LOCUS       sequence-364422-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  baculovirus protein expression of mouse MD-2 (residues 19–160) fused
            to a protein A tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Wang Y, Su L, Morin MD, Jones BT, Whitby LR, Surakattula MM, Huang 
            H, Shi H, Choi JH, Wang KW, Moresco EM, Berger M, Zhan X, Zhang H, 
            Boger DL, Beutler B
  TITLE     TLR4/MD-2 activation by a synthetic agonist with no similarity to 
            LPS.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E884-93. doi: 
            10.1073/pnas.1525639113. Epub 2016 Feb 1.
  PUBMED    26831104
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1525639113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "E884-93"
COMMENT     Sequence Label: pAcGP67A-mMD2-proA
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             433..453
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             487..660
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             661..834
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
ORIGIN
        1 gagaagcaac agtggttctg caactcctcc gatgcaatta tttcctacag ttattgtgat
       61 cacttgaaat tccctatttc aattagttct gaaccctgca taagactgag gggaaccaat
      121 ggatttgtgc atgttgagtt cattccaaga ggaaacttaa aatatttata tttcaaccta
      181 ttcatcagtg tcaactccat agagttgccg aagcgtaagg aagttctgtg ccatggacat
      241 gatgatgact attctttttg cagagctctg aaaggagaga ctgtgaatac atcaatacca
      301 ttctctttcg agggaatact atttcctaag ggccattaca gatgtgttgc agaagctatt
      361 gctggggata ctgaagaaaa gctcttctgt ttgaatttca ccatcattca ccgccgtgat
      421 gtcaataagg gcgaaaactt gtactttcaa ggcgaattcg caggccttgc gcaacacgat
      481 gaagccgtgg acaacaaatt caacaaagaa caacaaaacg cgttctatga gatcttacat
      541 ttacctaact taaacgaaga acaacgaaac gccttcatcc aaagtttaaa agatgaccca
      601 agccaaagcg ctaacctttt agcagaagct aaaaagctaa atgatgctca ggcgccgaaa
      661 gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt acatttacct
      721 aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga cccaagccaa
      781 agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac
      841 gcgtga
//
