LOCUS       sequence-364339-        5253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF808 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF808
FEATURES             Location/Qualifiers
     source          1..5253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2810..2909)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2926..2945)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2927..2945)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3068..3087)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3079..3888
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3146..3165)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4035..4623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4524..4543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4777..4794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4953..4980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5072..5158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5207..5229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5221..5238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5222..5238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgctgagaga ggaggccgct
      121 cagaagagaa agggcaagga gagcggcatg gccctgcccc agggcagact gactttcaga
      181 gacgtggcta tcgaattcag cctggccgag tggaagttcc tgaaccccgc ccagagagcc
      241 ctgtacagag aggtgatgct ggaaaactac agaaacctgg aggccgtgga catcagcagc
      301 aagcacatga tgaaagaggt gctgagcacc ggccagggca acagggaggt gatacatacc
      361 ggcaccctgc agaggcacca gagctaccac atcggcgact tctgcttcca ggagatcgag
      421 aaggagatcc acaacatcga gttccagtgc caggaggacg agaggaacgg ccacgaggcc
      481 cctaccacca agatcaagaa actgaccggc tctacagacc agcacgacca cagacacgcc
      541 ggcaacaagc ctatcaagga ccagctgggc agcagcttct acagccacct ccccgagctg
      601 cacatcttcc agatcaaggg cgagatcgcc aatcagctgg agaagtccac cagcgacgcc
      661 agcagcgtga gcaccagcca gagaatctcc tgcagacccc agatccatat ctccaacaac
      721 tacggaaaca accccctgaa cagtagcctg ctcccccaga agcaggaggt gcacatgaga
      781 gagaagagct tcccctgcaa cgagtccggc aaggcattta actgcagctc cctgctgaga
      841 aagcaccaga tcccccacct gggcgacaag cagtacaagt gcgatgtgtg tggaaagctg
      901 ttcaaccaca aacagtacct ggcttgccat agaagatgtc acactgggga aaagccctac
      961 aaatgcaagg agtgtggcaa gtccttcagc tacaagtcca gcctgacctg ccaccacagg
     1021 ctgcacaccg gcgtgaagcc atacaagtgc aacgagtgcg gcaaggtctt tagacagaac
     1081 tccgccctgg tgatccataa agccatccat accggggaga aaccttataa gtgtaatgaa
     1141 tgcgggaaag cttttaacca acagtcccac ctgagcagac accagaggct gcatacaggc
     1201 gttaagcctt ataaatgtaa gatctgcgaa aaggcctttg cctgccactc ctatctggcc
     1261 aaccacacac ggatccattc tggcgaaaag acctacaagt gcaacgaatg tgggaaagcc
     1321 tttaaccacc agtcctccct ggctaggcac cacatcctgc ataccggtga aaaaccatat
     1381 aaatgtgagg agtgcgacaa ggttttctcc cagaagtcta ccctggaaag acacaagaga
     1441 atccacactg gtgagaagcc atataagtgc aaagtgtgcg atacagcctt cacctgtaac
     1501 agccagctcg ctagacacag aagaattcat accggagaaa aaacctacaa atgcaacgag
     1561 tgcagaaaga ctttctctag aaggtcaagc ctgctgtgcc ataggagact gcacagcggc
     1621 gaaaaacctt acaaatgcaa ccaatgtggc aacacattca gacacagggc ctctctcgtg
     1681 taccacagaa ggcttcacac cctcgagaaa tcctacaaat gcaccgtgtg caacaaggtg
     1741 ttcatgagaa acagcgtgct ggccgtgcac acccggatcc acaccgctaa gaaaccatac
     1801 aaatgtaacg aatgcggaaa ggccttcaac cagcagagcc acttgtccag gcacaggagg
     1861 ctccacacag gagagaagcc ctataagtgc gaagcctgcg ataaagtgtt cggccagaag
     1921 agcgccctgg agagccacaa gaggattcac accggagaga aaccataccg ctgccaggtg
     1981 tgcgacacag cattcacctg gaacagtcag ctggcccggc acacaaggat ccatactggc
     2041 gagaaaactt acaagtgcaa tgagtgcggg aagaccttta gctacaaatc tagcctggtc
     2101 tggcacagac gcctgcacgg cggggaaaaa agttacaagt gcaaggtgtg tgacaaggct
     2161 ttcgtgtgca gaagctacgt ggccaagcac accagaatcc attccggcat gaaaccctat
     2221 aaatgcaatg aatgtagcaa gaccttcagc aacaggagca gcctggtgtg tcaccggcgc
     2281 atccatagcg gtgagaaacc gtataaatgt tccgagtgct ccaagacatt cagccagaag
     2341 gccaccctgc tctgccacag acgactgcac tctggagaaa agccttacaa gtgtaacgac
     2401 tgcggcaaca ccttcagaca ttggagctcc ctggtgtatc acaggcgcct tcacacaggg
     2461 gaaaagagct ataagtgcac cgtctgtgac aaagcattcg tgaggaacag ctacctggcc
     2521 agacacatca gaatacacac cgccgagaaa ccctacaaat gtaatgagtg tgggaaggca
     2581 ttcaacgagc agtcccatct gagcaggcat caccgcatcc acacgggcga gaagccgtac
     2641 aaatgcgagg cctgcgacaa agtgtttagc aggaagagcc acctgaagag acaccggatc
     2701 attcatactg gtgaaaagcc gtataagtgc aacgagtgtg gtaaagcctt cagcgacaga
     2761 agcaccctga tccaccacca ggccatccac ggcatcggca agttcgacta cccaactttc
     2821 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2881 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2941 tattacatgg tcatagctgt ttcttggcag ctctggcccg tgtctcaaaa tctctgatgt
     3001 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     3061 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     3121 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     3181 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     3241 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     3301 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     3361 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     3421 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     3481 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     3541 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3601 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3661 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3721 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3781 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3841 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3901 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3961 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     4021 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4081 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4141 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4201 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4261 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4321 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4381 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4441 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4501 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4561 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4621 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4681 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4741 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4801 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4861 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4921 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4981 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     5041 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     5101 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     5161 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     5221 tgtaaaacga cggccagtct taagctcggg ccc
//
