LOCUS       sequence-364197-        7763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic PHR domain coupled to GFP and VPR activation domain; 
            binds to CIBN upon blue light exposure.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
  TITLE     Transcription activation is enhanced by multivalent interactions 
            independent of phase separation.
  JOURNAL   Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017.
  PUBMED    35537448
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017."
COMMENT     Sequence Label: PHR-GFP-VPR
FEATURES             Location/Qualifiers
     source          1..7763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        60..363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        364..567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     518..538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             624..2117
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(2127..2129,2130..2132,2133..2843)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2127..2129,2130..2132,2133..2843)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2172..2193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2433..2452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2780..2801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2877..2897
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2964..3113
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3138..3158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3588..3944
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             3963..4532
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     polyA_signal    4550..4671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4587..4606)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4641..4660
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4678..5133)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4815..4836)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5027..5046
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5160..5264
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5266..5623
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5266..5457
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5289..5309)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5474..5609
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5658..6452
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5658..6452
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5712..5731)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6322..6341
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6640..6659)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6684..6731
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7060..7648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7549..7568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
      601 cggactcaga tctcgaggcc accatgaaga tggacaaaaa gactatagtt tggtttagaa
      661 gagacctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa ggatctgttt
      721 ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga agagcttcaa
      781 gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct cttggatctg
      841 acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt atccgcgtta
      901 ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta gttcgggacc
      961 ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac aatggagatc
     1021 tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg agtttcaatt
     1081 cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct cctccttggc
     1141 ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt gaagaactag
     1201 ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct tggtctccag
     1261 gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg atagattatg
     1321 caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg tatctccatt
     1381 tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa attatatggg
     1441 caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt
     1501 taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag caatcgttgt
     1561 tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag gcctggagac
     1621 aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg gctaccggat
     1681 ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt cttctccttc
     1741 catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat ttggaatgtg
     1801 acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag cttgatcgct
     1861 tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac ataaggcaat
     1921 ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg gacgctcctt
     1981 taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag
     2041 acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt gaagcacaga
     2101 tcatgatcgg agcagcagcc cgggtcatgg tgagcaaggg cgaggagctg ttcaccgggg
     2161 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     2221 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     2281 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     2341 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2401 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2461 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2521 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     2581 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     2641 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2701 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     2761 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2821 tcggcatgga cgagctgtac aagagcggcc gcagggggcg tcgactgggt acccctccta
     2881 agaagaagag gaaggttgcc cccaccatgg ataaaaagaa gagaaaggcc cccccgaccg
     2941 atgtcagcct gggggacgag ctcgacgcat tggacgattt tgatctggat atgctgggaa
     3001 gtgacgccct cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg
     3061 acctcgacat gctcggcagt gacgcccttg atgatttcga cctggacatg ctgattaact
     3121 ctagaagttc cggatctccg aaaaagaaac gcaaagttgg tagccagtac ctgcccgaca
     3181 ccgacgaccg gcaccggatc gaggaaaagc ggaagcggac ctacgagaca ttcaagagca
     3241 tcatgaagaa gtcccccttc agcggcccca ccgaccctag acctccacct agaagaatcg
     3301 ccgtgcccag cagatccagc gccagcgtgc caaaacctgc cccccagcct taccccttca
     3361 ccagcagcct gagcaccatc aactacgacg agttccctac catggtgttc cccagcggcc
     3421 agatctctca ggcctctgct ctggctccag cccctcctca ggtgctgcct caggctcctg
     3481 ctcctgcacc agctccagcc atggtgtctg cactggctca ggcaccagca cccgtgcctg
     3541 tgctggctcc tggacctcca caggctgtgg ctccaccagc ccctaaacct acacaggccg
     3601 gcgagggcac actgtctgaa gctctgctgc agctgcagtt cgacgacgag gatctgggag
     3661 ccctgctggg aaacagcacc gatcctgccg tgttcaccga cctggccagc gtggacaaca
     3721 gcgagttcca gcagctgctg aaccagggca tccctgtggc ccctcacacc accgagccca
     3781 tgctgatgga ataccccgag gccatcaccc ggctcgtgac aggcgctcag aggcctcctg
     3841 atccagctcc tgcccctctg ggagcaccag gcctgcctaa tggactgctg tctggcgacg
     3901 aggacttcag ctctatcgcc gatatggatt tctcagcctt gctgggctct ggcagcggca
     3961 gccgggattc cagggaaggg atgtttttgc cgaagcctga ggccggctcc gctattagtg
     4021 acgtgtttga gggccgcgag gtgtgccagc caaaacgaat ccggccattt catcctccag
     4081 gaagtccatg ggccaaccgc ccactccccg ccagcctcgc accaacacca accggtccag
     4141 tacatgagcc agtcgggtca ctgaccccgg caccagtccc tcagccactg gatccagcgc
     4201 ccgcagtgac tcccgaggcc agtcacctgt tggaggatcc cgatgaagag acgagccagg
     4261 ctgtcaaagc ccttcgggag atggccgata ctgtgattcc ccagaaggaa gaggctgcaa
     4321 tctgtggcca aatggacctt tcccatccgc ccccaagggg ccatctggat gagctgacaa
     4381 ccacacttga gtccatgacc gaggatctga acctggactc acccctgacc ccggaattga
     4441 acgagattct ggataccttc ctgaacgacg agtgcctctt gcatgccatg catatcagca
     4501 caggactgtc catcttcgac acatctctgt tttaacaatt gttgttgtta acttgtttat
     4561 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4621 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     4681 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4741 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4801 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4861 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4921 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4981 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5041 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5101 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     5161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     5281 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5341 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5401 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5461 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5521 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5581 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     5641 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5701 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5761 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5821 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5881 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5941 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6001 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6061 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6121 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6181 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6241 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6301 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6361 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6421 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6481 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6541 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6601 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     6661 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     6721 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     6781 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     6841 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     6901 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6961 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7201 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7441 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7681 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7741 taaccgtatt accgccatgc att
//
