LOCUS       sequence-364565-        6546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myc tagged Miro1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Fransson A, Ruusala A, Aspenstrom P
  TITLE     Atypical Rho GTPases have roles in mitochondrial homeostasis and 
            apoptosis.
  JOURNAL   J Biol Chem. 2003 Feb 21;278(8):6495-502. Epub 2002 Dec 12.
  PUBMED    12482879
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2003-02-21"; volume: "278(8)"; pages: "6495-502. Epub
            2002 Dec 12"
COMMENT     Sequence Label: pRK5-myc-Miro1
FEATURES             Location/Qualifiers
     source          1..6546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        97..454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        97..288
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(120..140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      305..440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(474..491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(474..490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(483..505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      703..1158
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(790..809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1000..1021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1175..1194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1294..1316
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1354..1372)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1440..1544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1545..1613,1614..2405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1763..1782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2576..3164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3065..3084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3318..3335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3452..3473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3488..3493,3494..3511,3512..3518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3526..3542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3531..3553
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3550..3566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3550..3566
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        3595..3974
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3975..4178
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4129..4149
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4175..4199
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4409..4427
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4409..4426
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     4440..4459
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      4506..4515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4515..4544
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      6427..6436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    join(6440..6546,1..28)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6477..6496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     join(6531..6546,1..4)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 catcaatgta tcttatcatg tctggatcgg gaattaattc ggcgcagcac catggcctga
       61 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
      121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      361 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      421 aggaggcttt tttggaggcc taggcttttg caaaaagctg ttaacagctt ggcactggcc
      481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      601 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      661 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
      721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      841 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      901 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      961 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     1021 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     1081 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     1141 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     1201 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     1261 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     1321 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     1381 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1441 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1501 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1561 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1621 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1681 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1741 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1801 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1861 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1921 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1981 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2041 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2101 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2161 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2221 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2281 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2341 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2401 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2701 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3241 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3361 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3421 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3481 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3541 aatttcacac aggaaacagc tatgacatga ttacgaatta attcgagctc gcccgacatt
     3601 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3661 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3721 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3781 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     3841 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3901 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3961 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4021 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4081 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4141 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     4201 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     4261 tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta
     4321 ccgcctatag agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat
     4381 acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt
     4441 tgcctttctc tccacaggtg tccactccca ggtccaactg cacctcggtt ctatcgattg
     4501 aattggccac catggagcag aagctgatct ccgaggagga cctgggatct atgaagaaag
     4561 acgtgcggat cctgctggtg ggagaaccta gagttgggaa gacatcactg attatgtctc
     4621 tggtcagtga agaatttcca gaagaggttc ctccccgggc agaagaaatc accattccag
     4681 ctgatgtcac cccagagaga gttccaacac acattgtaga ttactcagaa gcagaacaga
     4741 gtgatgaaca acttcatcaa gaaatatctc aggctaatgt catctgtata gtgtatgccg
     4801 ttaacaacaa gcattctatt gataaggtaa caagtcgatg gattcctctc ataaatgaaa
     4861 gaacagacaa agacagcagg ctgcctttaa tattggttgg gaacaaatct gatctggtgg
     4921 aatatagtag tatggagacc atccttccta ttatgaacca gtatacagaa atagaaacct
     4981 gtgtggagtg ttcagcgaaa aacctgaaga acatatcaga gctcttttat tacgcacaga
     5041 aagctgttct tcatcctaca gggcccctgt actgcccaga ggagaaggag atgaaaccag
     5101 cttgtataaa agcccttact cgtatattta aaatatctga tcaagataat gatggtactc
     5161 tcaatgatgc tgaactcaac ttctttcaga ggatttgttt caacactcca ttagctcctc
     5221 aagctctgga ggatgtcaag aatgtagtca gaaaacatat aagtgatggt gtggctgaca
     5281 gtgggttgac cctgaaaggt tttctctttt tacacacact ttttatccag agagggagac
     5341 acgaaactac ttggactgtg cttcgacgat ttggttatga tgatgacctg gatttgacac
     5401 ctgaatattt gttccccctg ctgaaaatac ctcctgattg cactactgaa ttaaatcatc
     5461 atgcatattt atttctccaa agcacctttg acaagcatga tttggataga gactgtgctt
     5521 tgtcacctga tgagcttaaa gatttattta aagttttccc ttacatacct tgggggccag
     5581 atgtgaataa cacagtttgt accaatgaaa gaggctggat aacctaccag ggattccttt
     5641 cccagtggac gctcacgact tatttagatg tacagcggtg cctggaatat ttgggctatc
     5701 taggctattc aatattgact gagcaagagt ctcaagcttc agctgttaca gtgacaagag
     5761 ataaaaagat agacctgcag aaaaaacaaa ctcaaagaaa tgtgttcaga tgtaatgtaa
     5821 ttggagtgaa aaactgtggg aaaagtggag ttcttcaggc tcttcttgga agaaacttaa
     5881 tgaggcagaa gaaaattcgt gaagatcata aatcctacta tgcgattaac actgtttatg
     5941 tatatggaca agagaaatac ttgttgttgc atgatatctc agaatcggaa tttctaactg
     6001 aagctgaaat catttgtgat gttgtatgcc tggtatatga tgtcagcaat cccaaatcct
     6061 ttgaatactg tgccaggatt tttaagcaac actttatgga cagcagaata ccttgcttaa
     6121 tcgtagctgc aaagtcagac ctgcatgaag ttaaacaaga atacagtatt tcacctactg
     6181 atttctgcag gaaacacaaa atgcctccac cacaagcctt cacttgcaat actgctgatg
     6241 cccccagtaa ggatatcttt gttaaattga caacaatggc catgtatccg cacgtgacac
     6301 aagctgacct caagagctcc acgttttggc ttcgagcaag ttttggtgct actgtttttg
     6361 cagttttggg ctttgctatg tacaaagcat tattgaaaca gcgatgagaa ttcctgcaga
     6421 agcttggccg ccatggccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6541 caaact
//
