LOCUS       sequence-36441-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     pcDNA-HA-Ubc7
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HA-UBE2L3
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             111..137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(636..654)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(637..654)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..691)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    680..906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcgagctctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tagcttggta ccacgcgtac catggatatc tatccatatg
      121 acgttccaga ttatgctgct cagatcgata agcttatggc ggccagcagg aggctgatga
      181 aggagcttga agaaatccgc aaatgtggga tgaaaaactt ccgtaacatc caggttgatg
      241 aagctaattt attgacttgg caagggctta ttgttcctga caacccccca tatgataagg
      301 gagccttcag aatcgaaatc aactttccag cagagtaccc attcaaacca ccgaagatca
      361 catttaaaac aaagatctat cacccaaaca tcgacgaaaa ggggcaggtc tgtctgccag
      421 taattagtgc cgaaaactgg aagccagcaa ccaaaaccga ccaagtaatc cagtccctca
      481 tagcactggt gaatgacccc cagcctgagc acccgcttcg ggctgaccta gctgaagaat
      541 actctaagga ccgtaaaaaa ttctgtaaga atgctgaaga gtttacaaag aaatatgggg
      601 aaaagcgacc tgtggactaa tctagagggc cctattctat agtgtcacct aaatgctaga
      661 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      721 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      781 gaaattgcat cgcattgtct gagtaggtgt cattctattc tgggggggtg gggtggggca
      841 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggaat gcggtgggct
      901 ctatggcttc tgaggcggaa
//
