LOCUS       sequence-364513-        6600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Berkovich E, Monnat RJ Jr, Kastan MB
  TITLE     Roles of ATM and NBS1 in chromatin structure modulation and DNA 
            double-strand break repair.
  JOURNAL   Nat Cell Biol. 2007 Jun;9(6):683-90. Epub 2007 May 7.
  PUBMED    17486112
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2007-06"; volume: "9(6)"; pages: "683-90. Epub 2007 
            May 7"
COMMENT     Sequence Label: pBABe-HA-ER-IPpoI
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     191..211
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(1729..1755)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1783..2199)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1799..1815)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1831..1853)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2400..2599
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             2664..3133
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        3164..3268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3269..3337,3338..4129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3487..3506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4289..4877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4778..4797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5031..5048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             5178..5647
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(5868..6467)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5951..5971)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6448..6467
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(6477..6600,1..206))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(6491..6600,1..26)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
       61 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      121 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      181 gactttccac accctaactg acacacattc cacagggtcg accactgtgc tggcgaattc
      241 ctacgtacca ccacactggg atctttatac cacaaagtga ctgccccttt gttgaggacc
      301 tgccacagtc gcccccgggc cgtacaacgg accctgcctt aaacaaacca ccgcatggac
      361 acatcccccg ttgggacccg ggcaccagtt tctgcctttg ttgtcgtcta gtgactccca
      421 gcacaagtgc aagggattgt ggcatcgagt attatgacat aggtgcgatg ctgtgcaggt
      481 tttgccgttg atgttatccg gttctaatag aaaagggact gtgtgggatc cccatctgtg
      541 gacgacttga ttgattgtcc gtttgtattg ccagcgggta ggcccattct tagcaaagcc
      601 aaccccataa ggagctgcta gggggatctc gtaacaatgg agcgttcctt gcagaccgcc
      661 acctaatggt acatggtgcg ttatcactgg aaactgaccg actgtttctt cccagctgtc
      721 aatcacagcc aagatttgag cattggtgag cgccatagat ccgatcgtgt tggggaagcc
      781 ctctgcttcc gggggtatgt agtaggtttg taaggaatgt gctgaagtgg agctggtggt
      841 ggccagctgg gtctggctgg gctcctctgg gggcactccc atgcgactgg ctggggcatg
      901 aaggcggtgg gcatccaaca tctccaggag caggtcatag aggggtacca cgttcttgca
      961 tttcatgttg tagagatgct ccatgcgttt gttactcata tgccggatat gggaaagaat
     1021 gagaaggagc tgagctaggc ggcgatgctg ctgctgcaga gtcaggccag ctttggccat
     1081 caggtggatc aaagtgtctg tgatcttgtc caggacacgg tggatgtggt ccttctcttc
     1141 cagagacttc aaggtgctgg acagaaacgt gtacactccg gaattaagca aaatgatgga
     1201 tttgaggcac acaaactctt caccctgcag gttcatcatg cggaaccgac ttgacgtagc
     1261 aagcaacatg tcaaagatct ccaccatgcc ttccacacat ttaccttgat tcctgtccag
     1321 gagcaagtta ggagcaaaca ggagcttccc cgggtgttcc atggagcgcc agacgagacc
     1381 aatcatcaga atctccagcc aggcacactc gagaaggtgg acctgatcat ggagattcaa
     1441 gtccccaaag cctggcactc tctttgccca gttgatcata tgaaccagct ccctatctgc
     1501 taggttggtc aataagccca tcattgaggc ttcactgaag ggtctagaag gatcatattc
     1561 agaatagatc atgggcggtt cagcatccaa caaggcactg accatctggt cagctgtcaa
     1621 ggacaaggca gggctattct tcttagtgtg cttaatcaca agagggcttg gccaaaggtt
     1681 ggcagccctc atgtctcctg aagcacccat ttcatttcga gatccgctag cgtaatctgg
     1741 aacatcgtat gggtatgcca tggtgaattc gatgatccgg ccggcgccta gagaaggagt
     1801 gagggctgga taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga
     1861 cggggcggat ggaggaagag gaggcggagg cttagggtgt acaaagggct tgacccaggg
     1921 aggggggtca aaagccaagg cttcccaggt cacgatgtag gggacctggt ctgggtgtcc
     1981 atgcgggcca ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc ggttaaaggt
     2041 gccgtctcgc ggccatccga cgttaaaggt tggccattct gcagagcaga aggtaaccca
     2101 acgtctcttc ttgacatcta ccgactggtt gtgagcgatc cgctcgacat ctttccagtg
     2161 acctaaggtc aaacttaagg gagtggtaac agtctggccc taattttcag acaaatacag
     2221 aaacacagtc agacagagac aacacagaac gatgctgcag cgctgcagca gacaagacgc
     2281 gcggcttcgg ttccaaaccg aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt
     2341 ctcgtctcct accagaacca catatcctga cggggtcgga ttccacatcg actcccttcc
     2401 tcaggtcggg ccacaaaaac ggcccccaaa gtccctggga cgtctcccag ggttgcggcc
     2461 gggtgttcag aactcgtcag ttccaccacg ggtccgccag atacagagct agttagctaa
     2521 ctagtaccga cgcaggcgca taaaatcagt catagacact agacaatcgg acagacacag
     2581 ataagttgct ggccagcttg cctcccggtg gtgggtcggt ggtccctggg caggggtctc
     2641 ccgatcccgg acgagccccc aaatgaaaga cccccgctga cgggtagtca atcactcaga
     2701 ggagaccctc ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt
     2761 ggatacacgg gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt
     2821 tgtgggctct tttattgagc tcggggagca gaagcgcgcg aacagaagcg agaagcgaac
     2881 tgattggtta gttcaaataa ggcacagggt catttcaggt ccttggggca ccctggaaac
     2941 atctgatggt tctctagaaa ctgctgaggg ctggaccgca tctggggacc atctgttctt
     3001 ggccctgagc cggggcagga actgcttacc acagatatcc tgtttggccc atattcagct
     3061 gttccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
     3121 ggcccatatt caggctgcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3181 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3241 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3301 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3361 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3421 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3481 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3541 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3601 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3661 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3721 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3781 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3841 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3901 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3961 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4021 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4081 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4141 agtttactca tatatacttt agattgattt gcggccgcaa aaggatctag gtgaagatcc
     4201 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4261 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4321 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4381 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4441 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4501 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4561 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4621 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4681 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4741 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4801 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4861 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4921 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4981 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5041 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5101 ttcattaatg caactatggc catttaatgt aaatacttaa gaaaaaaaac caaattaatt
     5161 ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact cagaggagac
     5221 cctcccaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac
     5281 acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag gggttgtggg
     5341 ctcttttatt gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg
     5401 gttagttcaa ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga
     5461 tggttctcta gaaactgctg agggctggac cgcatctggg gaccatctgt tcttggccct
     5521 gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc agctgttcca
     5581 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
     5641 tattcagctg ttccatctgt tcctgacctt gatctgaact tctctattct cagttatgta
     5701 tttttccatg ccttgcaaaa tggcgttact taagctagct tgccaaacct acaggtgggg
     5761 tctttcattc cccccttttt ctggagacta aataaaatct tttattttat cgatgcatgg
     5821 ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc
     5881 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     5941 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     6001 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     6061 ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg
     6121 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     6181 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     6241 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     6301 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg
     6361 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg
     6421 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagctttttg
     6481 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     6541 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
//
