LOCUS       sequence-364383-       18916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fungal AMA1 plasmid with sp-Cas9, ribozymes based ""plug-and-play"" 
            sgRNA transcription unit, Terbinafine and Phleomycin selection 
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18916)
  AUTHORS   Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen 
            AJM
  TITLE     CRISPR-based transcriptional activation tool for silent genes in 
            filamentous fungi.
  JOURNAL   Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3.
  PUBMED    33441979
REFERENCE   2  (bases 1 to 18916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-01-13"; volume: "11(1):1118. doi"; pages: " 
            10.1038/s41598-020-80864-3"
COMMENT     Sequence Label: pLM-AMA15.0 Cas9
FEATURES             Location/Qualifiers
     source          1..18916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1302..1417
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             4633..8736
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4633..8736
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8749..8769
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8773..8793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     ncRNA           complement(9394..9461)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        complement(9466..9541)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(9635..9958)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     9640..9659
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9868..9884
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9885..9941
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9954..9970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9978..9994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10002..10008,10009..10026,10027..10032))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10047..10068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     intron          10140..10255
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     primer_bind     complement(10734..10753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10735..10753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10763..10779)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10921..11376
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11008..11027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11218..11239
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11402..11506
                     /gene="bla"
                     /label=AmpR promoter
     promoter        11826..11928
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11929..12588
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11995..12014)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      12977..13565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13466..13485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13719..13736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13853..13874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13889..13894,13895..13912,13913..13919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13927..13943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13951..13967
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13986..14006
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13988..14006
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             14798..15172
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      15257..15504
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(15273..15291)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 actttggcct caatctagaa tctatctagt tgtcaactag actgtggtat cattgtcttt
       61 tattttccta gtcctggaac tagcttctaa ctagtctccc taatatgtgg ctgtcttgtt
      121 tttttttttt gtttccctac ccggatatct agtccccttc taggttctgt taacctctcg
      181 ggctctgatt tagtttaacg caaacctgag attagtttct aactagtctc taggttttct
      241 atccaccttt aattgtaata ataaatacaa gcaacgttta tacgtcaaaa gcatttataa
      301 acttttaccc taaagtagct tgcttgtgtg tttagtttat aattagtctc ttattaattt
      361 gatgtaggta agcccgccac aaatatatat ttttaacaag ataccgtgga aaaacttcgt
      421 gctatcacaa aacagtatac aaaaaataag ctatcgaatt cctgcagccc cagatcatcc
      481 tgtcttcagt cttaagaatt cgagctcgga gtggatcccc cgggctgcag gaattcgagc
      541 tctgtacagt gaccggtgac tctttctggc atgcggagag acggacggac gcagagagaa
      601 gggctgagta ataagcgcca ctgcgccaga cagctctggc ggctctgagg tgcagtggat
      661 gattattaat ccgggaccgg ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct
      721 cgttgaccaa gaatctattg catcatcgga gaatatggag cttcatcgaa tcaccggcag
      781 taagcgaagg agaatgtgaa gccaggggtg tatagccgtc ggcgaaatag catgccatta
      841 acctaggtac agaagtccaa ttgcttccga tctggtaaaa gattcacgag atagtacctt
      901 ctccgaagta ggtagagcga gtacccggcg cgtaagctcc ctaattggcc catccggcat
      961 ctgtagggcg tccaaatatc gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg
     1021 ccgctcagga gctggccagc ggcgcagacc gggaacacaa gctggcagtc gacccatccg
     1081 gtgctctgca ctcgacctgc tgaggtccct cagtccctgg taggcagctt tgccccgtct
     1141 gtccgcccgg tgtgtcggcg gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc
     1201 atattttcct gctctcccca ccagctgctc ttttcttttc tctttctttt cccatcttca
     1261 gtatattcat cttcccatcc aagaaccttt atttccccta agtaagtact ttgctacatc
     1321 catactccat ccttcccatc ccttattcct ttgaaccttt cagttcgagc tttcccactt
     1381 catcgcagct tgactaacag ctaccccgct tgagcagaca tcacaatgac cttgctcaat
     1441 ggccacgcct ccctctcccc ggccgctcaa cgccgcatcg aacaccatga agctgatatt
     1501 gtaattgtgg gtgccggagt cctcggctgt gcacttgcag ttgcactggg aaatcaaggg
     1561 cgcagtgtaa tcctgctgga gaagtcgttg gaagagccca accgtattgt tggtgaacta
     1621 ctccaaccgg gaggtgttca ggcgctcgag caactagggc tacgggattg tctggaagat
     1681 atcgatggca tcaatgtgaa gggatactgg gttacttact ttggggagcc ggttctcctc
     1741 gaatacccca agtccagccc aacctcgcct acaccgttgg gacgagcctt ccaccatggc
     1801 cgattcgtga tgaaactacg ggcagccgct ctgtcgtgcc ccaatgtcac ggtggtcgaa
     1861 accaaggtta ccggcctcat cacatcctca tatactcagg aagtccttgg agtcgagtgt
     1921 ataaccaagg atgtcaagga ctgctacttc ggtcaactca ccgtggccgc cgacggttat
     1981 aactccgttt tccgcaagga acaccaccag tatacaccca aacggcgctc aaagttccat
     2041 ggcttggagc tcatcgatgc taaactgccc gctcccaaca ctggtcatgt gcttctcagc
     2101 gataatccac ccgtacttat gtaccagatc ggcacccatg aaacccgaat ccttatcgat
     2161 atccctgata acctgcctgc ggcctcggtc aagaacggtg gtgtcaaggg ttacatgcga
     2221 aacaacatcc tgcccaagct cccagagggt gtccagcagt cgttctccga tgccctagag
     2281 aagggccagc tacggtcaat gccgaactcg ttcctgcctg cctcggccaa caagacaccc
     2341 ggcttgatga ttctgggcga tgcgcttaac atgcggcacc ctctcacagg tggaggcatg
     2401 actgtggcgc tcaacgatgt ttgtgtcatc cgcgaattgc tcagcccaga gcgcgtgccc
     2461 aacctctcca atacaggcct tgttctcgag cagctcgcag agttccactg gaaacgaaag
     2521 aactcatctt cggtcatcaa tatcctcgca caggctctct atgcgctctt cgctgcagac
     2581 agtacgcacg ctcactcttc aggttaattt gcaataacta actgttcatt caacagacta
     2641 ttacctcaaa gctcttcagc gtggctgttt ccgctacttc cagattggac cagttggcgg
     2701 ccctgtcggg ttgttagccg gtctaatcaa gaaacccctc gtccttgtca gccacttctt
     2761 ctccgttgcc ttcctcgcca tctgggtcca catatgcgat acccctctgt ccaagctatt
     2821 cctagcccca tactacgccg tcatgatatt gtacacagcc tctgtcgtga ttttgccata
     2881 catctggacc gagatctggt actgagcttc taataagtgt cagatagcaa tttgcacaag
     2941 aaatcaatac cagcaactgt aaataagcgc tgaagtgacc atgccatgct acgaaagagc
     3001 agaaaaaaac ctgccgtaga accgaagaga tatgacacgc ttccatctct caaaggaaga
     3061 atcccttcag ggttgcgttt ccagtctaga cacgtataac ggcacaagtg tctctcacca
     3121 aatgggttat atctcaaatg tgatctaagg atggaaagcc cagaatattg gctgggttga
     3181 tggctgcttc gagtgcagtc tcatgctgcc acaggtgact ctggatggcc ccataccact
     3241 caacccatgg tacgagcggt agcaagaatt caagcttgga ggagttatag acggtccggc
     3301 ataggtaagg agagattaga tatttcgact tcccgattcc ttagtctcct tagtctggaa
     3361 actgagaaag gcctgaactc gttcaatcta ttagctctaa tcacgtagtc tcaactagaa
     3421 tcctgaacag gcgccggaac cagtccaaat gcgtggagta cagcaagtcc agtcaaatca
     3481 cgattagaat ggccatatat aagcaaaaaa cccttggaca atacgtccga tcgacaccaa
     3541 agcaatctgg tacatcaccc ttcttatata cagggtagcc ggaattgtat tgactctata
     3601 cgaagccgca ggtttaccag cacagaggtg cgatagctac acgaatgtag agtgaaggca
     3661 ttacaaccga ttgctaagtg tcatagaggc taagcttcac ggctcaagga gtctgtggag
     3721 gatacctgat ataagaattg agagttatac tccggaggtt atgttagtac ctcaaaaatg
     3781 tgaagtacaa cgaaactcga acttcgaatg acaatagctt ggctggctgt tagtcaactg
     3841 aggtacaacg tcatcttgca attagttatg ttctttgcta gatagtgagc tgctgtaaac
     3901 ttgtagttct tagaaacatg tctgaagtct agagtcgaga cttgtgattg gtgtaaatta
     3961 gtgggggtcc ggagcggaat aacaatcgag gtcagcctcc tgggtctgtc agtaagctaa
     4021 ccacgaaagg acaagcgtag acgaggtcac ggggtactgg cctagccggc ttcggctagc
     4081 gcccacgaaa atagggcaac gtcgaagcga gagacacaca cggattccac gatcgccgac
     4141 gacaagcact caactccacg acaacccacg acaacccttg ccgctgcgtt cttggtgaag
     4201 ggctgcagga ttagaggtga gtattttatt ttctggctat tgtgatcttt ttcccccccg
     4261 gtttccgctg gttctatctt gtcgagcgag tcggtgcgcc ttcgatcggt ctgtcaaatt
     4321 tccatccgtc cactcgttca cccggtatcg atacaagagg gaatggagca ttacgacagg
     4381 ggagcagagg cagaactctc gatgttgcta ctttttttgc ttggaacaac ttccttgaaa
     4441 gattcttttc tgaatgcggc tgggaaactg cgaattggat atccagcgaa aggttaccga
     4501 cagactcacg gccatcgata ccactcagcg ggatagaatt gaaggcattc ggacattaga
     4561 tgatcagctt cgcttgtttc gactcgtgct aatcaattat ccctggacag tcccccacat
     4621 agacagcaaa caatggacaa gaagtattct atcggactgg atatcgggac taatagcgtc
     4681 gggtgggccg tgatcactga cgagtacaag gtgccctcta agaagttcaa ggtgctcggg
     4741 aacaccgacc ggcattccat caagaaaaat ctgatcggag ctctcctctt tgattcaggg
     4801 gaaaccgctg aagcaacccg cctcaagcgg actgctagac ggcggtacac caggaggaag
     4861 aaccggattt gttaccttca agagatattc tccaacgaaa tggcaaaggt cgacgacagc
     4921 ttcttccata ggctggaaga atcattcctc gtggaagagg ataagaagca tgaacggcat
     4981 cccatcttcg gtaatatcgt cgacgaggtg gcctatcacg agaaataccc aaccatctac
     5041 catcttcgca aaaagctggt ggactcaacc gacaaggcag acctccggct tatctacctg
     5101 gccctggccc acatgatcaa gttcagaggc cacttcctga tcgagggcga cctcaatcct
     5161 gacaatagcg atgtggataa actgttcatc cagctggtgc agacttacaa ccagctcttt
     5221 gaagagaacc ccatcaatgc aagcggagtc gatgccaagg ccattctgtc agcccggctg
     5281 tcaaagagcc gcagacttga gaatcttatc gctcagctgc cgggtgaaaa gaaaaatgga
     5341 ctgttcggga acctgattgc tctttcactt gggctgactc ccaatttcaa gtctaatttc
     5401 gacctggcag aggatgccaa gctgcaactg tccaaggaca cctatgatga cgatctcgac
     5461 aacctcctgg cccagatcgg tgaccaatac gccgaccttt tccttgctgc taagaatctt
     5521 tctgacgcca tcctgctgtc tgacattctc cgcgtgaaca ctgaaatcac caaggcccct
     5581 ctttcagctt caatgattaa gcggtatgat gagcaccacc aggacctgac cctgcttaag
     5641 gcactcgtcc ggcagcagct tccggagaag tacaaggaaa tcttctttga ccagtcaaag
     5701 aatggatacg ccggctacat cgacggaggt gcctcccaag aggaatttta taagtttatc
     5761 aaacctatcc ttgagaagat ggacggcacc gaagagctcc tcgtgaaact gaatcgggag
     5821 gatctgctgc ggaagcagcg cactttcgac aatgggagca ttccccacca gatccatctt
     5881 ggggagcttc acgccatcct tcggcgccaa gaggacttct acccctttct taaggacaac
     5941 agggagaaga ttgagaaaat tctcactttc cgcatcccct actacgtggg acccctcgcc
     6001 agaggaaata gccggtttgc ttggatgacc agaaagtcag aagaaactat cactccctgg
     6061 aacttcgaag aggtggtgga caagggagcc agcgctcagt cattcatcga acggatgact
     6121 aacttcgata agaacctccc caatgagaag gtcctgccga aacattccct gctctacgag
     6181 tactttaccg tgtacaacga gctgaccaag gtgaaatatg tcaccgaagg gatgaggaag
     6241 cccgcattcc tgtcaggcga acaaaagaag gcaattgtgg accttctgtt caagaccaat
     6301 agaaaggtga ccgtgaagca gctgaaggag gactatttca agaaaattga atgcttcgac
     6361 tctgtggaga ttagcggggt cgaagatcgg ttcaacgcaa gcctgggtac ttaccatgat
     6421 ctgcttaaga tcatcaagga caaggatttt ctggacaatg aggagaacga ggacatcctt
     6481 gaggacattg tcctgactct cactctgttc gaggaccggg aaatgatcga ggagaggctt
     6541 aagacctacg cccatctgtt cgacgataaa gtgatgaagc aacttaaacg gagaagatat
     6601 accggatggg gacgccttag ccgcaaactc atcaacggaa tccgggacaa acagagcgga
     6661 aagaccattc ttgatttcct taagagcgac ggattcgcta atcgcaactt catgcaactt
     6721 atccatgatg attccctgac ctttaaggag gacatccaga aggcccaagt gtctggacaa
     6781 ggtgactcac tgcacgagca tatcgcaaat ctggctggtt cacccgctat taagaagggt
     6841 attctccaga ccgtgaaagt cgtggacgag ctggtcaagg tgatgggtcg ccataaacca
     6901 gagaacattg tcatcgagat ggccagggaa aaccagacta cccagaaggg acagaagaac
     6961 agcagggagc ggatgaaaag aattgaggaa gggattaagg agctcgggtc acagatcctt
     7021 aaagagcacc cggtggaaaa cacccagctt cagaatgaga agctctatct gtactacctt
     7081 caaaatggac gcgatatgta tgtggaccaa gagcttgata tcaacaggct ctcagactac
     7141 gacgtggacc atatcgtccc tcagagcttc ctcaaagacg actcaattga caataaggtg
     7201 ctgactcgct cagacaagaa ccggggaaag tcagataacg tgccctcaga ggaagtcgtg
     7261 aaaaagatga agaactattg gcgccagctt ctgaacgcaa agctgatcac tcagcggaag
     7321 ttcgacaatc tcactaaggc tgagaggggc ggactgagcg aactggacaa agcaggattc
     7381 attaaacggc aacttgtgga gactcggcag attactaaac atgtcgccca aatccttgac
     7441 tcacgcatga ataccaagta cgacgaaaac gacaaactta tccgcgaggt gaaggtgatt
     7501 accctgaagt ccaagctggt cagcgatttc agaaaggact ttcaattcta caaagtgcgg
     7561 gagatcaata actatcatca tgctcatgac gcatatctga atgccgtggt gggaaccgcc
     7621 ctgatcaaga agtacccaaa gctggaaagc gagttcgtgt acggagacta caaggtctac
     7681 gacgtgcgca agatgattgc caaatctgag caggagatcg gaaaggccac cgcaaagtac
     7741 ttcttctaca gcaacatcat gaatttcttc aagaccgaaa tcacccttgc aaacggtgag
     7801 atccggaaga ggccgctcat cgagactaat ggggagactg gcgaaatcgt gtgggacaag
     7861 ggcagagatt tcgctaccgt gcgcaaagtg ctttctatgc ctcaagtgaa catcgtgaag
     7921 aaaaccgagg tgcaaaccgg aggcttttct aaggaatcaa tcctccccaa gcgcaactcc
     7981 gacaagctca ttgcaaggaa gaaggattgg gaccctaaga agtacggcgg attcgattca
     8041 ccaactgtgg cttattctgt cctggtcgtg gctaaggtgg aaaaaggaaa gtctaagaag
     8101 ctcaagagcg tgaaggaact gctgggtatc accattatgg agcgcagctc cttcgagaag
     8161 aacccaattg actttctcga agccaaaggt tacaaggaag tcaagaagga ccttatcatc
     8221 aagctcccaa agtatagcct gttcgaactg gagaatgggc ggaagcggat gctcgcctcc
     8281 gctggcgaac ttcagaaggg taatgagctg gctctcccct ccaagtacgt gaatttcctc
     8341 taccttgcaa gccattacga gaagctgaag gggagccccg aggacaacga gcaaaagcaa
     8401 ctgtttgtgg agcagcataa gcattatctg gacgagatca ttgagcagat ttccgagttt
     8461 tctaaacgcg tcattctcgc tgatgccaac ctcgataaag tccttagcgc atacaataag
     8521 cacagagaca aaccaattcg ggagcaggct gagaatatca tccacctgtt caccctcacc
     8581 aatcttggtg cccctgccgc attcaagtac ttcgacacca ccatcgaccg gaaacgctat
     8641 acctccacca aagaagtgct ggacgccacc ctcatccacc agagcatcac cggactttac
     8701 gaaactcgga ttgacctctc acagctcgga ggggatgagg gagctgatcc aaaaaagaag
     8761 agaaaggtag atccaaaaaa gaagagaaag gtataagctt acttctttat cggttctctc
     8821 ttacgacttt ttgaatggaa cgtttccttc ttctcaggcg ggcctatctt tgggccgaag
     8881 ctcttttcct tgtactgtag gacctggttg ataatgattc ccaaaaagac atccagcatg
     8941 tcagttactt gcattcgtca gtctatacaa aagcaatggt ttagagaaat tttgaacttt
     9001 atacatggtt ttatttgttg cttcacggcc gtaccttctg gaaatccacg gtaggagtgt
     9061 caatttgcgt ttttgataat ccttccaagg ttcttctcga agtagttgtt ctataattgc
     9121 ttcacagcta ccatggaaca tcccaagcac cgctactaga gccaagagcg gattcctcag
     9181 tctcgtacgt ctcttgacga ccgttgatct gcttgatctc gtctcccgaa aatgaaaata
     9241 gactctgcta agctattctt ctgcttcgcc ggagcctgaa gggcgtacta gggttgcgag
     9301 gtccaatgca ttaatgcatt gcagatgagc tgtatctgga agaggtaaac ccgaaacgcg
     9361 ttttattctt gttgacatgg agctattaaa tcagtcccat tcgccatgcc gaagcatgtt
     9421 gcccagccgg cgccagcgag gaggctggga ccatgccggc caaaagcacc gactcggtgc
     9481 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
     9541 cggagaccac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9601 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     9661 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     9721 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     9781 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     9841 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
     9901 cccggggatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
     9961 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    10021 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    10081 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgggtctcgt cggtgatgtc
    10141 tgctcaagcg gggtagctgt tagtcaagct gcgatgaagt gggaaagctc gaactgaaag
    10201 gttcaaagga ataagggatg ggaaggatgg agtatggatg tagcaaagta cttacttagg
    10261 ggaaataaag gttcttggat gggaagatga atatactgaa gatgggaaaa gaaagagaaa
    10321 agaaaagagc agctggtggg gagagcagga aaatatggca acaaatgttg gactgacgca
    10381 acgaccttgt caaccccgcc gacacaccgg gcggacagac ggggcaaagc tgcctaccag
    10441 ggactgaggg acctcagcag gtcgagtgta gagcaccgga tgggtcgact gccagcttgt
    10501 gttcccggtc tgcgccgctg gccagctcct gagcggcctt tccggtttca tacaccgggc
    10561 aaagcaggag aggcacgata tttggacgcc ctacagatgc cggatgggcc aattagggag
    10621 cttacgccgc tcgctttaag gccgctcccc ccctgaaaga gttgatattg aaggtagtag
    10681 gtacggtgca gggtaacgcg tgatggccgc caccgcggtg gagctccaat tcgccctata
    10741 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    10801 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    10861 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
    10921 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    10981 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    11041 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    11101 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    11161 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    11221 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    11281 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    11341 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
    11401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    11461 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
    11521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
    11581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
    11641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
    11701 aatgatgagc acttttcgac cgaataaata cctgtgacgg aagatcactt cgcagaataa
    11761 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga
    11821 gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
    11881 ggcgtatttt ttgagttgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
    11941 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
    12001 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
    12061 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
    12121 cgcctgatga atgctcatcc ggaattacgt atggcaatga aagacggtga gctggtgata
    12181 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
    12241 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
    12301 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
    12361 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
    12421 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
    12481 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
    12541 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
    12601 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
    12661 aatggcagaa attcgaaagc aaattcgacc cggtcgtcgg ttcagggcag ggtcgttaaa
    12721 tagccgctta tgtctattgc tggtttaccg gtttattgac taccggaagc agtgtgaccg
    12781 tgtgcttctc aaatgcctga ggccagtttg ctcaggctct ccccgtggag gtaataattg
    12841 acgatatgat cctttttttc tgatcaaaaa ggatctaggt gaagatcctt tttgataatc
    12901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    12961 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    13021 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    13081 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    13141 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    13201 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    13261 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    13321 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    13381 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    13441 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    13501 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    13561 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    13621 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    13681 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    13741 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    13801 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    13861 gttagctcac tcattaggca ccccaggctt tacactttat gctcccggct cgtatgttgt
    13921 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    13981 agcgcgcaat taaccctcac taaagggaac aaaagctggg taccgggccc cccctcgagg
    14041 tcgactacat gtatctgcat gttgcatcgg gaaatcccac cacagggaca gccaagcggc
    14101 cccgcgactt ggcagtgggc aaactacgcc cgattctggt gccaagaacc gagaagaatg
    14161 agacagaccc acgttgcact ctaaccggat gctatcgact tacggtggct gaagattcaa
    14221 cacgctgcaa cgagagccaa ggtggtccgg acattttcta cgtgccggtt taccttggaa
    14281 catcgccgtc gttgagtgca cgttgcctac tctctcgtgg cttggctggg cccacgagcc
    14341 cgattgactc gacggtgtta cttgggtatc tatggccccg ttttctggca cggtaatgat
    14401 aagtacttac tagtcttcga gcgggggagt gttgctctgc ccgagcatca acgattggcc
    14461 tgatcgcacc gtctgcaaat gccacggtgc ggaccgactg aaatctcaga ccaccaaaga
    14521 ccctccgact tcgagatacg gttactaatt ttacactggc tccagcggcc ccatccagta
    14581 agcatctggg ctgcaagcgt ataatgtctc caggttgtct cagcataaac accccgcccc
    14641 cgctcaggca cacaggaaga gagctcaggt cgtttccatt gcgtccatac tcttcactca
    14701 ttgtcatctg caggagaact tcccctgtcc ctttgccaag ccctctcttc gtcgttgtcc
    14761 acgccttcaa gttttcacca ttatttttct agacaccatg gccaagttga ccagtgccgt
    14821 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
    14881 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
    14941 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
    15001 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
    15061 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
    15121 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gaccgacgcc
    15181 gaccaacacc gccggtccga cgcggcccga cgggtccgag gcctcggaga tccgtccccc
    15241 ttttcctttg tcgatatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc
    15301 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
    15361 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt
    15421 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
    15481 ggacgctcga aggctttaat ttgcaagctt gatagcttat tttttgtata ctgttttgtg
    15541 atagcacgaa gtttttccac ggtatcttgt taaaaatata tatttgtggc gggcttacct
    15601 acatcaaatt aataagagac taattataaa ctaaacacac aagcaagcta ctttagggta
    15661 aaagtttata aatgcttttg acgtataaac gttgcttgta tttattatta caattaaagg
    15721 tggatagaaa acctagagac tagttagaaa ctaatctcag gtttgcgtta aactaaatca
    15781 gagcccgaga ggttaacaga acctagaagg ggactagata tccgggtagg gaaacaaaaa
    15841 aaaaaaacaa gacagccaca tattagggag actagttaga agctagttcc aggactagga
    15901 aaataaaaga caatgatacc acagtctagt tgacaactag atagattcta gattgaggcc
    15961 aaagtctctg agatccaggt tagttgcaac taatactagt tagtatctag tctcctataa
    16021 ctctgaagct agaataactt actactatta tcctcaccac tgttcagctg cgcaaacgga
    16081 gtgattgcaa ggtgttcaga gactagttat tgactagtca gtgactagca ataactaaca
    16141 aggtattaac ctaccatgtc tgccatcacc ctgcacttcc tcgggctcag cagccttttc
    16201 ctcctcattt tcatgctcat tttccttgtt taagactgtg actagtcaaa gactagtcca
    16261 gaaccacaaa ggagaaatgt cttaccactt tcttcattgc ttgtctcttt tgcattatcc
    16321 atgtctgcaa ctagttagag tctagttagt gactagtccg acgaggactt gcttgtctcc
    16381 ggattgttgg aggaactctc cagggcctca agatccacaa cagagccttc tagaagactg
    16441 gtcaataact agttggtctt tgtctgagtc tgacttacga ggttgcatac tcgctccctt
    16501 tgcctcgtca atcgatgaga aaaagcgcca aaactcgcaa tatggctttg aaccacacgg
    16561 tgctgagact agttagaatc tagtcccaaa ctagcttgga tagcttacct ttgccctttg
    16621 cgttgcgaca ggtcttgcag ggtatggttc ctttctcacc agctgattta gctgccttgc
    16681 taccctcacg gcggatctgc ataaagagtg gctagaggtt ataaattagc actgatccta
    16741 ggtacggggc tgaatgtaac ttgcctttcc tttctcatcg cgcggcaaga caggcttgct
    16801 caaattccta ccagtcacag gggtatgcac ggcgtacgga ccacttgaac tagtcacaga
    16861 ttagttagca actagtctgc attgaatggc tgtacttacg ggccctcgcc attgtcctga
    16921 tcatttccag cttcaccctc gttgctgcaa agtagttagt gactagtcaa ggactagttg
    16981 aaatgggaga agaaactcac gaattctcga cacccttagt attgtggtcc ttggacttgg
    17041 tgctgctata tattagctaa tacactagtt agactcacag aaacttacgc agctcgcttg
    17101 cgcttcttgg taggagtcgg ggttgggaga acagtgcctt caaacaagcc ttcataccat
    17161 gctacttgac tagtcaggga ctagtcacca agtaatctag ataggacttg cctttggcct
    17221 ccatcagttc cttcatagtg ggaggtccat tgtgcaatgt aaactccatg ccgtgggagt
    17281 tcttgtcctt caagtgcttg accaatatgt ttctgttggc agagggaacc tgtcaactag
    17341 ttaataacta gtcagaaact agtatagcag tagactcact gtacgcttga ggcatccctt
    17401 cactcggcag tagacttcat atggatggat atcaggcacg ccattgtcgt cctgtggact
    17461 agtcagtaac taggcttaaa gctagtcggg tcggcttact atcttgaaat ccggcagcgt
    17521 aagctccccg tccttaactg cctcgagata gtgacagtac tctggggact ttcggagatc
    17581 gttatcgcga atgctcggca tactaatcgt tgactagtct tggactagtc ccgagcaaaa
    17641 aggattggag gaggaggagg aaggtgagag tgagacaaag agcgaaataa gagcttcaaa
    17701 ggctatctct aagcagtatg aaggttaagt atctagttct tgactagatt taaaagagat
    17761 ttcgactagt tatgtacctg gagtttggat ataggaatgt gttgtggtaa cgaaatgtaa
    17821 gggggaggaa agaaaaagtc ggtcaagagg taactctaag tcggccattc ctttttggga
    17881 ggcgctaacc ataaacggca tggtcgactt agagttagct cagggaattt agggagttat
    17941 ctgcgaccac cgaggaacgg cggaatgcca aagaatcccg atggagctct agctggcggt
    18001 tgacaacccc accttttggc gtttctgcgg cgttgcaggc gggactggat acttcgtaga
    18061 accagaaagg caaggcagaa cgcgctcagc aagagtgttg gaagtgatag catgatgtgc
    18121 cttgttaact aggtcaaaat ctgcagtatg cttgatgtta tccaaagtgt gagagaggaa
    18181 ggtccaaaca tacacgattg ggagagggcc taggtataag agtttttgag tagaacgcat
    18241 gtgagcccag ccatctcgag gagattaaac acgggccggc atttgatggc tatgttagta
    18301 ccccaatgga aagcctgaga gtccagtggt cgcagataac tccctaaatt ccctgagcta
    18361 actctaagtc gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag
    18421 agttacctct tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt
    18481 cctatatcca aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac
    18541 tagatactta accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg
    18601 tctcactctc accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact
    18661 agtcaacgat tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact
    18721 gtcactatct cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa
    18781 gccgacccga ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc
    18841 ctgatatcca tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga
    18901 gtctactgct atacta
//
