LOCUS       sequence-36439-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F3-GCG
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(297..317)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(297..315)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(336..352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(336..352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(349..371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    360..376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(384..390,391..408,409..414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    429..450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(567..584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttgccctgt cgagtcctgc gatcgccgct tttctcgctc ggatgagctt acccgccata
       61 tccgcatcca taccggtcag aagcccttcc agtgtcgaat ctgtatgcgt aacttcagtc
      121 gtagtgacca ccttaccacc cacatccgca cccacacagg cgagaagcct tttgcatgcg
      181 acatctgcgg caggaagttc gcgcgctctg acgacctcac ccgtcacacc aagatccaca
      241 cgggtgagaa ataatctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
      301 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      661 gataacgcag gaaagaacat gtgagcaaag ggccagcaaa aggccaggaa ccgtaaaaag
      721 gccgcgttgc tgg
//
