LOCUS       sequence-364445-        6295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For production of AAV for a fluorescent sensor for oxytocin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Ino D, Tanaka Y, Hibino H, Nishiyama M
  TITLE     A fluorescent sensor for real-time measurement of extracellular 
            oxytocin dynamics in the brain.
  JOURNAL   Nat Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 
            10.1038/s41592-022-01597-x.
  PUBMED    36138174
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 
            10.1038/s41592-022-01597-x."
COMMENT     Sequence Label: pAAV.Syn.MTRIA-OT
FEATURES             Location/Qualifiers
     source          1..6295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     582..598
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     583..599
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1552..1573
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1679..1700)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2457..2473)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2457..2473)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    2475..3063
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2528..2548)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2946..2957)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3287..3306)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3558..3698
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3558..3687
                     /label=AAV2 ITR
     rep_origin      3773..4228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3860..3879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4070..4091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4245..4264)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4364..4386
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4424..4442)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4510..4614
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4615..4683,4684..5475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4833..4852)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5646..6234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6135..6154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcaa ttgggtacca ggatgaggcg gggtgggggt gcctacctga
      181 cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc
      241 tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag
      301 caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg
      361 aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc caccttggtc
      421 gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc
      481 acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg
      541 gcagcggagg agtcgtgtcg tgcctgagag cgcagctgtg ctcgaggtcg acggtatcga
      601 taagcttgat atcgaattca ccatggagat catttccaat gaaagtgaga tctggcagtt
      661 caacggatcg tggaggaact caagcctcgg taacggcacc ggcgctctga atcagacgaa
      721 ccccctgaag cggaacgagg aggtggcgaa ggtggaggtg acggtgctgg cgctggtgct
      781 gtttctggcg ctggcgggga acctgtgcgt cctgctggcc atccacacca ccaaacacag
      841 ccagtcccgc atgtattact tcatgaagca cctgagcatc gccgatctag ttgtggcaat
      901 atttcaagtt ctcccccaac ttatctggga catcacattc cggttttatg gacctgacat
      961 tttgtgcagg ttggtgaaat acctgcaggt ggttggaatg ttcgcctcca cgtacatgtt
     1021 agttttaatg tccatcgaca gatgtttggc catctgtcag cctctgcact ccttgcatcg
     1081 gagaaaagat cgaatttatg tcatattgtc atggttgctg agtttgatct tcagcatccc
     1141 gcagatgttc atcttctccc tcagagaggt tggctcggcc gggtcaggag tgtacgactg
     1201 ctggggcgac tttgtgaagc cctggggggc caaagcttac atcacatgga tcagtctcac
     1261 catctacatc atcccagtgg ccatcctgag catttgttac gggctgatct gctacaaaat
     1321 atggcgaaat ttcaaaggga aattaaacgt ctatatcaag gccgacaagc agaagaacgg
     1381 catcaaggcg aacttccaca tccgccacaa catcgaggac ggcggcgtgc agctcgccta
     1441 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1501 cctgagcgtg cagaccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct
     1561 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg
     1621 taccggaggg atgatggtga gaaagggcga ggagctgttc accggggtgg tgcccatcct
     1681 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg
     1741 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1801 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1861 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga
     1921 gcgcaccatc ttcttcaagg acgacggcca ctacaagacc cgcgccgagg tgaagttcga
     1981 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2041 catcctgggg cacaagctgg agtacaactc agtaatctcc aaggcaaaga tacgcacagt
     2101 gaaaatgact tttgtgatag tcgtggcata catcgtctgc tggaccccgt ttttctctgt
     2161 ccagatgtgg tctgcctggg atcaagcggc tccccgtgag gccatgccct tcatcatctc
     2221 catgctgctg gcgagcctga acagctgctg caacccctgg atctacatgt gctttgccgg
     2281 ccacctcttc catgaccttc ggcagaacct cctgtgctgc tccactcgct acctcaagtc
     2341 acccgagtgc cgctgcgagc gagatttcaa ttctagtcac aaaagcaact cctcaaactt
     2401 cgccattaag agcacaagca gcagcagaag cattactcag acttccacca catgaggatc
     2461 cactagttct agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2521 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2581 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2641 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2701 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2761 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2821 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     2881 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2941 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3001 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3061 cgctagcttt ctcgctagca gatctacggg tggcatccct gtgacccctc cccagtgcct
     3121 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3181 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3241 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3301 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3361 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3421 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3481 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3541 accactgctg cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3601 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3661 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     3721 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     3781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3841 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3901 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3961 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     4021 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4081 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     4141 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4201 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     4261 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4321 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4381 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     4441 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4501 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4561 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4621 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4681 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4741 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4801 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4861 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4921 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4981 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5041 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5101 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5161 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5221 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5281 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5341 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5401 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5461 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5521 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5581 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5641 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5701 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5761 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5821 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5881 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5941 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6001 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6061 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
