LOCUS       sequence-364439-        9396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9396)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX_403
FEATURES             Location/Qualifiers
     source          1..9396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    71..89
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    107..125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    178..196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    214..232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    249..267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    285..303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    365..482
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(695..739)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    924..1157
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1650..1775
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1822..2002
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2003..2229)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2257..2277)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2257..2275)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2296..2312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2296..2312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2309..2331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2320..2336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2344..2350,2351..2368,2369..2374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2389..2410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2527..2544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2698..3286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2778..2797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3457..4248,4249..4317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4080..4099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4318..4389)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4389..4524
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4564..4685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4575..4594)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4629..4648
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4757..4990
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5195..5206
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5604..5624
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5664..5672,5673..5708,5709..5711,5712..5747,
                     5748..5750,5751..5786,5787..5792,5793..6410))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6411..6464)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6465..7061)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6545..6565)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7042..7061
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7071..7581)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7134..7152)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7591..7608)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7622..7663)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    7668..7792
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7833..8138)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7935..7954)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(8480..9139)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9054..9073
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(9193..9199,9200..9217,9218..9223))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(9248..9372)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(join(9393..9396,1..311))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(join(9396,1..24))
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct
       61 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
      121 agggataaac tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
      181 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
      241 gacatcgttc tctatcactg atagggagta aactcgacat acgttctcta tcactgatag
      301 ggagtaaact cgacatatcg attcgcggcc aaagtggatc tctgctgtcc ctgtaataaa
      361 cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg
      421 ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa
      481 aattgtggat gaatactgcc atttgtctcg aggtcgagaa ttgtcccctc ggggttggga
      541 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
      601 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
      661 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
      721 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
      781 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct
      841 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
      901 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
      961 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
     1021 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
     1081 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
     1141 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
     1201 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
     1261 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt
     1321 gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     1381 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     1441 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     1501 tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat
     1561 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     1621 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     1681 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     1741 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     1801 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     1861 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     1921 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     1981 tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc
     2041 tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta
     2101 ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc
     2161 cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat
     2221 aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg
     2281 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2341 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2401 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2461 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3121 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4381 atttgaaagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     4441 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     4501 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggatagcta gagccagaca
     4561 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4621 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4681 aagttcctct cactctctga tattcatttc tttgcaagtt ataaatactg aataataaga
     4741 tgacatgaac tactactgct agagattttc cacactgact aaaagggtct gagggatctc
     4801 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     4861 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     4921 tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg ggagtgaatt
     4981 agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa
     5041 gtcattggtc ttaaaggtac ctctagtccg gacgccgagg cgaaacaggc ggggaggcgg
     5101 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag
     5161 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga
     5221 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca
     5281 gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc
     5341 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc
     5401 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca
     5461 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca
     5521 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca
     5581 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaatacgat
     5641 atcttggatc gatccttact tagttacccg gggagcatgt caaggtcaaa atcgtcaaga
     5701 gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct
     5761 aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc
     5821 aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg
     5881 aacagctcaa ttgcttgtct cagaagtggg ggcatagaat cggtggtagg tgtctctctt
     5941 tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc
     6001 acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg
     6061 agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta
     6121 gccccgtcgc gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag
     6181 tcttgccatg actcgccttc cagggggcag aagtgggtat gatgcctgtc cagcatctcg
     6241 attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc
     6301 tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gacaccattg
     6361 agtaattcca gagctccgtt tatgactttg ctcttgtcca gtctagacat tggaccaggg
     6421 ttttcttcaa catcaccaca agtgaggaga gaacctctac cttcggcacc gggcttgcgg
     6481 gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg
     6541 agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg
     6601 gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg
     6661 tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc
     6721 gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg
     6781 cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc
     6841 gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg
     6901 aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg
     6961 cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg
     7021 tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tggtgaattg ctggggagag
     7081 aggtcggtga ttcggtcaac gagggagccg actgccgacg tgcgctccgg aggcttgcag
     7141 aatgcggaac accgcgcggg caggaacagg gcccacacta ccgccccaca ccccgcctcc
     7201 cgcaccgccc cttcccggcc gctgctctcg gcgcgccctg ctgagcagcc gctattggcc
     7261 acagcccatc gcggtcggcg cgctgccatt gctccctggc gctgtccgtc tgcgagggta
     7321 ctagtgagac gtgcggcttc cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct
     7381 tcccgactta ggggcggagc aggaagcgtc gccggggggc ccacaagggt agcggcgaag
     7441 atccgggtga cgctgcgaac ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg
     7501 cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg gctgccttgg
     7561 aaaaggcgca accccaaccc cggatcctta gtggtggtgg tggtggtgga ccggtctatg
     7621 ctgtactgtc gagacctagg agtgggtttg ggattggctt tcctgcaacc actttgtaca
     7681 agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     7741 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct
     7801 gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg
     7861 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag
     7921 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
     7981 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
     8041 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
     8101 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
     8161 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
     8221 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
     8281 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
     8341 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag
     8401 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta
     8461 gtaagccgga tccagatctt tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     8521 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     8581 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     8641 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     8701 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     8761 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     8821 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     8881 tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca
     8941 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     9001 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     9061 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     9121 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgac ggatcctaac
     9181 tcaaaatcca cacattatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9241 cggccgccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     9301 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttttt
     9361 gtacaaactt gttgatgcta gccaattctc caggcg
//
