LOCUS       sequence-364389-       11712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myc-tagged BioID2 fused to SARS-CoV-2 NSP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL,
            Halfmann CT, Diaz Pena R, Pratt D, Campos AR, Roux KJ
  TITLE     A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins.
  JOURNAL   Viruses. 2022 Mar 15;14(3). pii: v14030611. doi: 10.3390/v14030611.
  PUBMED    35337019
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses."; 
            date: "2022-03-15"; volume: "14(3). pii: v14030611. doi"; pages: " 
            10.3390/v14030611"
COMMENT     Sequence Label: BioID2-1x-NSP3 pBabe-puro (SARS-CoV-2)
FEATURES             Location/Qualifiers
     source          1..11712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1411..1420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1420..1449
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1453..2148
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     primer_bind     complement(8042..8062)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8047..8376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8227..8362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8386..8985
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8386..8405)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8882..8902
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9206..9675
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9805..9822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9976..10564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10056..10075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10724..11515,11516..11584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11347..11366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11585..11689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acgtaggaat tcggagaccc aagctggcta gccaccatgg aacaaaaact catctcagaa
     1441 gaggatctcg acttcaagaa cctgatctgg ctgaaggagg tggacagcac ccaggagaga
     1501 ctgaaggagt ggaacgtgag ctacggcacc gccctggtgg ccgacagaca gaccaagggc
     1561 agaggcggcc tgggcagaaa gtggctgagc caggagggcg gcctgtactt cagcttcctg
     1621 ctgaacccca aggagttcga gaacctgctg cagctgcccc tggtgctggg cctgagcgtg
     1681 agcgaggccc tggaggagat caccgagatc cccttcagcc tgaagtggcc caacgacgtg
     1741 tacttccagg agaagaaggt gagcggcgtg ctgtgcgagc tgagcaagga caagctgatc
     1801 gtgggcatcg gcatcaacgt gaaccagaga gagatccccg aggagatcaa ggacagagcc
     1861 accaccctgt acgagatcac cggcaaggac tgggacagaa aggaggtgct gctgaaggtg
     1921 ctgaagagaa tcagcgagaa cctgaagaag ttcaaggaga agagcttcaa ggagttcaag
     1981 ggcaagatcg agagcaagat gctgtacctg ggcgaggagg tgaagctgct gggcgagggc
     2041 aagatcaccg gcaagctggt gggcctgagc gagaagggcg gcgccctgat cctgaccgag
     2101 gagggcatca aggagatcct gagcggcgag ttcagcctga gaagaagcct cgagggtgga
     2161 ggcgggtcta ccggtatggc ccctaccaag gtgaccttcg gagacgacac cgtgatcgag
     2221 gtgcagggtt acaagagcgt gaacatcacc ttcgagctgg acgaacgcat cgacaaggtg
     2281 ctgaacgaaa agtgctccgc ttacaccgtg gagctgggca ccgaggtgaa cgaattcgcc
     2341 tgcgtggtgg ccgacgctgt gatcaagacc ctgcagccag tgtccgaact gctgacccct
     2401 ctgggtatcg acctggacga gtggtctatg gctacctact acctgttcga cgagagcggc
     2461 gaattcaagc tggcctctca catgtactgc agcttctacc ctcccgacga agacgaggaa
     2521 gagggtgact gcgaagagga agagttcgag ccttccactc agtacgagta cggcaccgaa
     2581 gacgactacc agggcaagcc tctggaattc ggagccacct ctgccgctct gcagcctgaa
     2641 gaggaacagg aggaagactg gctggacgac gactcccagc agaccgtggg tcagcaggac
     2701 ggctctgaag acaaccagac caccaccatc cagaccatcg tggaggtgca gcctcagctg
     2761 gagatggaac tgaccccagt ggtgcagacc atcgaggtga acagcttctc cggctacctg
     2821 aagctgaccg acaacgtgta catcaagaac gctgacatcg tggaggaagc caagaaggtg
     2881 aaaccaaccg tggtggtgaa cgccgctaac gtgtacctga agcacggcgg aggtgtggct
     2941 ggagctctga acaaggctac caacaacgcc atgcaggtgg aatccgacga ctacatcgct
     3001 accaacggtc ctctgaaggt gggcggatcc tgcgtgctgt ctggccacaa cctggccaag
     3061 cactgcctgc acgtggtggg accaaacgtg aacaagggag aggacatcca gctgctgaag
     3121 agcgcctacg aaaacttcaa ccagcacgag gtgctgctgg ctccactgct gtctgccgga
     3181 atcttcggtg ctgatcccat ccacagcctg cgcgtgtgcg tggacaccgt gaggaccaac
     3241 gtgtacctgg ccgtgttcga caagaacctg tacgacaagc tggtgagctc cttcctggag
     3301 atgaagtccg aaaagcaggt ggagcagaag atcgccgaga tccccaagga ggaagtgaag
     3361 cctttcatca ccgaatctaa gcccagcgtg gagcagagaa agcaggacga caagaagatc
     3421 aaggcctgcg tggaggaagt gaccaccacc ctggaggaaa ccaagttcct gaccgagaac
     3481 ctgctgctgt acatcgacat caacggcaac ctgcaccctg actccgctac cctggtgtct
     3541 gacatcgaca tcaccttcct gaagaaggac gccccctaca tcgtgggcga cgtggtgcag
     3601 gaaggagtgc tgaccgctgt ggtcatccct accaagaagg ccggtggcac caccgagatg
     3661 ctggccaagg ctctgcgtaa ggtgccaacc gacaactaca tcaccaccta ccccggtcag
     3721 ggcctgaacg gttacaccgt ggaggaagct aagaccgtgc tgaagaagtg caagtctgcc
     3781 ttctacatcc tgccctccat catctctaac gagaagcagg aaatcctggg caccgtgagc
     3841 tggaacctgc gcgaaatgct ggcccacgct gaggaaacca ggaagctgat gcccgtgtgc
     3901 gtggagacca aggccatcgt gagcaccatc cagagaaagt acaagggcat caagatccag
     3961 gagggtgtgg tggactacgg cgctcgtttc tacttctaca cctccaagac caccgtggcc
     4021 tctctgatca acaccctgaa cgacctgaac gagaccctgg tgaccatgcc actgggatac
     4081 gtgacccacg gtctgaacct ggaggaagcc gctagataca tgcgttccct gaaggtgcct
     4141 gctaccgtga gcgtgagcag ccctgacgct gtgaccgcct acaacggata cctgacctcc
     4201 tctagcaaga cccctgagga acacttcatc gaaaccatca gcctggccgg ttcctacaag
     4261 gactggtctt acagcggaca gtccacccag ctgggcatcg agttcctgaa gcgcggagac
     4321 aagagcgtgt actacacctc caacccaacc accttccacc tggacggtga agtgatcacc
     4381 ttcgacaacc tgaagaccct gctgtctctg cgcgaggtga ggaccatcaa ggtgttcacc
     4441 accgtggaca acatcaacct gcacacccag gtggtggaca tgagcatgac ctacggacag
     4501 cagttcggtc ctacctacct ggacggagct gacgtgacca agatcaagcc acacaactcc
     4561 cacgagggca agaccttcta cgtgctgcct aacgatgaca ccctgagagt ggaggctttc
     4621 gaatactacc acaccaccga cccatccttc ctgggcaggt acatgtctgc cctgaaccac
     4681 accaagaagt ggaagtaccc ccaggtgaac ggactgacca gcatcaagtg ggccgacaac
     4741 aactgctacc tggccaccgc tctgctgacc ctgcagcaga tcgaactgaa gttcaaccca
     4801 cccgctctgc aggacgctta ctacagagcc cgtgctggcg aggccgctaa cttctgcgct
     4861 ctgatcctgg cctactgcaa caagaccgtg ggcgaactgg gagacgtgag agagaccatg
     4921 agctacctgt tccagcacgc caacctggac tcctgcaagc gtgtgctgaa cgtggtgtgc
     4981 aagacctgcg gccagcagca gaccaccctg aagggagtgg aagctgtgat gtacatgggt
     5041 accctgtcct acgagcagtt caagaagggc gtgcagatcc cttgcacctg cggaaagcag
     5101 gccaccaagt acctggtgca gcaggaaagc ccattcgtga tgatgtccgc tcctccagcc
     5161 cagtacgaac tgaagcacgg caccttcacc tgcgcttccg agtacaccgg caactaccag
     5221 tgcggacact acaagcacat cacctctaag gagaccctgt actgcatcga cggagccctg
     5281 ctgaccaagt cctctgaata caagggtccc atcaccgacg tgttctacaa ggagaacagc
     5341 tacaccacca ccatcaagcc cgtgacctac aagctggacg gcgtggtgtg caccgaaatc
     5401 gaccctaagc tggacaacta ctacaagaag gacaactctt acttcaccga gcagcctatc
     5461 gacctggtgc caaaccagcc ctaccctaac gccagcttcg acaacttcaa gttcgtgtgc
     5521 gacaacatca agttcgctga cgacctgaac cagctgaccg gatacaagaa gcctgcctct
     5581 agagagctga aggtgacctt cttcccagac ctgaacggcg acgtggtggc tatcgactac
     5641 aagcactaca ccccaagctt caagaaggga gccaagctgc tgcacaagcc catcgtgtgg
     5701 cacgtgaaca acgctaccaa caaggccacc tacaagccta acacctggtg catccgttgc
     5761 ctgtggtcta ccaagccagt ggaaacctcc aactctttcg acgtgctgaa gagcgaggac
     5821 gctcagggca tggacaacct ggcctgcgaa gacctgaagc ccgtgagcga ggaagtggtg
     5881 gagaacccaa ccatccagaa ggacgtgctg gaatgcaacg tgaagaccac cgaggtggtg
     5941 ggagacatca tcctgaagcc agctaacaac agcctgaaga tcaccgagga agtgggtcac
     6001 accgacctga tggccgctta cgtggacaac agctccctga ccatcaagaa gcccaacgag
     6061 ctgagcaggg tgctgggtct gaagaccctg gctactcacg gtctggctgc tgtgaactct
     6121 gtgccatggg acaccatcgc taactacgcc aagcctttcc tgaacaaggt ggtgtccacc
     6181 accaccaaca tcgtgaccag atgtctgaac cgcgtgtgca ccaactacat gccctacttc
     6241 ttcaccctgc tgctgcagct gtgcaccttc accagaagca ccaactcccg tatcaaggct
     6301 agcatgccta ccaccatcgc caagaacacc gtgaagagcg tgggaaagtt ctgcctggag
     6361 gcttccttca actacctgaa gtctccaaac ttcagcaagc tgatcaacat catcatctgg
     6421 ttcctgctgc tctccgtgtg cctgggttcc ctgatctact ctaccgccgc tctgggagtg
     6481 ctgatgtcta acctgggtat gcctagctac tgcactggtt acagagaggg atacctgaac
     6541 agcaccaacg tgaccatcgc cacctactgc accggcagca tcccatgctc cgtgtgcctg
     6601 tctggactgg acagcctgga cacctacccc tccctggaaa ccatccagat caccatctct
     6661 agcttcaagt gggacctgac cgccttcggt ctggtggctg agtggttcct ggcctacatc
     6721 ctgttcacca ggttcttcta cgtgctgggc ctggccgcta tcatgcagct gttcttctcc
     6781 tacttcgctg tgcacttcat cagcaactcc tggctgatgt ggctgatcat caacctggtg
     6841 cagatggctc ccatctctgc catggtgcgc atgtacatct tcttcgccag cttctactac
     6901 gtgtggaagt cctacgtgca cgtggtggac ggttgcaact cctctacctg catgatgtgc
     6961 tacaagcgca acagggctac cagggtggag tgcaccacca tcgtgaacgg cgtgcgcagg
     7021 tccttctacg tgtacgccaa cggcggcaag ggattctgca agctgcacaa ctggaactgc
     7081 gtgaactgcg acaccttctg cgctggctcc accttcatct ctgacgaggt ggccagagac
     7141 ctgagcctgc agttcaagcg tccaatcaac cccaccgacc agagctccta catcgtggac
     7201 tctgtgaccg tgaagaacgg tagcatccac ctgtacttcg acaaggctgg ccagaagacc
     7261 tacgagcgcc actctctgag ccacttcgtg aacctggaca acctgagggc caacaacacc
     7321 aagggcagcc tgcctatcaa cgtgatcgtg ttcgacggaa agtccaagtg cgaggaatct
     7381 agcgccaagt ccgccagcgt gtactacagc cagctgatgt gccagccaat cctgctgctg
     7441 gaccaggctc tggtgtctga cgtgggagac agcgccgaag tggccgtgaa gatgttcgac
     7501 gcctacgtga acaccttctc ctctaccttc aacgtgccta tggagaagct gaagaccctg
     7561 gtggctactg ctgaggctga actggctaag aacgtgtccc tggacaacgt gctgagcacc
     7621 ttcatctccg ccgctaggca gggtttcgtg gactctgacg tggaaaccaa agacgtggtg
     7681 gagtgcctga agctgtctca ccagagcgac atcgaagtga ccggcgactc ctgcaacaac
     7741 tacatgctga cctacaacaa ggtggagaac atgaccccaa gagacctggg agcttgcatc
     7801 gactgctccg cccgtcacat caacgctcag gtggccaagt ctcacaacat cgccctgatc
     7861 tggaacgtga aggacttcat gagcctgtcc gagcagctgc gcaagcagat caggagcgcc
     7921 gctaagaaga acaacctgcc cttcaagctg acctgcgcta ccaccagaca ggtggtgaac
     7981 gtggtgacca ccaagatcgc cctgaagggc ggataagttt aaaccgctga gtcgaccctg
     8041 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8101 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8161 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8221 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8281 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8341 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag
     8401 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     8461 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     8521 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     8581 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     8641 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     8701 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     8761 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     8821 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     8881 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     8941 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     9001 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgataaaata aaagatttta
     9061 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     9121 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
     9181 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     9241 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     9301 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     9361 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     9421 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     9481 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     9541 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     9601 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     9661 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat
     9721 ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9781 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    10441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc
    10681 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    11281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    11341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    11461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    11521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    11581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11701 gaaaagtgcc ac
//
