LOCUS       sequence-364495-       10442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Fanning AS, Ma TY, Anderson JM
  TITLE     Isolation and functional characterization of the actin binding 
            region in the tight junction protein ZO-1.
  JOURNAL   FASEB J. 2002 Nov . 16(13):1835-7.
  PUBMED    12354695
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2002 Nov . 16(13):1835-7."
COMMENT     Sequence Label: pEGFP ZO1
FEATURES             Location/Qualifiers
     source          1..10442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        131..322
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     279..299
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(324..428)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      455..910
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(542..561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     752..773
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    917..1038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(928..947)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     982..1001
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     RBS             5209..5217
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(join(6939..7649,7650..7652,7653..7655))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6939..7649,7650..7652,7653..7655))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6981..7002)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7330..7349
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7589..7610
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7652..7661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7700..7903)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7729..7749)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7904..8207
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(8382..8970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8462..8481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    9299..9346
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     9371..9390
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(9578..10372)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9578..10372)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9689..9708)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10299..10318
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(10407..10442,1..322))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10421..10442,1..114)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
       61 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
      121 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
      181 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
      241 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
      301 cagctggttc tttccgcctc aggactcttc ctttttcaat attattgaag catttatcag
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      421 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
      481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      601 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
      781 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
      841 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
      901 cttacaattt acgcgttaag atacattgat gagtttggac aaaccacaac tagaatgcag
      961 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     1021 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     1081 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     1141 gatcagttat ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cataataaaa
     1201 taaaatgtgt acgtatgcta ggccagggcc atagtaaagt ttgaaacagt gtactttgga
     1261 aagaacagac ctcaaatgca cccccattta ctggctggta ttttaacgga aatcaatatg
     1321 tgaagttaag cagtgacgat aaaaaaatta caaaaatcac aaagcaaaat atctttgaac
     1381 ctctagccaa taccaacagt cccgtcaatc acaaacatgc agtgtgtagc atgttttccg
     1441 accatggttc aggggcatgc tcactcatct ttatcagttc aaacaaatgc ctcatactaa
     1501 caaactgtag tatcaactct aaaaaaggta taatacttga tagagtggtt ccatttagat
     1561 taagtttaat ccagtttcac attatttaag ttcctatatt tcaagagtta aaagtggtca
     1621 ataaggacag aaacacagtt tgctccaacg agataatttg gatctccggg aagacacttg
     1681 ttttgccagg ttttaggatc acccgacgag gagtcggatg attttagagc aaaagaccaa
     1741 ccgtcaggag tcatggacgc acagtgtggt aagcgcagct ccacaggctt caggaacttg
     1801 aggccatggg gaccacacat caccaaagga ctcagcagtg tttcaccttt ctctttatct
     1861 aaaggtggaa ggatgctgtt gtcccggcag accttgaaat agatttcctg ctcaactcct
     1921 tcgggaatgg ctccttgagg gataattata ctaacaccag tttctatgga actcagcacg
     1981 cccccattgc tgttaaatat gcctcgggct gtggccacca cagtatgacc atcttcatct
     2041 tcatcctctt ccacagctga aggactcaca ggaatagctt taggcactgt gctgatatta
     2101 tttatttgat atttaggctt ctctgcatgg atagagaaag tttcaactcc actgtcaaac
     2161 tcaggaggct gtgccaaact gtgcgatttc acaagagttt ttggagaagt gggagttttt
     2221 gaagacaagt caggtttatg tgcagtttca cttggcagaa gattgtgatt gaatttagga
     2281 ctttcaaact tgcgttcaaa tggtcgggca gaacttgtat atggttttgg tgtgaatcga
     2341 ttgtatgctg gagtgactgt tttctgagtc tgttcagttc cattaactgg ggctttatct
     2401 ggaaaacttt tctggggata gaaagctgcc tgagcagtat cttctcggtt tggtggtctg
     2461 aaagttgctg gcttattctg agatggaggt gggtctggtt tggacactaa ggaattctga
     2521 aaatccaatg acactgaatt accttcacca tgtgctccct tagaatgtat gtggagagac
     2581 gcgctggtga caggctgaga tggctgggca tactgcgagg gcaatggagg aggaggggga
     2641 gtggcctgga tgggttcata gcgtttctcg ccaaatgatc tatccacacc atcagcttca
     2701 ggaggctttc cctttgaaga ataactagaa aaatttgatt gattctgaga atgagctggc
     2761 tttgcaggct cggagagatg gctggcagca atgtgtgcag gaggcttatt ctcaaaactt
     2821 cttcggtcaa agtatgacag ctgttttcga taatattctt catcttcttc agggtcataa
     2881 tgattggacc gaacaatatc ttcaggtggc ttcagttgag gtttttgagg ttctgggatc
     2941 ctgtacagag ttttgtcatg ttcactgaat tgattctgag aagtgggttt gggaccaatg
     3001 atgggagcac ctgaaggttt agatgctact tctggaggct taaaactgcc agtgtcattt
     3061 acatccttct tggtctctaa ggatgcagat cttttgtttt caaacatctt aactctggtg
     3121 agtacagact gtggcttcat tgctggatct tcctcttctt cggtttgagt tgggggtgga
     3181 ggcagtggtt tggtgtttga aggcagagct tctggcttat gctgagatga aggtatcagc
     3241 ggagggacag ctgcagcacc atggagaggc tcaaaatgac ctgctctaga ggtaaatcct
     3301 tggggtggta cttgctcgta actgcgtgaa tattgctcaa aatactgctt ggactctgca
     3361 ggcttgggcc ctgctgaagg gtggggctgg gcttccggtc tgagtctacc atgtgtgtca
     3421 tacccaggag ctggctgctc ttcgtgccgc agggcggatg ctctaggtgc ctgttcgtaa
     3481 cgtggtctgc tgtcgtaaga cagaggggct ggctcttcaa aacgtggaaa gtaccctcgt
     3541 tctgaggact cttcgggatg ctgtctggag tcaaggtctt gagagtgctg attatcaaaa
     3601 ggtggccgag atgggtaggg ctgtttgtca tcataatatg accactgttc ttcatacatg
     3661 gggacgcgat cttcgtatcg cagacgatgt tcatagtttc gagaaaactg gtccgtatag
     3721 cttgaggact cgtatctgta tgtgggctgc tcgaggtctc tgctggcttg tttctctacg
     3781 tatgggagtt ggggttcata ggtcagatta ggctctttgt ctggcctgtg ccctgggtga
     3841 ctaacggctg gctgtttcaa aacatggttc tgcctcatca tttcctcggg atatggatcc
     3901 tttctataca cctttgttgg atctacatgc gacgacaatg atggttcttg atctcttagc
     3961 attatgtgag ctgcctcagt acttggtgtt cttaaagaat cagcttgtgg tgagtaagag
     4021 gtggaaggag ctggggtggg ctcctccagt ctgacattag ttaaatttac attatgatta
     4081 acagcagagg ttgatgatgc tgggtttgtt tcaggcgaaa ggtaagggac tggagatgaa
     4141 gcttctgctt tctgttgaga ggctggctta aatccagggg agtctattct atgaattggt
     4201 tgtggctgcg cttgtggtga gtaaggagga tatgtttggt tttcatgatg cattccagag
     4261 gagtcctctc ttacaggctc agaggaccgt gtaatggcag actccggtgg agtcccaacc
     4321 tcatcattaa gagtttcatc tagttcttga tcagtgtagg ccccgccttc tgtgtctgtg
     4381 tcttcatagt cagaagtgtg tctactgtcc gtgctataca ttgagtattc actacctgga
     4441 gctgacaggt aggacagacg atcatcatgc aaatcaaggt catcacttgt agcaccatcc
     4501 gcctttccct cggaaaccca taccagctgg ttttgctgtt gttgaattgc ttctttcagc
     4561 gcaccatacc aaccatcatt cattgaattt aagttaattg tagttgtaaa aagatggtga
     4621 ttatttttac gaagtttatg agatcgctcg tataacttcc tggcactttt ccgagattct
     4681 ggacataacc tcattctcat tgtttttact ccttgcttag aatcagggtt aagaaataca
     4741 acaattggat accactgggc atagttaaga cgatcaactg catttggtgt tacatctaat
     4801 aaagcatgtt tgtcttgatc tatgatttgc tttattgtat gcaggcgaat aatgccagag
     4861 ctacgttggt cagttccagc gtctcgtggt tcactctttg caatttgata aatatctggt
     4921 tcttctcttg ccagcttttc tctggcaaca tcagctattg gtccaaaaat ggttacaggc
     4981 ctcagaaatc cagcttctcg aagaaccact ctttcataag ctggaaactt tgtttgaaca
     5041 ggctgagcgg acaaatcctc tctgcttttt cgaagatttc tcttggagct gcgaagacct
     5101 ctgaatctcc agaagtcagc acggtctccg cctgctgttt ttggaagtgt atactgtaca
     5161 ctggctagct gctcagctct gttcttatta gggatgatgc ctcgttctac ctccttatga
     5221 tttttaccaa ttcgaatagc aagccaagag cccagttttc cattgtacaa ggtatccaca
     5281 acacggaaca cctctccttt gttaaaacta agtccatagg gagattcctt ttcatattca
     5341 aaatgggttc taatatagaa agaatctcct acatctgatt ctacaatgcg acgataaaca
     5401 tccttcttct tctgagccaa tatggtcact tcttctcctt tagggaggtc aagcaggaaa
     5461 aggacggctt cttctcttat gatatttgta aaatctacgt tgtttaccct gagaatttga
     5521 tcaccttcct ctaagccttc cttggctgca gggctatctt ctagaacgcc agctacaaat
     5581 attccaacat catttccacc agccagccgc aaacccacac tatctccttt tctgaatttt
     5641 accaatttca tgctgggccg aagaatccca tcttcatgag ttgaattagg taggacacca
     5701 tcagatggac tgacaggtaa atccacatct ggttgcccaa cttgggcata cacaggcttt
     5761 ggttctggaa gagaaggtgt ttgtttctca tttctttcaa ctgtaacttc ttccactgtt
     5821 ttaggtgtgt gatcatcagc atgctttaca ggagttgaga cagccccagg tttagaaatt
     5881 ctctcttcat ctctactccg gagactgcca ttgcttggct gctgcggcga gtgcctggaa
     5941 tgatcagaag gctctgaccg ctggtcagga gatcgtgacc ggctgcggcg gggaggccta
     6001 tcgtgtgatc gaccagaatg atctgatgcc agtgactgaa tttctgaaat gtcgtctctc
     6061 tcagaggcat tagcagagtg gatgctgtca gaaagatcag ggacattcaa tagcgtagcc
     6121 cgttcatctc tttgaactac catttttaat ttgcctttag acctttctat caatgtcttt
     6181 gcatctgtca atgacatatt ttctgtcaca gtaccattta tcttcaatac aacatcacct
     6241 tcttgaatat tgccatctct tgctgccaaa ctatcttgtg aaatttcctt aacaaatata
     6301 tggcttgcca atcgaagacc atattcttca tttttccggg atttcaccag tgtgacttta
     6361 gtaggtttag caggctggct ggaagccact gaccgcctgt ctgaccgcgg ggacaagctc
     6421 ctctctctac ttgcacttct atccctcggc caaatcttct cactccttct gttaaccaca
     6481 ccactccggc cacttcttgg atcatgtatt tcctcatcat aactatcttc ttcattatca
     6541 gatactggtt caggatcagg acgacttact ggtatttgaa ctttcttctt ccttctaatt
     6601 gtaatttttg catttttccc acttttcctt agttgctgaa cagcaaaagc atgttcaaca
     6661 ttatccattg aaactccgtt aaccattgca actcggtcat tttcctgtag ctgtccttca
     6721 gctggtcctc ctttcagcac atctgaaatc actattgacg tttccccact ctgaaaatga
     6781 ggattatctc gtccaccaga tattgcaatt ccaaatccaa atccaggagc cctgtgaagc
     6841 gtcactgtat gttgttccca tatagctgtt tcctccattg ctgtgctctt ggcggccgca
     6901 gctctggcgg acatctgagc tcgagatctg agtccggact tgtacagctc gtccatgccg
     6961 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     7021 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
     7081 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
     7141 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
     7201 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     7261 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     7321 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     7381 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
     7441 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
     7501 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     7561 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     7621 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtag cgctagcgga
     7681 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     7741 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     7801 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     7861 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     7921 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     7981 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     8041 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     8101 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     8161 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     8221 atgggctatg aactaatgac cccgtaattg attactatta ataactaatg catggcggta
     8281 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9121 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct
     9181 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa
     9241 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
     9301 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
     9361 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac
     9421 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     9481 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     9541 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     9601 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     9661 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     9721 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     9781 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     9841 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     9901 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     9961 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
    10021 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
    10081 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
    10141 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
    10201 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
    10261 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
    10321 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
    10381 atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct
    10441 ca
//
