LOCUS       sequence-364486-       11897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of dSarm(isoE,K450R)::FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-dsarm(E,K450R)::flag
FEATURES             Location/Qualifiers
     source          1..11897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4892..4986
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5005..5243
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5190..5211
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5657..5677
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             8419..8442
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          8733..8798
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8928..8948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9220..9354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9257..9276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9311..9330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9421..9490
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9709..9727)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9748..9764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9748..9764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9761..9783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9772..9788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9796..9802,9803..9820,9821..9826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9841..9862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9979..9996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10150..10738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10230..10249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10909..11700,11701..11769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11532..11551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11770..11874)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaaaagc
     4861 tttccggatc caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
     4921 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     4981 tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt ctacggagcg
     5041 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
     5101 aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta
     5161 aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta
     5221 ttgaatacaa gaagagaact ctgaataggg aattgggaat tcatgtccaa tcaggcaccc
     5281 tggcccgttc gcaagggcat cttccgctcg agcggtcagt cggatttcac gccaacccga
     5341 tcgcctagtc ccattgtgga gatgccatta tccccgccgc cggtggccac gccctcgaat
     5401 agatttggaa tcaattcacc gctctcccca ccgccgcagc ctattcaagt ggtggcgggt
     5461 tcaacgacag ccactaccat gtccactgcg tccgctgcca gagtgtctgg tgcagctgcc
     5521 tccacttcat cctcgtcctc ctcttcttcg tcgtcgcagt gctcgtcgca aacctcgtcg
     5581 agttctagtt cgcacaccag agtacgtaaa agttctaacc cgcccccaca gccaattacc
     5641 agttgtccac tgtcgccacc accaccacca ccaccacagc aacaacaaca actaccacaa
     5701 caactgccac ctccaacgcc catcaacaac accaaccact cagcaattac aacacccaat
     5761 cacaacaata accacaactg caatcaaatg agaaatgtac gcgaaattcc cattgaggtg
     5821 gagcagagca aggaagccct ctcactccgc accggcgaca tcacacagca ggcgagcaac
     5881 aatgtggcgg cctccagtat tacggtgcaa agtgagaatt tctccgcgga caagaaggcg
     5941 atatcccagt cgcagcaatc gcagacgatg acctccaacg ggatcatcag tcaggagaaa
     6001 catgtatcct ccgcctcgca ggccaactac tcgatgtcgc acaagggcgt ttccagtacc
     6061 ggcagtagca tgatcaccag ctcctcgcaa atgtccgcga tgaatggcca gatgctgaag
     6121 ctcgccgatc tcaagttgga tgacctcaag tcgctgacag ctggcagtgg gcagcaggag
     6181 atcgagcaga ccatcaacaa gtattccaat atgctgacct caatagtgag ctccctgcaa
     6241 gaagatgagc gaggtggtag tgccataacc gtccacgatg ttggtggcaa gaagtctcag
     6301 tacctggaga agatcaacga agtcattcgt cgagcttggg cagtgcccac acatggccac
     6361 gagctgggct actcattgtg caattccctg cgccaaagtg gcggcctcga tcttctcatg
     6421 aagaactgcg ttaagcccga tctccagttt tcctccgcac agcttcttga gcagtgccta
     6481 accaccgaga accgcaaaca tgtggtggat aatggtctgg ataaggtggt caatgtggcc
     6541 tgtgtctgca caaagaattc caatatggag cactcccgcg tgggcactgg catcctggag
     6601 catctgttca gacactccga gggcacctgt tcggatgtga taaggctagg tggtctggat
     6661 gccgtgctct ttgagtgccg caccagtgat ttggaaactc tgcgacactg cgctagtgca
     6721 ctggcaaatt tatctctata tggtggagcc gagaatcagg aggagatgat cctacgcaag
     6781 gtgcccatgt ggttgttccc attggccttc cacaacgatg ataacatcaa gtactatgcc
     6841 tgcttggcca ttgcagtgct ggtagccaac aaggagattg aagccgaggt gctgaagtct
     6901 ggatgcttgg atttggtgga accctttgtc acctcccacg acccctcggc ttttgcgaga
     6961 tccaatctgg cacatgccca cgggcaaagc aaacattggc tgaaaagatt ggttccagtt
     7021 ttgagttcca atcgcgaaga ggcccgcaat ctggctgcct tccatttttg catggaggcg
     7081 ggcatcaaga gggagcaggg taacaccgac atcttccgcg agatcaacgc tattgaagct
     7141 ttgaaaaatg tggccagctg tccaaatgcc attgcatcca agttcgccgc tcaggctctg
     7201 agattgattg gagagacagt tccgcacaag ctgtcccaac aggttccctt gtggtccgtg
     7261 gaggatgtgc aggagtgggt aaagcaaatc ggtttcaatg actacatcga caagttcaac
     7321 gagtcccagg tggacgggga tcttttactg aagctcaatc aggataacct ccgggctgat
     7381 attggaatcg gtaatggaat acttctaaag cgttttgaac gcgagctgca aaatctcaag
     7441 cgtatggctg actactcatc caaggacacg gccaagatgc accaatttct ctcggagatc
     7501 ggtactgatt actgcaccta cacgtatgcc atgctgaatg ctggaattga caagtgtgca
     7561 ctgccgcatg tcaatgagga tatgctgatg acagagtgtg gcatccacaa ctcgatccat
     7621 cgtctgagaa ttctcaatgc ggtcaaaaac ttggagaatt cgctgcccag ctcgtcggag
     7681 gagaacatgg ccaagacgct agatgttttc gttagctata ggcgatcaaa cggctcccaa
     7741 ctagccagtc ttctcaaggt gcatctgcag cttcgtggtt tctccgtttt catcgacgtg
     7801 gagcgcctgg aggcgggcaa atttgacaac ggcctattga acagtattcg acaggcaaag
     7861 aactttgtct tagtattaac tcccgatgcc ctgcaccgct gcattaacga cgaggactgc
     7921 aaggactggg tgcatcgcga aatcgtggct gccctgaact cgaactgcaa cattataccc
     7981 atcatagacc agcaattcga ttggcccgag gtggagcgac ttcccgagga catgcgcagt
     8041 gtggcccatt ttaacggtgt taattggatc cacgactacc aggacgcatg catcgataag
     8101 ctcgaaagat ttttgcgcgg cgaaaagaat atcgatcgca ttgcggcgat ggtgcccggc
     8161 acccccggtt cggtctcata ccaaagaatg cacagcaacg actccgacta ccagagcgga
     8221 ggagcaggcg ccggatcagg agcaggaact ggaggaggag gtggtggcgg cgttacgggc
     8281 agtgtggtgg atggcctgat ggtggcggcc aatggcagcg gacaaggtgg gccaacgagt
     8341 actacatcta ctacttcttc tactcccaac tcaaattcaa gctcaagctc taaccagagt
     8401 cccccagcgg ctccagcgga ctacaaagac gatgacgaca agtaggaatt ccggcgatgc
     8461 tgatccgagc agtccgatgg ggaggaggag gaggaggagg aggacggcga tgaggaggag
     8521 gaagatgatg gatccgatga ggaaaatgga aatggaaata gagggggcag aaatcgaccc
     8581 agagacaacg gaggatgaga tgatgatccc acatagactt gttgcattaa acgaatatta
     8641 gcgcttatgt ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     8701 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     8761 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     8821 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     8881 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     8941 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     9001 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     9061 tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata
     9121 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     9181 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     9241 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9301 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcggatcc
     9361 gcggccgcgg ctcgagggta cctctagaga tccactagtg tcgacgatgt aggtcacggt
     9421 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
     9481 ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa
     9541 cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag
     9601 cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc
     9661 gacggtcacg gcgggcatgt cgacactagt tctagccagc ttttgttccc tttagtgagg
     9721 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9781 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10561 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10801 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10861 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11881 tccccgaaaa gtgccac
//
