LOCUS       sequence-364376-        7446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep 
            expression construct with endogenous AAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Landegger LD, Pan B, Askew C, Wassmer SJ, Gluck SD, Galvin A, Taylor
            R, Forge A, Stankovic KM, Holt JR, Vandenberghe LH
  TITLE     A synthetic AAV vector enables safe and efficient gene transfer to 
            the mammalian inner ear.
  JOURNAL   Nat Biotechnol. 2017 Mar;35(3):280-284. doi: 10.1038/nbt.3781. Epub 
            2017 Feb 6.
  PUBMED    28165475
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3781"; 
            journalName: "Nat Biotechnol"; date: "2017-03"; volume: "35"; issue:
            "3"; pages: "280-284"
COMMENT     Sequence Label: pAnc80L65AAP
FEATURES             Location/Qualifiers
     source          1..7446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     395..414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     648..665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     810..826
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     810..826
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1304..1315)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5316..5332)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5317..5333)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5367..5384)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5367..5383)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5376..5398)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5525..5980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5612..5631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5822..5843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     terminator      6016..6043
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(6249..6268)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6260..7075
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6333..6352)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7094..7121
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      join(7352..7446,1..494)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
       61 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      601 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      661 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      721 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      781 ttaggcaccc caggctgtgg aatttcacac aggaaacagc tatgaccatg attacgccaa
      841 gcgcgcgaac aaaagctgga gctccaccgc ggtggcggcc gctctagtcg agcggccgcc
      901 agtgtgatgg atatctgcag aattcggctt ggcggctgcg cgttcaaacc tcccgcttca
      961 aaatggagac cctgcgtgct cactcgggct taaataccca gcgtgaccac atggtgtcgc
     1021 aaaatgtcgc aaaacactca cgtgacctct aatacaggac ctctagagca tggaaactag
     1081 ataagaaaga aatacgcaga gaccaaagtt caactgaaac gaattaaacg gtttattgat
     1141 taacaagcaa actagtttac agattacggg tgaggtaacg ggtgccgatg gggcgaggct
     1201 cagaataaac gccatttgtg tcaacagcaa agtccacatt tgtagatttg ttgtagttgg
     1261 aagtgtattg aatctctggg ttccagcgtt tgctgttttc tttctgcagc tcccattcaa
     1321 tttccacgct gacctgtccg gtgctgtact gcgtgatgaa cgacgcaaac ttagctggac
     1381 tgaaggtagt tggaggattc gcgggaacag gtgtattctt aatcaggatc tgaggaggcg
     1441 ggtgtttcag tccaaagcct cccatcagcg gcgagggatg aaagtgtccg tccgtgtgag
     1501 gaatcttggc ccagatagga ccctgcaggt acacgtcccg gtcctgccag accatgccag
     1561 gtaaggctcc ttgactgttg acggtccctg tagcaggagc ggtgttggcc gattgcaggt
     1621 tagtggccac cgtgccgtac tcttctgtgg ccactgggtt ggtggtttta atttcttcct
     1681 cgttggttat cataacgttg tcaaggtcca cgttgctatt tccagctccc tgtttcccaa
     1741 atattaagac tccgctcatc ggaaaaaatt tgtcttcgtc gtccttgtgg gttgccatag
     1801 cgggaccggg atttaccaga gagtctctgc cattcagatg atacttggtg gcaccggtcc
     1861 aggcaaagtt gctgttgtta ttttgattgg ttgtcttgga gacgcgttgc tgccggtagc
     1921 agggcccggg tagccagttt ttggcctgat tcgccatgct actaggcccg gcctgagaaa
     1981 attgcaacgt ccgatttcct gcggtaccac tcgtggtctg agtccgagac aggtagtaca
     2041 ggtactggtc gatgaggggg ttcatcagcc ggtccaggct ttggctgtgc gcgtagctgc
     2101 tgtgaaaagg cacgtcctca aacgtgtagc tgaactgaaa gttgttgccc gttctcagca
     2161 tttgagaagg aaagtattcc aggcagtaga aggaggaacg gcccacggcc tgactgccat
     2221 tgttcagagt caggtacccg tactgaggaa tcatgaagac gtccgccggg aacggaggca
     2281 ggcagccctg gtgcgcagag ccgaggacgt acgggagctg gtattccgag tccgtaaaga
     2341 cctgaaccgt gctggtaagg ttattggcga tggtcgtggt gccatcattc gtcgtgacct
     2401 ccttgacctg gatgttgaag agcttgaagt tgagcttctt gggccggaat ccccagttgt
     2461 tgttgatgag tcgctgccag tcacgtggtg agaagtggca gtggaatctg ttaaagtcaa
     2521 aataccccca gggggtgctg tagccgaagt aggtgttgtc gttggtgctg cctcccgatt
     2581 ggctggagat ttgcttgtag aggtggttgt tgtaggtggg gagggcccag gttcgggtgc
     2641 tggtggtgat gactctgtcg cccagccatg tggaatcgca atgccaattt cctgaggcgt
     2701 tacccactcc gtcggcgcct tcgttattgt ctgccattgg agcgccaccg cctgcagcca
     2761 ttgtattaga tcccacacca gagggggctg cggggggttc tccgagtggt tgagggtcgg
     2821 gcactgactc tgagtcgcca gtctgcccaa agttgagtct ctttctcgcg ggctgctggc
     2881 ctttcttgcc gatgcccgaa gaggagtctg gttcctgggg tgattgctct accggtctct
     2941 tctttccagg agccgtctta gcgccttcct caaccagacc gagaggttcg agaacccgct
     3001 tcttggcctg gaagactgct cgcccgaggt tgcccccaaa agacgtatct tcttgcagac
     3061 gctcctgaaa ctcggcgtcg gcgtggttat accgcaggta cggattgtca cccgctttga
     3121 gctgctggtc gtaggccttg tcgtgctcga gggccgctgc gtccgccgcg ttgacgggct
     3181 cccccttgtc gagtccgttg aagggtccga ggtacttgta gccaggaagc accagacccc
     3241 ggccgtcgtc ctgcttttgc tggttggctt tgggtttcgg ggctccaggt ttcaagtccc
     3301 accactcgcg aatgccctca gagaggttgt cctcgagcca atctggaaga taaccatcgg
     3361 cagccatacc tgatttaaat catttattgt tcaaagatgc agtcatccaa atccacattg
     3421 accagatcgc aggcagtgca agcgtctggc acctttccca tgatatgatg aatgtagcac
     3481 agtttctgat acgccttttt gacgacagaa acgggttgag attctgacac gggaaagcac
     3541 tctaaacagt ctttctgtcc gtgagtgaag cagatatttg aattctgatt cattctctcg
     3601 cattgtctgc agggaaacag catcagattc atgcccacgt gacgagaaca tttgttttgg
     3661 tacctgtctg cgtagttgat cgaagcttcc gcgtctgacg tcgatggctg cgcaactgac
     3721 tcgcgcaccc gtttgggctc acttatatct gcgtcactgg gggcgggtct tttcttggct
     3781 ccaccctttt tgacgtagaa ttcatgctcc acctcaacca cgtgatcctt tgcccaccgg
     3841 aaaaagtctt tgacttcctg cttggtgacc ttcccaaagt catgatccag acggcgggtg
     3901 agttcaaatt tgaacatccg gtcttgcaac ggctgctggt gttcgaaggt cgttgagttc
     3961 ccgtcaatca cggcgcacat gttggtgttg gaggtgacga tcacgggagt cgggtctatc
     4021 tgggccgagg acttgcattt ctggtccacg cgcaccttgc ttcctccgag aatggctttg
     4081 gccgactcca cgaccttggc ggtcatcttc ccctcctccc accagatcac catcttgtcg
     4141 acacagtcgt tgaagggaaa gttctcattg gtccagttta cgcacccgta gaagggcaca
     4201 gtgtgggcta tggcctccgc gatgttggtc ttcccggtag ttgcaggccc aaacagccag
     4261 atggtgttcc tcttgccgaa ctttttcgtg gcccatccca gaaagacgga agccgcatat
     4321 tggggatcgt acccgtttag ttccaaaatt ttataaatcc gattgctgga aatgtcctcc
     4381 acgggctgct ggcccaccag gtagtcgggg gcggttttag tcaggctcat aatctttccc
     4441 gcattgtcca aggcagcctt gatttgggac cgcgagttgg aggccgcatt gaaggagatg
     4501 tatgaggcct ggtcctcctg gatccactgc ttctccgagg taatcccctt gtccacgagc
     4561 cacccgacca gctccatgta cctggctgaa gtttttgatc tgatcaccgg cgcatcagaa
     4621 ttgggattct gattctcttt gttctgctcc tgcgtctgcg acacgtgcgt cagatgctgc
     4681 gccaccaacc gtttacgctc cgtgagattc aaacaggcgc ttaaatactg ttccatatta
     4741 gtccacgccc actggagctc aggctgggtt ttggggagca agtaattggg gatgtagcac
     4801 tcatccacca ccttgttccc gcctccggcg ccatttctgg tctttgtgac cgcgaaccag
     4861 tttggcaaag tcggctcgat cccgcggtaa attctctgaa tcagtttttc gcgaatctga
     4921 ctcaggaaac gtcccaaaac catggatttc accccggtgg tttccacgag cacgtgcatg
     4981 tggaagtagc tctctccctt ctcaaattgc acaaagaaaa gagcctccgg ggccttactc
     5041 acacggcgcc attccgtcag aaagtcgcgc tgcagcttct cggccacggt caggggtgcc
     5101 tgctcaatca gattcagatc catgtcagaa tctggcggca actcccattc cttctcggcc
     5161 acccagttca caaagctgtc agaaatgccg ggcagatgct cgtcaaggtc gctggggacc
     5221 ttaatcacaa tctcgtaaaa ccccggcatg gcggctgcgc gttcaaacct cccgcttcaa
     5281 aatggagacc ctgcgtgctc actcgggctt aaatacgata ccgtcgacct cgaggggggg
     5341 cccggtaccc aattcgccgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     5401 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5461 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5521 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5581 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5641 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5701 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5761 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5821 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5881 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5941 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     6001 atgtgccgtc acactggctc accttcgggt gggcctttct gcgtttatat actagagaga
     6061 gaatataaaa agccagatta ttaatccggc ttttttatta ttttactaga gctgatcctt
     6121 caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg
     6181 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     6241 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cccgtccgcg
     6301 cttaaactcc aacatggacg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     6361 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     6421 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtcc gtctcaactg
     6481 gctgacggag tttatgcctc tcccgaccat caagcatttt atccgtactc ctgatgatgc
     6541 gtggttactc accaccgcga ttcctgggaa aacagccttc caggtattag aagaatatcc
     6601 tgattcaggt gaaaatattg ttgatgcgct ggccgtgttc ctgcgccggt tacattcgat
     6661 tcctgtttgt aattgtcctt ttaacagcga tcgtgtattt cgtcttgctc aggcgcaatc
     6721 acgcatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     6781 tgttgaacaa gtctggaaag aaatgcacaa gctcttgcca ttctcaccgg attcagtcgt
     6841 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     6901 tattgatgtt ggacgggtcg gaatcgcaga ccgttaccag gaccttgcca ttctttggaa
     6961 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     7021 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaataata
     7081 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
     7141 atataaaaag ccagattatt aatccggctt ttttattatt tctgtcagac caagtttact
     7201 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7261 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7321 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7381 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7441 taccaa
//
