LOCUS       sequence-36445-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   McLaughlin KJ, Strain-Damerell CM, Xie K, Brekasis D, Soares AS, 
            Paget MS, Kielkopf CL
  TITLE     Structural basis for NADH/NAD+ redox sensing by a Rex family 
            repressor.
  JOURNAL   Mol Cell. 2010 May 28. 38(4):563-75.
  PUBMED    20513431
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 May 28. 38(4):563-75."
COMMENT     Sequence Label: pSKB2-TRexR90D
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(735..758)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(768..785)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             804..826
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    846..862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(866..885)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(867..885)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atnctctttg ggcttgtgca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcc tatcacccca tcatctcttc ccgccacttg
      121 gggttcagga tagcgaaaga aagccgcgtg agccccgcca agaagtccac gttctccacc
      181 gccacctcct tgggcacctc gaggaccacc ggggcgaagt tcaggatccc cttgatcccc
      241 gccgccacca acaggtccgc cgccttctgg gccgcttccc ggggcacggt gaggagggcg
      301 atctcaatgc ggccgggaac ccgctggggc aggaggtcca cgtgctcaat gaccccgccc
      361 cgcaccgggc ggcccacctt ctccgggtcc acgtcaaaga agcccctaag ctcaaagctc
      421 tccccgaacc ccgggtagtc ggccagggcg ctcccgaggt cgcccatgcc cacgatgcac
      481 aggccccact tgcggttgag ccccaggatg tggcggagct cccgcttcag gacggggacg
      541 gtgtacccca ccccgcgcgt cccgtaggag ccgaagtagg aaaggtcctt ccgcacctgg
      601 aaggccgtga cctgggcgag ctccccaagc tgctcggagc tcgtccggtg aaccccctgg
      661 gcctccagct cctccaagat gcggaggtag gtgatcaggc ggctgatggc cgcttcgggg
      721 accttcatca tatggggccc ctggaacaga acttccaggc cgctgctgtg atgatgatga
      781 tgatggctgc tgcccatggt atatctcctt cttaaagtta aacaaaaatt atttctagag
      841 ggnaattgtt atccgctcac aattccccta tagtgagtcg tattaanttt ncgcgggatc
      901 gagatntcga tcctntacgc cggangcatc gtggccgnna cgccagccca aatgg
//
