LOCUS       sequence-36420-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A
  TITLE     Biochemical analysis of the canonical model for the mammalian 
            circadian clock.
  JOURNAL   J Biol Chem. 2011 Jul 22;286(29):25891-902. doi: 
            10.1074/jbc.M111.254680. Epub 2011 May 25.
  PUBMED    21613214
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.254680"; journalName: "J Biol Chem"; date: 
            "2011-07-22- 22"; volume: "286"; issue: "29"; pages: "25891-902"
COMMENT     Sequence Label: pBC1FG
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             142..162
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgtttcgtac agttttgtat aaaaaaacct ataaatattc cggattattc ataccgtccc
       61 accatcgggc gcggatctcg gtccgaaacc atgtcgtact accatcacca tcaccatcac
      121 gattacgata tcccaacgac cgaaaacctg tattttcagg gcgccatggg ggtgaacgcc
      181 gtgcactggt tccgaaaggg actccggctc cacgacaacc ccgccctgaa ggagtgcatc
      241 cagggcgccg acaccatccg ctgcgtctat atcctcgacc cctggttcgc cggctcttcc
      301 aacgtgggca tcaacaggtg gcgatttttg cttcagtgtc ttgaggatct tgatgccaat
      361 ctacgaaaat taaattctcg tctgtttgtg attcggggac agccagctga tgtatttccc
      421 aggcttttca aggaatggaa catcactaaa ctctcaattg agtatgattc tgagcctttt
      481 gggaaggaac gagatgcagc tatcaagaag ctggctactg aggctggcgt ggaagtcatc
      541 gtgcgcattt cacatacact gtatgacctg gacaagatca tagaactcaa tggcggacag
      601 ccacctctaa catataaaag gtttcagact ctcgtcagca agatggagcc actggagatg
      661 ccagcagaca ccatcacatc agatgtgata ggaaagtgca tgacccctct gtctgatgac
      721 catgatgaga aatatggcgt tccttccctg gaagagctcg gctttgatac agatggcctg
      781 tcctctgcag tgtggccagg aggagaaact gaggcactta cacg
//
