LOCUS       sequence-364420-        1968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus protein expression of mouse TLR4 (residues 26–544) fused
            with hagfish variable lymphocyte receptor (VLR) (residues 126–200).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Wang Y, Su L, Morin MD, Jones BT, Whitby LR, Surakattula MM, Huang 
            H, Shi H, Choi JH, Wang KW, Moresco EM, Berger M, Zhan X, Zhang H, 
            Boger DL, Beutler B
  TITLE     TLR4/MD-2 activation by a synthetic agonist with no similarity to 
            LPS.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E884-93. doi: 
            10.1073/pnas.1525639113. Epub 2016 Feb 1.
  PUBMED    26831104
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1525639113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "E884-93"
COMMENT     Sequence Label: pAcGP67A-mTLR4ecd-VLR
FEATURES             Location/Qualifiers
     source          1..1968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     55..114
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             1927..1947
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1948..1965
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgctactag taaatcagtc acaccaaggc ttcaataagg aacacacaag caagatggta
       61 agcgctattg ttttatatgt gcttttggcg gcggcggcgc attctgcctt tgcggcggat
      121 cccaatccct gcatagaggt agttcctaat attacctacc aatgcatgga tcagaaactc
      181 agcaaagtcc ctgatgacat tccttcttca accaagaaca tagatctgag cttcaacccc
      241 ttgaagatct taaaaagcta tagcttctcc aatttttcag aacttcagtg gctggattta
      301 tccaggtgtg aaattgaaac aattgaagac aaggcatggc atggcttaca ccacctctca
      361 aacttgatac tgacaggaaa ccctatccag agtttttccc caggaagttt ctctggacta
      421 acaagtttag agaatctggt ggctgtggag acaaaattgg cctctctaga aagcttccct
      481 attggacagc ttataacctt aaagaaactc aatgtggctc acaattttat acattcctgt
      541 aagttacctg catatttttc caatctgacg aacctagtac atgtggatct ttcttataac
      601 tatattcaaa ctattactgt caacgactta cagtttctac gtgaaaatcc acaagtcaat
      661 ctctctttag acatgtcttt gaacccaatt gacttcattc aagaccaagc ctttcaggga
      721 attaagctcc atgaactgac tctaagaggt aattttaata gctcaaatat aatgaaaact
      781 tgccttcaaa acctggctgg tttacacgtc catcggttga tcttgggaga atttaaagat
      841 gaaaggaatc tggaaatttt tgaaccctct atcatggaag gactatgtga tgtgaccatt
      901 gatgagttca ggttaacata tacaaatgat ttttcagatg atattgttaa gttccattgc
      961 ttggcgaatg tttctgcaat gtctctggca ggtgtatcta taaaatatct agaagatgtt
     1021 cctaaacatt tcaaatggca atccttatca atcattagat gtcaacttaa gcagtttcca
     1081 actctggatc taccctttct taaaagtttg actttaacta tgaacaaagg gtctatcagt
     1141 tttaaaaaag tggccctacc aagtctcagc tatctagatc ttagtagaaa tgcactgagc
     1201 tttagtggtt gctgttctta ttctgatttg ggaacaaaca gcctgagaca cttagacctc
     1261 agcttcaatg gtgccatcat tatgagtgcc aatttcatgg gtctagaaga gctgcagcac
     1321 ctggattttc agcactctac tttaaaaagg gtcacagaat tctcagcgtt cttatccctt
     1381 gaaaagctac tttaccttga catctcttat actaacacca aaattgactt cgatggtata
     1441 tttcttggct tgaccagtct caacacatta aaaatggctg gcaattcttt caaagacaac
     1501 accctttcaa atgtctttgc aaacacaaca aacttgacat tcctggatct ttctaaatgt
     1561 caattggaac aaatatcttg gggggtattt gacaccctcc atagacttca attattaaat
     1621 atgagtcaca acaatctatt gtttttggat tcatcccatt ataaccagct gtattccctc
     1681 gatcccggga aagaactggc gctggatacc aaccagctga aaagcgtgcc ggatggcatt
     1741 tttgatcgcc tgaccagcct gcagaaaatt tggctgcata ccaacccgtg ggattgcagc
     1801 tgcccgcgca ttgattatct gagccgctgg ctgaacaaaa acagccagaa agaacagggc
     1861 agcgcgaaat gcagcggcag cggcaaaccg gtgcgcagca ttatttgccc gaccgaattc
     1921 gcgggcgaaa acttgtactt tcaaggccat caccatcacc atcactag
//
