LOCUS       sequence-364536-        9414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression 
            (PhCMV-NLS-dCas9-3xNLS-pA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Haellman V, Strittmatter T, Bertschi A, Stucheli P, Fussenegger M
  TITLE     A versatile plasmid architecture for mammalian synthetic biology 
            (VAMSyB).
  JOURNAL   Metab Eng. 2021 Jul;66:41-50. doi: 10.1016/j.ymben.2021.04.003. Epub
            2021 Apr 18.
  PUBMED    33857582
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2021-07"; volume: "66:41-50. doi"; pages: " 
            10.1016/j.ymben.2021.04.003. Epub 2021 Apr 18"
COMMENT     Sequence Label: pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS
FEATURES             Location/Qualifiers
     source          1..9414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(128..716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(208..227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(887..1678,1679..1747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1510..1529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1748..1852)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1923..2302
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2303..2506
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2457..2477
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2551..2570
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2551..2569
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2619..2639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2646..2669,2670..2672,2673..5159,5160..5162,
                     5163..6746)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6765..6785
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6789..6809
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6813..6833
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6888..6905)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6894..7118
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7164..7592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7251..7270)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7461..7482
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7601..7621)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7606..7935
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7786..7921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7848..7867
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8002..8796
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8056..8075)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8666..8685
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8970..9091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9007..9026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9061..9080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9140..9156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9140..9156)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9153..9175)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9164..9180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9188..9194,9195..9212,9213..9218))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9233..9254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9371..9388)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
       61 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      121 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      541 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      661 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1321 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1861 cccgaaaagt gccacctgac gtcgacggat cgggagatct ccacgcgtgg taccctcgag
     1921 ttgacattga ttattgagta gttattaata gtaatcaatt acggggtcat tagttcatag
     1981 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2041 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2101 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2161 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2221 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2281 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2341 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2401 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2461 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     2521 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     2581 tgttcgaagc ggaattcacc atgactagtg gatctgatcc aaaaaagaag agaaaggtag
     2641 ctagtgacaa gaagtattct atcggactgg ccatcgggac taatagcgtc gggtgggccg
     2701 tgatcactga cgagtacaag gtgccctcta agaagttcaa ggtgctcggg aacaccgacc
     2761 ggcattccat caagaaaaat ctgatcggag ctctcctctt tgattcaggg gagaccgctg
     2821 aagcaacccg cctcaagcgg actgctagac ggcggtacac caggaggaag aaccggattt
     2881 gttaccttca agagatattc tccaacgaaa tggcaaaggt cgacgacagc ttcttccata
     2941 ggctggaaga atcattcctc gtggaagagg ataagaagca tgaacggcat cccatcttcg
     3001 gtaatatcgt cgacgaggtg gcctatcacg agaaataccc aaccatctac catcttcgca
     3061 aaaagctggt ggactcaacc gacaaggcag acctccggct tatctacctg gccctggccc
     3121 acatgatcaa gttcagaggc cacttcctga tcgagggcga cctcaatcct gacaatagcg
     3181 atgtggataa actgttcatc cagctggtgc agacttacaa ccagctcttt gaagagaacc
     3241 ccatcaatgc aagcggagtc gatgccaagg ccattctgtc agcccggctg tcaaagagcc
     3301 gcagacttga gaatcttatc gctcagctgc cgggtgaaaa gaaaaatgga ctgttcggga
     3361 acctgattgc tctttcactt gggctgactc ccaatttcaa gtctaatttc gacctggcag
     3421 aggatgccaa gctgcaactg tccaaggaca cctatgatga cgatctcgac aacctcctgg
     3481 cccagatcgg tgaccaatac gccgaccttt tccttgctgc taagaatctt tctgacgcca
     3541 tcctgctgtc tgacattctc cgcgtgaaca ctgaaatcac caaggcccct ctttcagctt
     3601 caatgattaa gcggtatgat gagcaccacc aggacctgac cctgcttaag gcactcgtcc
     3661 ggcagcagct tccggagaag tacaaggaaa tcttctttga ccagtcaaag aatggatacg
     3721 ccggctacat cgacggaggt gcctcccaag aggaatttta taagtttatc aaacctatcc
     3781 ttgagaagat ggacggcacc gaagagctcc tcgtgaaact gaatcgggag gatctgctgc
     3841 ggaagcagcg cactttcgac aatgggagca ttccccacca gatccatctt ggggagcttc
     3901 acgccatcct tcggcgccaa gaggacttct acccctttct taaggacaac agggagaaga
     3961 ttgagaaaat tctcactttc cgcatcccct actacgtggg acccctcgcc agaggaaata
     4021 gccggtttgc ttggatgacc agaaagtcag aagaaactat cactccctgg aacttcgaag
     4081 aggtggtgga caagggagcc agcgctcagt cattcatcga acggatgact aacttcgata
     4141 agaacctccc caatgagaag gtcctgccga aacattccct gctctacgag tactttaccg
     4201 tgtacaacga gctgaccaag gtgaaatatg tcaccgaagg gatgaggaag cccgcattcc
     4261 tgtcaggcga acaaaagaag gcaattgtgg accttctgtt caagaccaat agaaaggtga
     4321 ccgtgaagca gctgaaggag gactatttca agaaaattga atgcttcgac tctgtggaga
     4381 ttagcggggt cgaagatcgg ttcaacgcaa gcctgggtac ctaccatgat ctgcttaaga
     4441 tcatcaagga caaggatttt ctggacaatg aggagaacga ggacatcctt gaggacattg
     4501 tcctgactct cactctgttc gaggaccggg aaatgatcga ggagaggctt aagacctacg
     4561 cccatctgtt cgacgataaa gtgatgaagc aacttaaacg gagaagatat accggatggg
     4621 gacgccttag ccgcaaactc atcaacggaa tccgggacaa acagagcgga aagaccattc
     4681 ttgatttcct taagagcgac ggattcgcta atcgcaactt catgcaactt atccatgatg
     4741 attccctgac ctttaaggag gacatccaga aggcccaagt gtctggacaa ggtgactcac
     4801 tgcacgagca tatcgcaaat ctggctggtt cacccgctat taagaagggt attctccaga
     4861 ccgtgaaagt cgtggacgag ctggtcaagg tgatgggtcg ccataaacca gagaacattg
     4921 tcatcgagat ggccagggaa aaccagacta cccagaaggg acagaagaac agcagggagc
     4981 ggatgaaaag aattgaggaa gggattaagg agctcgggtc acagatcctt aaagagcacc
     5041 cggtggaaaa cacccagctt cagaatgaga agctctatct gtactacctt caaaatggac
     5101 gcgatatgta tgtggaccaa gagcttgata tcaacaggct ctcagactac gacgtggacg
     5161 ccatcgtccc tcagagcttc ctcaaagacg actcaattga caataaggtg ctgactcgct
     5221 cagacaagaa ccggggaaag tcagataacg tgccctcaga ggaagtcgtg aaaaagatga
     5281 agaactattg gcgccagctt ctgaacgcaa agctgatcac tcagcggaag ttcgacaatc
     5341 tcactaaggc tgagaggggc ggactgagcg aactggacaa agcaggattc attaaacggc
     5401 aacttgtgga gactcggcag attactaaac atgtcgccca aatccttgac tcacgcatga
     5461 ataccaagta cgacgaaaac gacaaactta tccgcgaggt gaaggtgatt accctgaagt
     5521 ccaagctggt cagcgatttc agaaaggact ttcaattcta caaagtgcgg gagatcaata
     5581 actatcatca tgctcatgac gcatatctga atgccgtggt gggaaccgcc ctgatcaaga
     5641 agtacccaaa gctggaaagc gagttcgtgt acggagacta caaggtctac gacgtgcgca
     5701 agatgattgc caaatctgag caggagatcg gaaaggccac cgcaaagtac ttcttctaca
     5761 gcaacatcat gaatttcttc aagaccgaaa tcacccttgc aaacggtgag atccggaaga
     5821 ggccgctcat cgagactaat ggggagactg gcgaaatcgt gtgggacaag ggcagagatt
     5881 tcgctaccgt gcgcaaagtg ctttctatgc ctcaagtgaa catcgtgaag aaaaccgagg
     5941 tgcaaaccgg aggcttttct aaggaatcaa tcctccccaa gcgcaactcc gacaagctca
     6001 ttgcaaggaa gaaggattgg gaccctaaga agtacggcgg attcgattca ccaactgtgg
     6061 cttattctgt cctggtcgtg gctaaggtgg aaaaaggaaa gtctaagaag ctcaagagcg
     6121 tgaaggaact gctgggtatc accattatgg agcgcagctc cttcgagaag aacccaattg
     6181 actttctcga agccaaaggt tacaaggaag tcaagaagga ccttatcatc aagctcccaa
     6241 agtatagcct gttcgaactg gagaatgggc ggaagcggat gctcgcctcc gctggcgaac
     6301 ttcagaaggg taatgagctg gctctcccct ccaagtacgt gaatttcctc taccttgcaa
     6361 gccattacga gaagctgaag gggagccccg aggacaacga gcaaaagcaa ctgtttgtgg
     6421 agcagcataa gcattatctg gacgagatca ttgagcagat ttccgagttt tctaaacgcg
     6481 tcattctcgc tgatgccaac ctcgataaag tccttagcgc atacaataag cacagagaca
     6541 aaccaattcg ggagcaggct gagaatatca tccacctgtt caccctcacc aatcttggtg
     6601 cccctgccgc attcaagtac ttcgacacca ccatcgaccg gaaacgctat acctccacca
     6661 aagaagtgct ggacgccacc ctcatccacc agagcatcac cggactttac gaaactcgga
     6721 ttgacctctc acagctcgga ggggatgagg gagctgctag tgatccaaaa aagaagagaa
     6781 aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagctagcg
     6841 gatccaccgg tgtctagaaa gctttgaggc cggcctgcag ggatcagcct cgactgtgcc
     6901 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6961 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7021 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7081 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7141 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7201 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7261 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7321 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7381 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7501 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7561 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7681 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7741 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7861 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7921 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     7981 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     8041 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8101 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8161 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     8221 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8281 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     8341 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8401 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     8461 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     8521 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     8581 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     8641 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     8701 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     8761 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     8821 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     8881 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8941 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     9001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     9121 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9181 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9241 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9301 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9361 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgag
//
