LOCUS       sequence-364236-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fourth-generation FRET-based Indicator of cAMP Using Epac. 
            Genetically encoded cAMP indicator. Contains high-sensitivity Epac 
            Q270E mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke 
            M, Rangamani P, Taylor SS, Mehta S, Zhang J
  TITLE     Phase Separation of a PKA Regulatory Subunit Controls cAMP 
            Compartmentation and Oncogenic Signaling.
  JOURNAL   Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 
            10.1016/j.cell.2020.07.043. Epub 2020 Aug 25.
  PUBMED    32846158
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07.043"; journalName: "Cell"; date: 
            "2020-09-17- 17"; volume: "182"; issue: "6"; pages: "1531-1544.e15"
COMMENT     Sequence Label: pcDNA3-ICUE4
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             943..975
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(979..987,988..1002)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(1009..1011,1012..1014,1015..1725)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1009..1011,1012..1014,1015..1725)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(1009..1011,1012..1014,1015..1725)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(4090..4092,4093..4095,4096..4608)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     promoter        complement(4738..4756)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4739..4756)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4776..4793)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4782..5006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5052..5480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5139..5158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5349..5370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5489..5509)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5494..5823
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5674..5809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5736..5755
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5890..6684
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5944..5963)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6554..6573
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6858..6979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6895..6914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6949..6968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7028..7044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7028..7044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7041..7063)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7052..7068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7076..7082,7083..7100,7101..7106))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7121..7142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7259..7276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7430..8018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7510..7529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8189..8980,8981..9049))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8812..8831
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9050..9154)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat ggtgagcaag
     1021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1081 ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1201 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1261 ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac
     1321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1441 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1501 cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1621 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1681 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaaggctgg tacccccgtg
     1741 ggaactcatg agatggagga ggagttggcc gaagctgtgg ccctgctctc ccagcggggg
     1801 cctgacgccc tgctcactgt ggcacttcga aagcccccag gtcagcgcac ggatgaagag
     1861 ctggacctca tctttgagga gctgctgcac atcaaggctg tggcccacct ctccaactcg
     1921 gtgaagcgag aattagcggc tgttctgctc tttgaaccac acagcaaggc agggaccgtg
     1981 ttgttcagcc agggggacaa gggcacttcg tggtacatta tctggaaggg atctgtcaac
     2041 gtggtgaccc atggcaaggg gctggtgacc accctgcatg agggagatga ttttggagaa
     2101 ctggctctgg tgaatgatgc accccgggca gccaccatca tcctgcgaga agacaactgt
     2161 catttcctgc gtgtggacaa gcaggacttc aaccgtatca tcaaggatgt ggaggcaaag
     2221 accatgcggc tggaagaaca tggcaaagtg gtgctggtgc tggagagagc ctctcagggc
     2281 gccggccctt cccgaccccc aaccccaggc aggaaccggt atacagtgat gtctggcacc
     2341 ccagagaaga tcctagagct tctgttggag gccatgggac cagattccag tgctcatgac
     2401 caaacagaga cattcctcag cgacttcctc ctgacccaca gggtcttcat gcccagcgcc
     2461 caactctgcg ctgcccttct gcaccacttc catgtggagc ctgcgggtgg cagcgagcag
     2521 gagcgcagca cctacgtctg caacaagagg cagcagatct tgcggctggt cagccagtgg
     2581 gtggccctgt atggctccat gctccacact gaccctgtgg ccaccagctt cctccagaaa
     2641 ctctcagacc tggtgggcag ggacacccga ctcagcaacc tgctgaggga gcagtggcca
     2701 gagaggcggc gatgccacag gttggagaat ggctgtggga atgcatctcc tcagatgaag
     2761 gcccggaact tgcctgtttg gctccccaac caggacgagc cccttcctgg cagcagctgt
     2821 gccatccaag ttggggataa agtcccctat gacatctgcc ggccagacca ctcagtgttg
     2881 accctgcagc tgcctgtgac agcctccgtg agagaggtga tggcagcgtt ggcccaggag
     2941 gatggctgga ccaaggggca ggtgctggtg aaggtcaatt ctgcaggtga tgccattggc
     3001 ctgcagccag atgcccgtgg tgtggccaca tctctggggc tcaatgagcg tctctttgtt
     3061 gtcaacccac aggaagcgca tgagctgatc ccacaccctg accagctggg gcccactgtg
     3121 ggctctgctg aggggctgga cctggtgagt gccaaggacc tggcaggcca gctgacggac
     3181 cacgactgga gcctcttcaa cagtatccac caggtggagc tgatccacta tgtgctgggc
     3241 ccccagcatc tgcgggatgt caccaccgcc aacctggagc gcttcatgcg ccgcttcaat
     3301 gagctgcagt actgggtggc caccgagctg tgtctctgcc ccgtgcccgg cccccgggcc
     3361 cagctgctca ggaagttcat taagctggcg gcccacctca aggagcagaa gaatctcaat
     3421 tccttctttg ccgtcatgtt tggcctcagc aactcggcca tcagccacct agcccacacc
     3481 tgggagcggc tgccccacaa agtccggaag ctgtactccg ccctcgagag gctgctggac
     3541 ccctcatgga accaccgggt ataccgactg gccctcgcca agctctcccc tcctgtcatc
     3601 cccttcatgc cccttcttct caaagacatg accttcattc atgagggaaa ccacacacta
     3661 gtggagaatc tcatcaactt tgagaagatg agaatgatgg ccagagtcgc gcggatgctg
     3721 caccactgcc gaagccacaa ccctgtgcct ctctcaccac tcagaagccg agtttcccac
     3781 ctccacgagg acagccaggt ggcgaggatt tccacatgct cggagcagtc cctgagcacc
     3841 cggagtccag ccagcacctg ggcttatgtc cagcagctga aggtcattga caaccagcgg
     3901 gaactctccc gcctctcccg agagctggag ccagagctca tgcccgacaa ccactacctg
     3961 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4021 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggaggtacc
     4081 ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4201 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     4261 tggcccaccc tcgtgaccac cctgggctac ggccttcagt gcttcgcccg ctaccccgac
     4321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4381 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4501 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     4561 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     4621 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgtaagaa
     4681 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct
     4741 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     4801 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4861 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4921 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4981 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5041 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5101 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5161 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5221 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5281 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5341 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5401 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5461 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5521 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5581 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5641 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5701 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5761 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5821 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5881 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5941 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6001 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6061 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6121 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6181 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6241 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6301 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6361 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6421 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6481 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6541 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6601 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6661 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6721 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6781 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6841 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6901 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6961 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7021 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7081 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7141 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7201 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7861 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8281 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9181 acgtc
//
