LOCUS       sequence-364397-       10270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3264
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      41..176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             222..824
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(222..241)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     718..738
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    867..984
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1122..2300
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1353..2291
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2248..2268
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2320..2344
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2345..2354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4145..4169)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4177..4218
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4329..4917
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4382..4402)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4800..4811)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4989..5222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5300..5421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5337..5356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5391..5410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5461..5596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5616..5635)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5617..5635)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5645..5662)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5645..5661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5654..5676)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5803..6258
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5890..5909)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6100..6121
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6284..6388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6389..6457,6458..7249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6607..6626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7420..8008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7909..7928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8162..8179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8296..8317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8332..8337,8338..8355,8356..8362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8370..8386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8375..8397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8394..8410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8394..8410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8429..8449
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8431..8449
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8477..8703
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8704..8884
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8931..9056
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9549..9782
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9967..10011
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(10242..10270,1..182)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
ORIGIN
        1 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
       61 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      121 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      181 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
      241 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
      301 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
      361 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
      421 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
      481 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
      541 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
      601 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
      661 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
      721 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
      781 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
      841 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
      901 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      961 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     1021 cgccggctcg aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt
     1081 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     1141 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     1201 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     1261 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     1321 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     1381 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     1441 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     1501 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     1561 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     1621 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     1681 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     1741 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     1801 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     1861 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     1921 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     1981 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     2041 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     2101 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     2161 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     2221 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     2281 ttccatttca ggtgtcgtga ggctagcatc gattgatcaa caagtttgta caaaaaagtt
     2341 ggcaccatgg cggcggaaga ggaggctgcg gcgggaggtg aggttgccgg tggcgaggcg
     2401 acggccccag gtaaagtgtt gagagaggag aaccagtgca ttgctcctgt ggtttccagc
     2461 cgcgtgagtc cagggacaag accaacagct atggggtctt tcagctcaca catgacagag
     2521 tttccacgaa aacgcaaagg aagtgattca gacccatccc aagaagctca tagccaaact
     2581 gaaaagcgga ggagagataa aatgaataac ctgattgaag aactgtctgc aatgatccct
     2641 cagtgcaacc ccatggcgcg taaactggac aaacttacag ttttaagaat ggctgttcaa
     2701 cacttgagat ctttaaaagg cttgacaaat tcttatgtgg gaagtaatta tagaccatca
     2761 tttcttcagg ataatgagct cagacattta atccttaaga ctgcagaagg cttcttattt
     2821 gtggttggat gtgaaagagg aaaaattctc ttcgtttcta agtcagtctc caaaatactt
     2881 aattatgatc aggctagttt gactggacaa agcttatttg acttcttaca tccaaaagat
     2941 gttgccaaag taaaggaaca actttcttct tttgatattt caccaagaga aaagctaata
     3001 gatgccaaaa ctggtttgca agttcacagt aatctccacg ctggaaggac acgtgtgtat
     3061 tctggctcaa gacgatcttt tttctgtcgg ataaagagtt gtaaaatctc tgtcaaagaa
     3121 gagcatggat gcttacccaa ctcaaagaag aaagagcaca gaaaattcta tactatccat
     3181 tgcactggtt acttgagaag ctggcctcca aatattgttg gaatggaaga agaaaggaac
     3241 agtaagaaag acaacagtaa ttttacctgc cttgtggcca ttggaagatt acagccatat
     3301 attgttccac agaacagtgg agagattaat gtgaaaccaa ctgaatttat aacccggttt
     3361 gcagtgaatg gaaaatttgt ctatgtagat caaagggcaa cagcgatttt aggatatctg
     3421 cctcaggaac ttttgggaac ttcttgttat gaatattttc atcaagatga ccacaataat
     3481 ttgactgaca agcacaaagc agttctacag agtaaggaga aaatacttac agattcctac
     3541 aaattcagag caaaagatgg ctcttttgta actttaaaaa gccaatggtt tagtttcaca
     3601 aatccttgga caaaagaact ggaatatatt gtatctgtca acactttagt tttgggacat
     3661 agtgagcctg gagaagcatc atttttacct tgtagctctc aatcatcaga agaatcctct
     3721 agacagtcct gtatgagtgt acctggaatg tctactggaa cagtacttgg tgctggtagt
     3781 attggaacag atattgcaaa tgaaattctg gatttacaga ggttacagtc ttcttcatac
     3841 cttgatgatt cgagtccaac aggtttaatg aaagatactc atactgtaaa ctgcaggagt
     3901 atgtcaaata aggagttgtt tccaccaagt ccttctgaaa tgggggagct agaggctacc
     3961 aggcaaaacc agagtactgt tgctgtccac agccatgagc cactcctcag tgatggtgca
     4021 cagttggatt tcgatgccct atgtgacaat gatgacacag ccatggctgc atttatgaat
     4081 tacttagaag cagagggggg cctgggagac cctggggact tcagtgacat ccagtggacc
     4141 ctcttgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     4201 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     4261 tggctttata tatcttgtgg aaaggacgaa cacacgatgt acagatacct tatacgcgtt
     4321 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4381 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4441 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4501 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4561 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4621 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4681 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4741 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4801 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4861 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     4921 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4981 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     5041 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5101 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5161 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5221 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     5281 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     5341 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5401 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5461 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5521 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5581 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5641 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5701 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5761 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     5821 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5881 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5941 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6001 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6061 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6121 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6181 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6241 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6361 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6901 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7561 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8041 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8161 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8221 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8281 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8341 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8401 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8461 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     8521 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     8581 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     8641 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     8701 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8761 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8821 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8881 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8941 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     9001 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     9061 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     9121 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9181 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9241 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     9301 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     9361 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     9421 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     9481 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     9541 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     9601 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     9661 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     9721 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     9781 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     9841 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     9901 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     9961 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    10021 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    10081 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    10141 tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca
    10201 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10261 gcatgcgtct
//
