer.
  JOURNAL   Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1.
  PUBMED    35701417
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-14"; volume: "13(1):3430. doi"; pages: " 
            10.1038/s41467-022-30843-1"
COMMENT     Sequence Label: pSWAP_Entry_H2_beta_GM
FEATURES             Location/Qualifiers
     source          1..9930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             60..593
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     terminator      complement(640..711)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_RNA        complement(731..806)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    837..855
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    862..880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(1100..1405)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1202..1221)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1747..2070)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     1752..1771
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1965..1987
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1979..1996
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1980..1996
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1997..2053
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2066..2082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2066..2082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2079..2101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2090..2106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2114..2120,2121..2138,2139..2144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2159..2180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2327..2361
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     terminator      complement(2895..2938)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(3083..3671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3163..3182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4130..4603
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(4676..5647)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(5647..6822)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    7148..7398
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(7401..8156)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      8247..8466
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     terminator      complement(9306..9376)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_RNA        complement(9396..9471)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    9502..9520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9527..9545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     oriT            9655..9763
                     /note="incP origin of transfer"
     promoter        join(9924..9930,1..41)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
       61 tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg
      121 gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc
      181 gggctgctct tgatcttttc ggtcgtgagt tcggagatgt agccacctac tcccaacatc
      241 agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatt gcgcttgctg
      301 ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc
      361 agccgcgtag tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg
      421 gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg
      481 tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg
      541 gcatacggga agaagtgatg cactttgata tcgacccaag caccgccacc taactatatt
      601 accctgttat ccctagcgta actagcgtga gactcacttg agagcgttca ccgacaaaca
      661 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc
      721 ttgaaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt
      781 taacttgcta tttctagctc taaaacctag tagctacggg catatcgtgc tcagtatctc
      841 tatcactgat agggatgtca atctctatca ctgataggga agaattccag aaatcatcct
      901 tagcgaaagc ttgcctggac tcacaaagaa aaaacgcccg gtgtgcaaga ccgagcgttc
      961 tgaacaacag cctgttgaca attgctacga tcgatagcga atatagagcg cgagaccctt
     1021 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     1081 ggtgtcgggg ctggcttaat tatattcccc agaacatcag gttaatggcg tttttgatgt
     1141 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagaca ggcacactgg
     1201 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     1261 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     1321 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     1381 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     1441 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     1501 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     1561 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     1621 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     1681 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     1741 acgcgactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     1801 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     1861 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1921 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1981 taaaacgacg gccagtgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
     2041 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2101 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2161 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2221 tgtcgtgcca gctgcccata ccactgggtc tcactccccg tcaccgtttg gaccttggtt
     2281 cgatcagcta ccttgcagcg acaaatattg atagcctgaa tcagtattga cagctagctc
     2341 agtcctaggg actatgctag caaacactac tgctctttaa caatttatca gatccataag
     2401 gaggaacata atgcactcga gcacaaaata taataaagag ttcttactct acttagcagg
     2461 gtttgtagac ggtgacggta gcataatcgc tcaaattaag cctaatcagt cttataaatt
     2521 taagcatcag ctatcactca cgttccaagt cacgcaaaag acacagaggc gttggttttt
     2581 agacaaatta gtggatgaaa ttggggttgg ttatgtaaga gataggggta gcgtttcgga
     2641 ttatattcta agcgaaatca agcctttgca taatttttta acacaactac aaccttttct
     2701 aaaactaaaa caaaaacaag caaatttagt tttaaaaatt attgaacaac ttccgtcagc
     2761 aaaagaatcc ccggacaaat tcttagaagt ttgtacatgg gtggatcaaa ttgcagctct
     2821 gaatgattcg aacacgcgta aaacaacttc tgaaaccgtt cgtgctgtgc tagacagttt
     2881 atccggataa gcttataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatcg
     2941 ctcttggtac tagcgcggca aaaccccgcc gaagcggggt tttcggcgac caatgtcttc
     3001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctggaa
     3841 gacgagggaa ccatgactac tgtccggtcc aacagatccg agcaggtcga cagcgacaca
     3901 cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tgggtaacca ggtattttgt
     3961 ccacataacc gtgcgcaaaa tgttgtggat aagcaggaca cagcagcaat ccacagcagg
     4021 catacaaccg cacaccgagg ttactccgtt ctacaggtta cgacgacatg tcaatacttg
     4081 cccttgacag gcattgatgg aatcgtagtc tcacgctgat agtctgatcg acaatacaag
     4141 tgggaccgtg gtcccagacc gataatcaga ccgacaacac gagtgggatc gtggtcccag
     4201 actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga
     4261 cgatacgagt gggaccgtgg ttccagacta ataatcagac cgacgatacg agtgggaccg
     4321 tggtcccaga ctaataatca gaccgacgat acgagtggga ccatggtccc agactaataa
     4381 tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc gacgatacga
     4441 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca
     4501 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
     4561 acgatacaag tggaacagtg ggcccagaga gaatattcag gccagttatg ctttctggcc
     4621 tgtaacaaag gacattaagt aaagacagat aaacgtagac taaaacgtgg tcgcatcagg
     4681 gtgctggctt ttcaagttcc ttaagaatgg cctcaatttt ctctatacac tcagttggaa
     4741 cacgtgacct gtccaggtta agcaccattt tatcgccctt atacaatact gtcgctccag
     4801 gagcaaactg atgtcgtgag cttaaactag ttcttgatgc agatgacgtt ttaagcacag
     4861 aagttaaaag agtgataact tcttcagctt caaatatcac cccagctttt ttctgctcat
     4921 gaaggttaga tgcctgctgc ttaagtaatt cctctttatc tgtaaaggct ttttgaagtg
     4981 catcacctga ccgggcagat agttcaccgg ggtgagaaaa aagagcaaca actgatttag
     5041 gcaatttggc ggtgttgata cagcgggtaa taatcttacg tgaaatattt tccgcatcag
     5101 ccagcgcaga aatatttcca gcaaattcat tctgcaatcg gcttgcataa cgctgaccac
     5161 gttcataagc acttgttggg cgataatcgt tacccaatct ggataatgca gccatctgct
     5221 catcatccag ctcgccaacc agaacacgat aatcactttc ggtaagtgca gcagctttac
     5281 gacggcgact cccatcggca atttctatga caccagatac tcttcgaccg aacgccggtg
     5341 tctgttgacc agtcagtaga aaagaaggga tgagatcatc cagtgcgtcc tcagtaagca
     5401 gctcctggtc acgttcatta cctgaccata cccgagaggt tttctcaaca ctatcacccc
     5461 ggagcacttc aagagtaaac ttcacatccc gaccacatac aggcaaagta atggcattac
     5521 cgcgagccat tactcctacg cgcgcaatta acgaatccac catcggggca gctggtgtcg
     5581 ataacgaagt atcttcaacc ggttgagtat tgagcgtatg ttttggaata acaggcgcac
     5641 gcttcattat ctaatctccc agcgtggttt aatcagacga tcgaaaattt cattgcagac
     5701 aggttcccaa atagaaagag catttctcca ggcaccagtt gaactgcgtt gatcaatggc
     5761 ctgttcaaaa acagttctca tccggatctg acctttacca acttcatccg tttcacgtac
     5821 aacatttttt agaaccatgc ttccccaggc atcccgaatt tgctcctcca tccacgggga
     5881 ctgagagcca ttactattgc tgtatttggt aagcaaaata cgtacatcag gctcgaaccc
     5941 tttaagatca acgttcttga gcagatcacg aagcatatcg aaaaactgca gtgcggaggt
     6001 gtagtcaaac aactcagcag gcgtgggaac aatcagcaca tcagcagcac atacgacatt
     6061 aatcgtgccg atacccaggt taggcgcgct gtcaataact atgacatcat agtcatgagc
     6121 aacagtttca atggccagtc ggagcatcag gtgtggatcg gtgggcagtt taccttcatc
     6181 aaatttgccc attaactcag tttcaatacg gtgcagagcc agacaggaag gaataatgtc
     6241 aagccccggc cagcaagtgg gctttattgc ataagtgaca tcgtcctttt ccccaagata
     6301 gaaaggcagg agagtatctt ctgcatgaat atgaagatct ggtacccatc cgtgatacat
     6361 tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa acacgtagcc ccttcagagc
     6421 cagatcctga gcaagatgaa cagaaactga ggttttgtaa acgccacctt tatgggcagc
     6481 aaccccgatc accggtggaa atacgtcctc agcacgtcgc aatcgcgtac caaacacatc
     6541 acgcatatga ttaatttgtt caattgtata accaacacgt tgctcaaccc gtcctcgaat
     6601 ttccatatcc gggtgcggta gtcgccctgc tttctcggca tctctgatag cctgagaaga
     6661 aaccccaact aaatccgctg cttcacctat tctccagcgc cgggttattt tcctcgcttc
     6721 cgggctgtca tcattaaact gtgcaatggc gatagccttc gtcatttcat gaccagcgtt
     6781 tatgcactgg ttaagtgttt ccatgagttt cattctgaac atcctttaat cattgctttg
     6841 cgttttttta ttaaatcttg caatttactg caaagcaaca acaaaatcgc aaagtcatca
     6901 aaaaaccgca aagttgttta aaataagagc aacactacaa aaggagataa gaacaccaca
     6961 tacctcagtc acttattatc actagcgctc gccgcagccg tgtaaccgag catagcgagc
     7021 gaactggcga ggaagcaaag aagaactgtt ctgtcagata gctcttacgc tcagcgcaag
     7081 aagaaatatc caccgtggga aaaactccag gtagaggtac acacgcggat agccaattca
     7141 gagtaataaa ctgtgataat caaccctcat caatgatgac gaactaaccc ccgatatcag
     7201 gtcacatgac gaagggaaag agaaggaaat caactgtgac aaactgccct caaatttggc
     7261 ttccttaaaa attacagttc aaaaagtatg agaaaatcca tgcaggctga aggaaacagc
     7321 aaaactgtga caaattaccc tcagtaggtc agaacaaatg tgacgaacca ccctcaaatc
     7381 tgtgacagat aaccctcaga ctatcctgtc gtcatggaag tgatatcgcg gaaggaaaat
     7441 acgatatgag tcgtctggcg gcctttcttt ttctcaatgt atgagaggcg cattggagtt
     7501 ctgctgttga tctcattaac acagacctgc aggaagcggc ggcggaagtc aggcatacgc
     7561 tggtaacttt gaggcagctg gtaacgctct atgatccagt cgattttcag agacacgatg
     7621 cctgagccat ccggcttacg atactgacac agggattcgt ataaacgcat ggcatacgga
     7681 ttggtgattt cttttgtttc actaagccga aactgcgtaa accggttctg taacccgata
     7741 aagaagggaa tgagatatgg gttgatatgt acactgtaaa gccctctgga tggactgtgc
     7801 gcacgtttga taaaccaagg aaaagattca tagccttttt catcgccggc atcctcttca
     7861 gggcgataaa aaaccacttc cttccccgcg aaactcttca atgcctgccg tatatcctta
     7921 ctggcttccg cagaggtcaa tccgaatatt tcagcatatt tagcaacatg gatctcgcag
     7981 ataccgtcat gttcctgtag ggtgccatca gattttctga tctggtcaac gaacagatac
     8041 agcatacgtt tttgatcccg ggagagacta tatgccgcct cagtgaggtc gtttgactgg
     8101 acgattcgcg ggctattttt acgtttcttg tgattgataa ccgctgtttc cgccatgaca
     8161 gatccatgtg aagtgtgaca agtttttaga ttgtcacact aaataaaaaa gagtcaataa
     8221 gcagggataa ctttgtgaaa aaacagcttc ttctgagggc aatttgtcac agggttaagg
     8281 gcaatttgtc acagacagga ctgtcatttg agggtgattt gtcacactga aagggcaatt
     8341 tgtcacaaca ccttctctag aaccagcatg gataaaggcc tacaaggcgc tctaaaaaag
     8401 aagatctaaa aactataaaa aaaataatta taaaaatatc cccgtggata agtggataac
     8461 cccaagggaa gttttttcag gcatcgtgtg taagcagaat atataagtgc tgttccctgg
     8521 tgcttcctcg ctcactcgag ggcttcgccc tgtcgctcga ctgcggcgag cactactggc
     8581 tgtaaaagga cagaccacat catggttctg tgttcattag gttgttctgt ccattgctga
     8641 cataatccgc tccacttcaa cgtaacaccg cacgaagatt tctattgttc ctgaaggcat
     8701 attcaaatcg ttttcgttac cgcttgcagg catcatgaca gaacactact tcctataaac
     8761 gctacacagg ctcctgagat taataatgcg gatctctacg ataatgggag attttcccga
     8821 ctgtttcgtt cgcttctcag tggataacag ccagcttctc tgtttaacag acaaaaacag
     8881 catatccact cagttccaca tttccatata aaggccaagg catttattct caggataatt
     8941 gtttcagcat cgcaaccgca tcagactccg gcatcgcaaa ctgcacccgg agccgggcag
     9001 ccacatccag cgcaaaaacc ttcgtgtaga cttccgttga actgatggac ttatgtccca
     9061 tcaggctttg cagaactttc agcggtatac cggcatacag catgtgcatc gcataggaat
     9121 ggcggaacgt atgtggtgtg accggaacag agaacgtcac accgtcagca gcagcggcgg
     9181 caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag atccgcgctt
     9241 tctctgtcct tcctgtgcga cggttacgcc gctccatcga gtgagcgagg aagcggaata
     9301 tatccagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     9361 cctttcgttt tatttgatgc ctggcttgaa aaaaagcacc gactcggtgc cactttttca
     9421 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa ctcaccgttt
     9481 ggaccttggt tgtgctcagt atctctatca ctgataggga tgtcaatctc tatcactgat
     9541 agggaagaat tccagaaatc atccttagcg aaagcttgcc tggactcaca aagaaaaaac
     9601 gcccggtgtg caagaccgag cgttctgaac aacatcgagc acccgggagc ttatcggcca
     9661 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     9721 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     9781 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     9841 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaagtat
     9901 accttaaggg ctagcggtgg cctgttgaca
//
