LOCUS       sequence-364344-       10279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supe...
FEATURES             Location/Qualifiers
     source          1..10279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          667..1683
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1607..1629
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1691..1710
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1740..1773)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2307..2372
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2502..2522
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2947..3081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2984..3003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3038..3057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3123..3156)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             3199..3894
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     primer_bind     complement(4098..4115)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4325..4344
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4497..5114,5115..5120,5121..5156,5157..5159,5160..5195,
                     5196..5198,5199..5234,5235..5243)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5330..5350)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5748..5759)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5888..5907)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5953..6008
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6007..6026)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6392..6980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6472..6491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7309..7356
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7381..7400
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7588..8382)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7588..8382)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7699..7718)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8309..8328
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(8481..8500)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        8579..8770
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8727..8747
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8830..9621,9622..9690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9453..9472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9691..9795)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9822..10277
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9909..9928)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10119..10140
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
      421 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
      481 gtgcagcgat gggggcgggg gggggggggg sgcgcgccrg ggggggsggg gsggggsggg
      541 gggsggggsg gggsgrggsg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
      601 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      661 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      721 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      781 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
      841 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
      901 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
      961 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     1021 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     1081 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     1141 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     1201 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     1261 ggggcggggc cgcctcgggc cggggrgggc tcgggggagg ggcgcggcgg cccccggagc
     1321 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     1381 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     1441 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     1501 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     1561 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     1621 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     1681 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctag
     1741 aagttcctat actttctaga gaataggaac ttcattaagg gttccggatc ctcggggaca
     1801 ccaaatatgg cgatctcggc cttttcgttt cttggagctg ggacatgttt gccatcgatc
     1861 catctaccac cagaacggcc gttagatctg ctgccaccgt tgtttccacc gaagaaacca
     1921 ccgttgccgt aaccaccacg acggttgttg ctaaagaagc tgccaccgcc acggccaccg
     1981 ttgtagccgc cgttgttgtt attgtagttg ctactgttat ttctggcact tcttggtttt
     2041 cctcttaagt gaggaggaac ataaccattc tcgttgttgt cgttgatgct taaattttgc
     2101 acttgttcgc tcagttcagc cataatatga aatgcttttc ttgttgttct tacggaatac
     2161 cacttgccac ctatcaccac aactaacttt ttcccgttcc tccatctctt ttatattttt
     2221 tttctcgagg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     2281 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     2341 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     2401 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     2461 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     2521 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     2581 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     2641 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     2701 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     2761 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     2821 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     2881 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     2941 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3061 gtatcttatc atgtctggat ctgacatggt aagtaagctt gggctgcagg tcgagggacc
     3121 tagaagttcc tatactttct agagaatagg aacttcatgt cgacggtacc gcgggcccgg
     3181 gatccaccgg tcgccaccat gagcgagctg atcaaggaga acatgcacat gaagctgtac
     3241 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc
     3301 tacgagggca cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc
     3361 ttcgacatcc tggctaccag cttcatgtac ggcagcaaag ccttcatcaa ccacacccag
     3421 ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga gagaatcacc
     3481 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcttcca gaacggctgc
     3541 atcatctaca acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag
     3601 aagaaaacac gcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg
     3661 agaggccaca gccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccttc
     3721 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cttccacttc
     3781 gtggaccaca gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac
     3841 gagatggctg tggccaagta ctgcgacctc cctagcaaac tggggcacag atgatgctct
     3901 agatcgagtt aattaaggat tccgcccccc cccctctccc tccccccccc ctaacgttac
     3961 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     4021 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     4081 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     4141 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     4201 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     4261 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     4321 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     4381 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     4441 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt tcctttgaaa aacacgatgt
     4501 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat gaagtcggta
     4561 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     4621 actggcacgt gaagaacaag cgggccctgc tcgatgccct ggcaatcgag atgctggaca
     4681 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     4741 acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     4801 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     4861 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     4921 tgggctgcgt attggaggat caggagcatc aagtagcaaa agaggaaaga gagacaccta
     4981 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac catcagggag
     5041 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     5101 agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag
     5161 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     5221 accttgacat gctccccggg taactaagta agcggccgcg aattcgccct tcggccgctc
     5281 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5341 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5401 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5461 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5521 ggggcattgc caccacctgt cagctccttt ccggaacttt cgctttcccc ctccctattg
     5581 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5641 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5701 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5761 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5821 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcagcgg ccgcggcgac
     5881 tcgaggatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     5941 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     6001 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     6061 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     6121 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     6181 ccttgacttg aggttagatt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     6241 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     6301 agtcatagct gtccctcttc tcttatgaag ccatgtgagc aaaaggccag caaaaggcca
     6361 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6421 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6481 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6541 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6601 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6661 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6721 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6781 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6841 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6901 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6961 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7021 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7081 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg
     7141 aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac
     7201 ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg
     7261 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
     7321 caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta
     7381 ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga
     7441 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
     7501 tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt
     7561 cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
     7621 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     7681 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     7741 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     7801 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt
     7861 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     7921 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     7981 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     8041 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     8101 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     8161 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc
     8221 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     8281 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     8341 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
     8401 tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     8461 ggaatagctc agaggccggg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     8521 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     8581 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     8641 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     8701 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt
     8761 tccgcctcag gactcttcct ttttcaataa atcaatctaa agtatatatg agtaaacttg
     8821 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8881 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8941 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9001 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9061 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9121 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9181 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9241 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9301 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9361 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9421 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9481 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9541 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9601 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9661 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9721 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9781 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     9841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10021 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10141 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    10201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    10261 attaacgctt acaatttac
//
