LOCUS       sequence-364496-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Direct conversion of fibroblasts to functional neurons by defined 
            factors.
  JOURNAL   Nature. 2010 Feb 25. 463(7284):1035-41.
  PUBMED    20107439
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Feb 25. 463(7284):1035-41."
COMMENT     Sequence Label: Tet-O-FUW-Ascl1
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    298..415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(713..757)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    942..1175
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1668..1793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1840..2020
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2038..2236)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2238..2617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2789..2808
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2883..2987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2988..3056,3057..3848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3206..3225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4019..4607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4508..4527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4761..4778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4895..4916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4931..4936,4937..4954,4955..4961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4969..4985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4974..4996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4993..5009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4993..5009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5058..5179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5069..5088)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5123..5142
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5309..5683)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(5702..5749)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5797..6126)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5811..5946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5865..5884)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6111..6131
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6140..6568)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6250..6271)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6462..6481
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6614..6838
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6827..6844
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             6870..7050
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     7555..7571
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7556..7572
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7575..8163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7681..7692
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8090..8110
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      8880..8889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8972..8996)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8993..9031)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(9064..9082,9083..9093,1..12,13..31,32..54,55..73,
                     74..96,97..115,116..138,139..157,158..180,181..199,200..222,
                     223..241)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
       61 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
      121 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
      181 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
      241 agtggtaaac tcgagatcta accaaactgg atctctgctg tccctgtaat aaacccgaaa
      301 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
      361 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
      421 gatgaatact gccatttgtc tgcagaattg gcgcacgcag tgccgatccg ttcactaatc
      481 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
      541 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
      601 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
      661 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
      721 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
      781 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
      841 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
      901 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
      961 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     1021 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     1081 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     1141 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     1201 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     1261 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     1321 tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     1381 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     1441 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     1501 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     1561 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     1621 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     1681 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     1741 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     1801 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     1861 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     1921 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     1981 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg
     2041 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     2101 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     2161 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     2221 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     2281 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     2341 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     2401 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     2461 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     2521 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     2581 attgattact attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca
     2641 tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag
     2701 ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa
     2761 gcagggagca gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     2821 cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
     2881 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2941 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3001 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3061 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3121 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3181 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3241 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3301 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3361 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3421 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3481 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3541 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3601 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3661 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3721 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3781 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3841 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3901 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4141 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4681 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4741 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4801 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4861 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     4921 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4981 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg
     5041 acggtataca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5101 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     5161 aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga
     5221 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     5281 cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc
     5341 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
     5401 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
     5461 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
     5521 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
     5581 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
     5641 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag ttcctcacct
     5701 tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgatc
     5761 agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta ggcctccaaa
     5821 aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg cctctgcata
     5881 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     5941 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     6001 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     6061 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     6121 acacacattc cacagaatta attcgcgtta aatttttgtt aaatcagctc attttttaac
     6181 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6241 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6301 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6361 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6421 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6481 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6541 gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt
     6601 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     6661 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     6721 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     6781 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     6841 gaggctgatc agcgggttta aacgggccct gctagagatt ttccacactg actaaaaggg
     6901 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     6961 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     7021 ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct ctcgggccat
     7081 gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct ccgggtcatc
     7141 catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat tggcttcttc
     7201 taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     7261 gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg tgtggtagat
     7321 ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca gtcccccctt
     7381 ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc ttaaaggtac
     7441 ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt gcttctagcc aggcacaatc
     7501 agcattggta gctgctgtat tgctacttgt gattgctcca tgtttttcta ggtctcgagg
     7561 tcgacggtat cgatgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     7621 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     7681 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     7741 ggcgagcagc caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc
     7801 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     7861 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     7921 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     7981 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     8041 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     8101 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     8161 attatcgata agcttgatat cgaattctca gaaccagttg gtaaagtcca gcagctcttg
     8221 ttcctctggg ctaagagggt cgtaggatcc ctcgtcggag gagtaggacg agaccggaga
     8281 acccgccata gagttcaagt cgttggagta gttgggggag atggtgggcg acaggacgcc
     8341 cgcctgaaag gcagcgctca ccgcgtcgtg ctcgtccagc agctgctgca gcgcgcggat
     8401 gtactcgacc gccgagcgca gcgtctccac cttgctcatc ttcttgttgg ccgcgccgtt
     8461 ggggacatgc tcccggaggg tggcaaagcc caggttgacc aacttgaccc ggttgcgctc
     8521 gcgctcgttg cggcgcgcca cggcggccgg ctgctgctgt ggcaggctgt agccgaagcc
     8581 gctgaagttg agccggcgtt tgcagcgcat cagttccgga gaggacgagc gctggcgctt
     8641 gacctgcttg gccgctgact tgtgaccgcc ccctgagggc tggctgtcgg ccaccgggct
     8701 cagctgcggc gcctgctgcg gcggcgcctg cggctgttgc tgctgcgcgc tctgagctgc
     8761 cgcggccgcc gccgctgccg ccgccgccgc ggtcgcaaag aagcaggctg cgggaggcag
     8821 gaagggctgc gggggctgcg gctgctggcc ggctccactc tccatcttgc cagagctctc
     8881 catggtggct agcgaattcg gggccgcgga ggctggatcg gtcccggtgt cttctatgga
     8941 ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc
     9001 ttatataggc ctcccaccgt acacgcctac ctcgacccgg gtaccgagct cgactttcac
     9061 ttttctctat cactgatagg gagtggtaaa ctc
//
