LOCUS       sequence-364333-        8991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and 
            acceptor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke 
            M, Rangamani P, Taylor SS, Mehta S, Zhang J
  TITLE     Phase Separation of a PKA Regulatory Subunit Controls cAMP 
            Compartmentation and Oncogenic Signaling.
  JOURNAL   Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 
            10.1016/j.cell.2020.07.043. Epub 2020 Aug 25.
  PUBMED    32846158
REFERENCE   2  (bases 1 to 8991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07.043"; journalName: "Cell"; date: 
            "2020-09-17- 17"; volume: "182"; issue: "6"; pages: "1531-1544.e15"
COMMENT     Sequence Label: pcDNA3.1(+)-R/G-ICUE
FEATURES             Location/Qualifiers
     source          1..8991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     639..659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..752
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        733..751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             789..1499
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RITVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREVAV
                     AKFAGLGGGMDELYK"
     CDS             3705..4346
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     CDS             4347..4394
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     primer_bind     complement(4453..4470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4459..4683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4729..5156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4816..4835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5025..5046
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5165..5185)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5170..5499
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5350..5485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5412..5431
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5566..6360
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5620..5639)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6230..6249
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6534..6655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6571..6590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6625..6644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6704..6720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6704..6720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6717..6739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6728..6744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6752..6758,6759..6776,6777..6782))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6797..6818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6935..6952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7106..7694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7186..7205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7865..8656,8657..8725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8488..8507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8726..8830)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8905..8924)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      721 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt
      781 aagcttggat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg
      841 tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc
      901 cgtacatggg aactcaaacc atgaggatca cagtcatcga gggaggaccc ctgccatttg
      961 cctttgacat tcttgccacg tcgttcatgt atggcagccg tacttttatc aagtacccga
     1021 aaggcattcc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta
     1081 cgagatacga agatggtgga gtcgtcaccg tcatgcagga caccagcctt gaggatggct
     1141 gtctcgttta ccacgtccaa gtcagagggg taaactttcc ctccaatggt cccgtgatgc
     1201 agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc
     1261 tgaggggata cactcatatg gcactgaaag ttgatggtgg tggccatctg tcttgctctt
     1321 tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtatccatg
     1381 ccgttgatca ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc gtagtacaac
     1441 gcgaagtcgc agttgccaag ttcgccgggc ttggtggtgg gatggacgag ctgtacaagg
     1501 ctggtacccc cgtgggaact catgagatgg aggaggagtt ggccgaagct gtggccctgc
     1561 tctcccagcg ggggcctgac gccctgctca ctgtggcact tcgaaagccc ccaggtcagc
     1621 gcacggatga agagctggac ctcatctttg aggagctgct gcacatcaag gctgtggccc
     1681 acctctccaa ctcggtgaag cgagaattag cggctgttct gctctttgaa ccacacagca
     1741 aggcagggac cgtgttgttc agccaggggg acaagggcac ttcgtggtac attatctgga
     1801 agggatctgt caacgtggtg acccatggca aggggctggt gaccaccctg catgagggag
     1861 atgattttgg agaactggct ctggtgaatg atgcaccccg ggcagccacc atcatcctgc
     1921 gagaagacaa ctgtcatttc ctgcgtgtgg acaagcagga cttcaaccgt atcatcaagg
     1981 atgtggaggc aaagaccatg cggctggaag aacatggcaa agtggtgctg gtgctggaga
     2041 gagcctctca gggcgccggc ccttcccgac ccccaacccc aggcaggaac cggtatacag
     2101 tgatgtctgg caccccagag aagatcctag agcttctgtt ggaggccatg ggaccagatt
     2161 ccagtgctca tgaccaaaca gagacattcc tcagcgactt cctcctgacc cacagggtct
     2221 tcatgcccag cgcccaactc tgcgctgccc ttctgcacca cttccatgtg gagcctgcgg
     2281 gtggcagcga gcaggagcgc agcacctacg tctgcaacaa gaggcagcag atcttgcggc
     2341 tggtcagcca gtgggtggcc ctgtatggct ccatgctcca cactgaccct gtggccacca
     2401 gcttcctcca gaaactctca gacctggtgg gcagggacac ccgactcagc aacctgctga
     2461 gggagcagtg gccagagagg cggcgatgcc acaggttgga gaatggctgt gggaatgcat
     2521 ctcctcagat gaaggcccgg aacttgcctg tttggctccc caaccaggac gagccccttc
     2581 ctggcagcag ctgtgccatc caagttgggg ataaagtccc ctatgacatc tgccggccag
     2641 accactcagt gttgaccctg cagctgcctg tgacagcctc cgtgagagag gtgatggcag
     2701 cgttggccca ggaggatggc tggaccaagg ggcaggtgct ggtgaaggtc aattctgcag
     2761 gtgatgccat tggcctgcag ccagatgccc gtggtgtggc cacatctctg gggctcaatg
     2821 agcgtctctt tgttgtcaac ccacaggaag cgcatgagct gatcccacac cctgaccagc
     2881 tggggcccac tgtgggctct gctgaggggc tggacctggt gagtgccaag gacctggcag
     2941 gccagctgac ggaccacgac tggagcctct tcaacagtat ccaccaggtg gagctgatcc
     3001 actatgtgct gggcccccag catctgcggg atgtcaccac cgccaacctg gagcgcttca
     3061 tgcgccgctt caatgagctg cagtactggg tggccaccga gctgtgtctc tgccccgtgc
     3121 ccggcccccg ggcccagctg ctcaggaagt tcattaagct ggcggcccac ctcaaggagc
     3181 agaagaatct caattccttc tttgccgtca tgtttggcct cagcaactcg gccatcagcc
     3241 acctagccca cacctgggag cggctgcccc acaaagtccg gaagctgtac tccgccctcg
     3301 agaggctgct ggacccctca tggaaccacc gggtataccg actggccctc gccaagctct
     3361 cccctcctgt catccccttc atgccccttc ttctcaaaga catgaccttc attcatgagg
     3421 gaaaccacac actagtggag aatctcatca actttgagaa gatgagaatg atggccagag
     3481 tcgcgcggat gctgcaccac tgccgaagcc acaaccctgt gcctctctca ccactcagaa
     3541 gccgagtttc ccacctccac gaggacagcc aggtggcgag gatttccaca tgctcggagc
     3601 agtccctgag cacccggagt ccagccagca cctgggctta tgtccagcag ctgaaggtca
     3661 ttgacaacca gcgggaactc tcccgcctct cccgagagct ggagatgtcc aaaggagaag
     3721 aactgtttac cggtgttgtg ccaattttgg ttgaactcga tggtgatgtc aacggacata
     3781 agttctcagt gagaggcgaa ggagaaggtg acgccaccat tggaaaattg actcttaaat
     3841 tcatctgtac tactggtaaa cttcctgtac catggccgac tctcgtaaca acgcttacgt
     3901 acggagttca gtgcttttcg agatacccag accatatgaa aagacatgac ttttttaagt
     3961 cggctatgcc tgaaggttac gtgcaagaaa gaacaatttc gttcaaagat gatggaaaat
     4021 ataaaactag agcagttgtt aaatttgaag gagatacttt ggttaaccgc attgaactga
     4081 aaggaacaga ttttaaagaa gatggtaata ttcttggaca caaactcgaa tacaatttta
     4141 atagtcataa cgtatacatc actgctgata agcaaaagaa cggaattaaa gcgaatttca
     4201 cagtacgcca taatgtagaa gatggcagtg ttcaacttgc cgaccattac caacaaaaca
     4261 cccctattgg agacggtccg gtacttcttc ctgataatca ctacctctca acacaaacag
     4321 tcctgagcaa agatccaaat gaaaaacgtg accacatggt ccttcatgag tatgtaaatg
     4381 ctgctgggat tacacatggc atggatgagc tctacaaatg atctagaggg cccgtttaaa
     4441 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4501 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4561 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4621 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4681 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     4741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4921 acctcgaccc caaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4981 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5041 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5101 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     5161 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5221 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5341 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5401 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     5461 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttatata
     5521 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     5581 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5641 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5701 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5761 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5821 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5881 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5941 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6001 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6061 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     6121 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6181 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6241 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6301 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6361 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6421 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6481 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6661 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6781 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6901 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8821 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8881 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8941 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa t
//
