LOCUS       sequence-364530-       11732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human JMJD3 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Agger K, Cloos PA, Christensen J, Pasini D, Rose S, Rappsilber J, 
            Issaeva I, Canaani E, Salcini AE, Helin K
  TITLE     UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene 
            regulation and development.
  JOURNAL   Nature. 2007 Oct 11. 449(7163):731-4.
  PUBMED    17713478
REFERENCE   2  (bases 1 to 11732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Oct 11. 449(7163):731-4."
COMMENT     Sequence Label: pCMV-HA-JMJD3
FEATURES             Location/Qualifiers
     source          1..11732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(731..760)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(734..763)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(737..766)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(740..769)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(743..772)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1121..1132
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1568..1592)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1605..1631)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          1711..2283
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2374..2398)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2395..2598)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2424..2444)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2599..2978
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    3271..3318
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3343..3362
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3556..4350)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3667..3686)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4277..4296
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4407..4552)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     primer_bind     complement(4963..4980)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4963..4979)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4972..4994)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5188..5207)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5307..5329
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5367..5385)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5453..5557
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5558..5626,5627..6418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5776..5795)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6589..7177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7078..7097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7331..7348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7465..7486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7501..7506,7507..7524,7525..7531)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7539..7555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7544..7566
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7563..7579
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7563..7579
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7919..7938
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7937..7992
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8038..8057
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     protein_bind    8156..8180
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(10968..10997)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(10971..11000)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ccaaagaaga gctcttctaa gatctctcca tcctctcggg ctgcccggca ggccggaccc
       61 ttcaaccagg cagggggtgg tggggggcgt aagaggcggg gacagggggg tggagggggt
      121 gaggaaggtc caggtggcag ggataggtgg ggagtggcag cgggagcccc caagaacggt
      181 ttccgactgc tgtgtgccga gggccccagg cggccatggt gagagacctt cagcgcggga
      241 gtttggtaat ggtcagcgcc gggaatgcct gggttcggct ccacgccccg gagaccagtg
      301 ttgctgctgc tgctactgct gctgctggtg gtggtgccgg ggaggccagg gggccgggaa
      361 ggggcgtaag gcacgcaggc ggtggttgct gctggtgaaa cgctggagtc catccgcgac
      421 ctctgaactc tgctctctct aaggtcactt ccggggggac gggtggcagg caggcagcca
      481 tggctctctg ctcctggggg gcgggggcct gggcctgggg gagcagccgg gaccagccgg
      541 tggccagggg ggtgcgcggt gtacgctgga gctgggtatg gatatgggtg aggcagcgag
      601 tgccgctgct cctggcgctc tgggggtgct gaacccttgc gctctgggcc ccaggcatct
      661 ccatgcaggg tactccacag ccctggcttg gttagctgaa atggggggct ggtggccagg
      721 ccaggcaggg gtggtggtgg tggtggtggt ggtggtggtg gtggtggtgg tggtaatggt
      781 ggtggaggca gtggcagccc tgggggaagg ccagtctgtt cagagttgca gcctctcctt
      841 cgcttgcctc cagggctgag gccctcctcc cctgaggggc ctgagagtgc tgcaggaggc
      901 acaggctgca ccactggggg ttcagcagct cgcttcaccg ggggacctcc ccgcttggct
      961 ccatagttcc gtttgtgctc aaggtgtagc aagttccaca cttgctccag tgggggcagg
     1021 accttggctc ggtgctggca ggagccagta tgaaagttcc agagctgggc ctgctgcagt
     1081 cggccaatgc ggggccccag ctcagcgaag cttcctccgt atcgaagggc gctgtggtag
     1141 cagcgtgtgg cctcctcact atcgtgctct gactcgtaca gttgcccaag ctgttcccaa
     1201 agccctggct gggctggctc ccggagcaat gcctgcacac agccatgtag ggattccagc
     1261 ttcccatgga ggggtcttgg agtgggcgcc cctggagcat aatatggttt gctggggtgc
     1321 ccagaactac tgccatgtga agggggtagg ggagcaggaa gcgggggctg cccaatgctg
     1381 gctgagcatc tgcctccagg cagccatgcg ctacgaggag ggggatgagg cgggcaggag
     1441 ctccaggccc cagcacagct caggccccca agggcaaagg cttcccgtgc agcgcgggcc
     1501 cctggaggat ccactgcccg atgcatccag gtaccggatc cagtcgactg aattggttcc
     1561 tttaaagagc ctgctttttt gtacaaactt gttgatgatc cgctagcgta atctggaaca
     1621 tcgtatgggt atgccatggt gaattgatcc gtcgaggaat tctttgccaa aatgatgaga
     1681 cagcacaaca accagcacgt tgcccaggag ctgtaggaaa gagaagaagg catgaacatg
     1741 gttagcagag ggacccggtt tggactcaga gtattttatc ctcatctcaa acagtgtata
     1801 tcattgtaac cataaagaga aaggcaggat gatgaccagg gtgtagttgt ttctaccaat
     1861 aagaatattt ccacgccagc cagaatttat atgcagaaat attctacctt atcatttaat
     1921 tataacaatt gttctctaaa actgtgctga agtacaatat aatataccct gattgccttg
     1981 aaaaaaaagt gattagagaa agtacttaca atctgacaaa taaacaaaag tgaatttaaa
     2041 aattcgttac aaatgcaagc taaagtttaa cgaaaaagtt acagaaaatg aaaagaaaat
     2101 aagaggagac aatggttgtc aacagagtag aaagtgaaag aaacaaaatt atcatgaggg
     2161 tccatggtga tacaagggac atcttcccat tctaaacaac accctgaaaa ctttgccccc
     2221 tccatataac atgaatttta caatagcgaa aaagaaagaa caatcaaggg tccccaaact
     2281 caccctgaag ttctcaggat cggtcgaccg gaggctggat cggtcccggt gtcttctatg
     2341 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct
     2401 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     2461 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     2521 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     2581 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     2641 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     2701 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     2761 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     2821 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     2881 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     2941 aattgattac tattaataac tagtcaataa tcaatgtcaa catggcggta atgttggaca
     3001 tgagccaata taaatgtaca tattatgata tggatacaac gtatgcaatg ggccaagctc
     3061 tctagagggc cgcgatccgc cgtccctaac ccacggggcc cgtggctatg gcagggcttg
     3121 ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg
     3181 aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt
     3241 gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat
     3301 tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt
     3361 cagttagcct cccccatctc ccggggtggg cgaagaactc cagcatgaga tccccgcgct
     3421 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     3481 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     3541 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     3601 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     3661 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     3721 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     3781 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
     3841 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     3901 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     3961 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     4021 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     4081 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     4141 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     4201 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     4261 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     4321 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     4381 agatctgcgg cacgctgttg acgctgttaa gcgggtcgct gcagggtcgc tcggtgttcg
     4441 aggccacacg cgtcacctta atatgcgaag tggacctggg accgcgccgc cccgactgca
     4501 tctgcgtgtt cgaattcgcc aatgacaaga cgctgggcgg ggtttgtgtc atcatagaac
     4561 taaagacatg caaatatatt tcttccgggg acaccgccag caaacgcgag caacgggcca
     4621 cggggatgaa gcaggatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     4681 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     4741 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
     4801 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     4861 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     4921 agcccttgcg ccctgagtgc ttgcggcagc gtgaagcttg gcactggccg tcgttttaca
     4981 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     5041 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5101 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5161 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5221 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5281 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5341 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5401 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5461 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5521 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5581 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5641 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5701 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5761 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5821 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5881 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5941 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6001 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6061 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6121 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6181 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6241 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6301 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6361 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6421 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6481 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6541 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6721 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7261 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7321 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7381 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7441 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7501 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7561 cacaggaaac agctatgaca tgattacgaa ttgcggccga tccgtcgagg gatctccata
     7621 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     7681 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     7741 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     7801 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     7861 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     7921 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     7981 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     8041 tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc acaccagcca
     8101 ccaccttctg ataggcagcc tgcacctgag gagtgaattc ctcgacggat catcaaccac
     8161 tttgtacaag aaagctgggt ctagatatct cgagtgcggc cgctcatcgc gacgtgctgg
     8221 ctggggcctg gggggagaaa gccggcgggg gctcagggaa aggcgcggcc gggctcccga
     8281 agcccgtccc tgcagcctgc cccagtgccc tcctccgctg cccgcgcgcc cgccgggccc
     8341 tcaccagcgt gaaggcgtcg taggcctgag ccagctcctc agtgcggtac tgctccagca
     8401 ccaccacgcc ctgcaggcct gcgctgcggc gccgggcaca gccctcgcag tgtaccaggt
     8461 acgtgttgcg gctgccattc tcacttgtca cgaacaggat gttaaacacc tccacatcgc
     8521 actcgttgca gtagtaggct ggctcgtcct tgacacggcc ctggtaagcg attttcttcc
     8581 ctgcccgcac caggctctcg cgttgcacct ggcagtgctt catggactgc agcaggcaga
     8641 acttgatcat cttgaacaag tcggggtcgc tgattttgac cgtgcgagcc acgttccatg
     8701 acacgtgaat catgggcacg atggatttga cgttcttcac ctcattccac tcgtatcgtt
     8761 ccagggccag ctggtactga taggcggtga ggggccccac gttccaggca atgttgttgc
     8821 accagccggt ggcctgcacc cagtgcacag tccccgcatt aatccacacg aggtctccgg
     8881 gtcgctgcac gaagcggtac acaggaatat tggatgcata gagatcatcc aggattggcc
     8941 accaggaacc cgtcaagtag tccacgccgt gccgatcaca gaaagcgctg atggtctccc
     9001 agtagtgctc gtgcaccgcg aaccactcgc agtcgcctgg gccaatgttg atgttgacgg
     9061 agcagaagtt gttattctcc tggtggcctg gcgttcggct gccgggcacc ttcatgtaca
     9121 gctgcaccgt gttcatgccc aggatggtgt ggcccacgtg gctcagcatg ttgcccgtgg
     9181 atgttacccg catgaaggcg ggcagcttca gcagctcctg cagctggggc ttccaccgct
     9241 tagcatcaga caagtcgatg ttggtgccaa acttgatgat gtgatggttc ttcgggtctg
     9301 gtgcgctgct aggaggggtt ccagtggtgc tgtctggctc ctctgactcc tcatcctcac
     9361 tctccttctc ctcctgcaga gactcctgga aggatgaggc ctggtactgt gcgtacttgg
     9421 caatggtggt gtgggaacgg gagctctcac aaggccagat ctgccgagtg cctgtcagat
     9481 cccagttctc atctgagggc tgctgcacct gggtgcgaac ttccacggtg tgttcgccac
     9541 tcgcttccac cagggtcttg gtggagaaga ggcccaagtt gagccgcagg gagcccgcca
     9601 ggccccggat cactgtgatg ggatttcgag ggtctgtaca gaactgcagc aggacaggtg
     9661 agaaggcatc ccgtttgctc tccagataga tgctgggtgt aggggggttg agtttttccc
     9721 ggggcagctt ctcctcagtg ttgatcttcg gtttcacaga ctgggcaggg gaaaggtagg
     9781 actctcgaaa cttccctttc accttggcat tcctgctggc gcgcacgacg tcagcagcac
     9841 agtggctgat ggtgaggtct gccatgcgca gccgccgctc ttccacctca gaggcgataa
     9901 aggtctcctt gtcaccactc tctaccttga tgagccggat cttcagctcc ttggggggcc
     9961 cctcactaag tgagcgcagc ttcagcatgt cggcccggct gacacccggt ggccctgggg
    10021 cttcctcccg agccttcttt ccagggacag aggctgacgg gggtgtgggc tgggcagaag
    10081 gtgcaggagc tgatggaggg gctggagctg tgggtggctt ggccttggag gccccgccaa
    10141 gatcgggccg ggacttctca cgaccctgga tctcctcgct ctgcaggtcc aggttcccca
    10201 gcacccggcg gctcttttct ttggggacct tggccttggc ctttgccctg ccctcacggg
    10261 gccgacgacc cacactgtcc ttacaggccc gcctgtgccg ccgatgctcc ttctgatgct
    10321 ccttctgtcg ccgcttacag ctgcctgaca ctgccgccac cccgccagcc tcctccttgg
    10381 cctgtgcggg gggcagcagt cgctcagtcc cactgtccgc tggctctgct gtgtccactg
    10441 ggtcggctgg gtcagtggca ctccggccca cccgctccac aagggtctcg caagcccggc
    10501 tgatctcttc tagcacctca gactcagagc gagcaggggg cagagatagg ggtgggggcg
    10561 gttgggtggg ggtcatgggg cccgggactg gctcttcgcc cgccctgcgc tcccgggccc
    10621 agggcccgcc tgaggtagag aactgagatg acgaggaagc agagggctgt ggggagccct
    10681 gggcgctagg ggccgctgag gtgggcggca gggcggtagc acctgatggg gggccagggg
    10741 aatactgagt ggtgggcaag ggcccaggcc gggtggaaac agctgctcca gtcccccgat
    10801 aacagtactt ttgtccctcc agcacggagg ccaaggattt gagcaggctg gccgggctgg
    10861 ggggtggggg tggtggtggc tgtggctgag agggtggagg gaacttggct agaggcggtg
    10921 gtggtggtag ggctggtggt ggcttcttct cctcttcctg ggtggccgtg gtggtggtgg
    10981 tggtggtggt ggtggtggtg acggcgacag caggagcagt agtggtgggg gctgtgtcgg
    11041 tggggaaagg agactgcaga gatgcaaagg gctccttcag cgggggttgg ggggccacgc
    11101 ctgcttcgtg ttgttgctgt tcctcttcct tgcgaatgga ttcgtccagc atcttcatga
    11161 tgttggccag cccatcaggt aggatcttga attcggctgg ctcctcaaac tggtcctcca
    11221 gcggagtggg gggaaaatca aagagtcgag ggcctcgggc aggcagctcc ccaaccccgg
    11281 gcggcacagg ctggggggct ttactcaggg ggcctggggg tggccccggc cccacctcgg
    11341 gggtctttgg gaaggagacc ctgggccggg ggctctccgg gcgcctgaag cttcctgagg
    11401 tattttggtg gcaggaggaa ggaggggctg gaggcagggc aagagtcagg ggtacaagag
    11461 gtgggtcctg tgggttctgt gctgggctgg gaggccgggg aagggggtct atacttctgg
    11521 ccagataggg tggtggggga aaggacacag gcccagcagg gctgctgctg tggctgccac
    11581 tgttgctgtt gctactgctg gtggttgggg ttgggggagt gggaggggtg tgagaatcct
    11641 gagtgcccac agaaaagcgg gaggccggag gccccaagaa gccctcgcga tgggggaggg
    11701 gtggtggggc agggcggggg ggtccctcag tc
//
