LOCUS       sequence-364551-        3547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 NSP7 fused to BioID2 and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL,
            Halfmann CT, Diaz Pena R, Pratt D, Campos AR, Roux KJ
  TITLE     A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins.
  JOURNAL   Viruses. 2022 Mar 15;14(3). pii: v14030611. doi: 10.3390/v14030611.
  PUBMED    35337019
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses."; 
            date: "2022-03-15"; volume: "14(3). pii: v14030611. doi"; pages: " 
            10.3390/v14030611"
COMMENT     Sequence Label: NSP7-1x-BioID2 pBabe-puro (SARS-CoV-2)
FEATURES             Location/Qualifiers
     source          1..3547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      94..229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             253..852
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(253..272)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     749..769
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             951..1544
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1607..1806
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2007..2423
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2353..2375
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2391..2407
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             2719..3414
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             3415..3441
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3456..3476)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(3461..3547,1..243)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
       61 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      121 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      181 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      241 aaaaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      301 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
      361 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
      421 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
      481 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
      541 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
      601 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
      661 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
      721 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
      781 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
      841 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
      901 catgcatcga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
      961 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     1021 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     1081 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     1141 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     1201 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     1261 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     1321 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     1381 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     1441 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     1501 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
     1561 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggcaagc tggccagcaa
     1621 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
     1681 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
     1741 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
     1801 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
     1861 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
     1921 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
     1981 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     2041 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     2101 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     2161 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     2221 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     2281 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     2341 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     2401 ccctcactcc ttctctaggc gccggccgga tccgccacca tgagcaagat gtccgacgtg
     2461 aagtgcacct ccgtggtgct gctgtctgtg ctgcagcagc tgcgcgtgga gagctcctct
     2521 aagctgtggg cccagtgcgt gcagctgcac aacgacatcc tgctggccaa ggacaccacc
     2581 gaggctttcg aaaagatggt gtctctgctg agcgtgctgc tgtccatgca gggagctgtg
     2641 gacatcaaca agctgtgcga ggaaatgctg gacaacaggg ctaccctgca gaccggtggt
     2701 ggaggcgggt ctgaattctt caagaacctg atctggctga aggaggtgga cagcacccag
     2761 gagagactga aggagtggaa cgtgagctac ggcaccgccc tggtggccga cagacagacc
     2821 aagggcagag gcggcctggg cagaaagtgg ctgagccagg agggcggcct gtacttcagc
     2881 ttcctgctga accccaagga gttcgagaac ctgctgcagc tgcccctggt gctgggcctg
     2941 agcgtgagcg aggccctgga ggagatcacc gagatcccct tcagcctgaa gtggcccaac
     3001 gacgtgtact tccaggagaa gaaggtgagc ggcgtgctgt gcgagctgag caaggacaag
     3061 ctgatcgtgg gcatcggcat caacgtgaac cagagagaga tccccgagga gatcaaggac
     3121 agagccacca ccctgtacga gatcaccggc aaggactggg acagaaagga ggtgctgctg
     3181 aaggtgctga agagaatcag cgagaacctg aagaagttca aggagaagag cttcaaggag
     3241 ttcaagggca agatcgagag caagatgctg tacctgggcg aggaggtgaa gctgctgggc
     3301 gagggcaaga tcaccggcaa gctggtgggc ctgagcgaga agggcggcgc cctgatcctg
     3361 accgaggagg gcatcaagga gatcctgagc ggcgagttca gcctgagaag aagctaccct
     3421 tacgatgtac cggattacgc atgagtcgac cctgtggaat gtgtgtcagt tagggtgtgg
     3481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3541 aaccagg
//
