LOCUS       sequence-36421-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F3-GAT
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..45
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ttgcaaggat ctgccaccat ggctccaaag aagaagcgta aggtagacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gggtacccgc
      121 ccatatgctt gccctgtcga gtcctgtgac cgccgcttca gccgtagcga cgcgctcacc
      181 cgccacatcc ggattcacac aggccagaag cccttccagt gtcgcatctg catgaggaac
      241 ttcagccgtt ctgaccacct cacccgccac atccgcaccc acacaggcga gaagcctttt
      301 gcatgcgaca tttgcggcag gaagttcgcg cttagtttca acctcacccg ccacaccaag
      361 atccacacag gtggatccca actagtcaaa agtgaactgg aggagaagaa atctgaactt
      421 cgtcataaat tgaaatatgt gcctcatgaa tatattgaat taattgaaat tgccagaaat
      481 tccactcagg atagaattct tgaaatgaag gtaatggaat tttttatgaa agtttatgga
      541 tatagaggta aacatttggg tggatcaagg aaaccggacg gagcaattta tactgtcgga
      601 tctcctattg attacggtgt gatcgtggat actaaagctt atagcggagg ttataatctg
      661 ccaattggcc aagcag
//
