LOCUS       sequence-364357-        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a fusion of the transmembrane protein TVA and the red 
            fluorescent protein mCherry under UAS control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Satou C, Neve RL, Oyibo HK, Zmarz P, Huang KH, Arn Bouldoires E, 
            Mori T, Higashijima SI, Keller GB, Friedrich RW
  TITLE     A viral toolbox for conditional and transneuronal gene expression in
            zebrafish.
  JOURNAL   Elife. 2022 Jul 22;11. pii: 77153. doi: 10.7554/eLife.77153.
  PUBMED    35866706
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-07-22"; volume: "11. pii: 77153. doi"; pages: " 
            10.7554/eLife.77153"
COMMENT     Sequence Label: UAS:TVA-mCherry
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             445..1149
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(587..605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     855..874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gccaccatgg cgcggctgct gcccgcgctg ctgctgctgc tgctgcccgg taacgtgacc
       61 ggtaacgggt ccggtaacgg ttctttgtcc cgttgccccc ccggtcagtt ccgctgctcg
      121 gagccgcccg gtgcccacgg ggagtgttac ccgcaggact ggctgtgcga cggacacccc
      181 gactgcgacg acgggcggga cgagtggggc tgcgggacca gcgcgatccc cgcggtgccc
      241 acggacaacg gcacagaggc tcccactgcc cctgctcctg gacgtgctct gccagccagg
      301 aatcacggcc gcatgtggat gctgatcact gcagtgctcc tgtgctgcct ggtagctgtg
      361 ggtggtatcg ctgcatgggg gaagtccaaa gcaaaaagca ggtctgacat cttcagtctt
      421 gaaagcgcat ccaagggggg tggggtgagc aagggcgagg aggataacat ggccatcatc
      481 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      541 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      601 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      661 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      721 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      781 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      841 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      901 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      961 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1021 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     1081 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1141 ctgtacaag
//
