LOCUS       sequence-364593-        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mos1 transposase expression vector for use in creating targeted 
            transgene insertions and targeted deletions in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ601 - Peft-3 Mos1 transposase
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(82..873,874..942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     705..724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(943..1047)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1115..1133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1171..1193)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1506..1528
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1520..1537
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1521..1537
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1545..1565
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(1569..1892)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    1897..1921
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3014..3038)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3047..3646)
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    complement(3647..3667)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3687..3703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3687..3703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3700..3722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(3849..4437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3929..3948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
       61 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      121 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      181 ggagggctta ccatctgacc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      241 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
      301 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
      361 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
      421 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
      481 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
      541 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
      601 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
      661 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
      721 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
      781 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
      841 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
      901 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
      961 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1021 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1081 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1141 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1201 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1261 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg cactgagagt
     1321 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     1381 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     1441 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     1501 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     1561 agttgtgaga cttttttctt ggcggcacaa taaagtttcc tctttttgtt gggtcactca
     1621 acgaacacaa tacaaatatt tgagcgctcg catttttctc aacctttcgg gtttaaagca
     1681 cttcaaaatg gaattgaaga gagattgtga aagtacatca gacagaatca cattttgata
     1741 gcattcactt cactcagatg caaacttttc agtgactata aaaagtcata aaataataac
     1801 attaaaaaag caaataaatt agcgagagaa ttttttgaca aaaagaaaga agagtgatag
     1861 agaagaaggg aatgcttgaa aggattttgc atttatccca ctttgtacaa gaaagctggg
     1921 tactattcaa agtatttgcc gtcgctcgcg acacattttt cccatctctc gggcaatttg
     1981 tggattccac gccagtagaa ctcatcgtct tttgcggcga accattcatc gagccatttt
     2041 ttcacacttt cgtaagaatc gaagcgctgc tcagcgagtg cgtgtcccat cgaagcgaat
     2101 aggtggtaat cggatggggc caggtctggt gagtaagccg catgcggaag cacttcccaa
     2161 ttgagtgttt ccaacgtgtc gcgaaccgct cttgccgtat gtgatggagc gttgtcatgg
     2221 agaaaaatga ccctgtgttg tcttttttga tattccggtc gttttctctg aagcgcacgg
     2281 ttcaaattga tcaattgttg ttggtagcgt gccgtattca ccgtttcgcc gggtttcaag
     2341 agctcatagt aaatgacacc gctctgatcc caccaaacac agagcatcgt cttcttgcca
     2401 aagcgattcg gtcgagcagt cgatgtggcc ggttgtccag gatcaacgta tgacttttta
     2461 cgtttaggat taacaaaaaa gatccatttt tcatctccag taacgatacg atgcaaaaac
     2521 gacttccttt tgtatcgtga aagcaaaatt tcgcatgtgt ttttgcgcct ctccatctgc
     2581 ctctcgttca actcatgtgg cacccatcta ccgaccttct gaatctttcc catctctcgc
     2641 aagcgattgg aaactgcttg ttgacttact tccaactgct ctgcgagttg tttttgcctg
     2701 aaaatttaaa taatccatgg gttagttagt atatatgttt aaacttacgt ttgagcatcg
     2761 tcttcatcca ataatgcttg cagttcggcg tcttcgtacc tttttggcgg ttttccgtgc
     2821 tctttgtcgt cgacgtcaaa atcaccactt ttgaagcgtt gaaaccaccg ttcacacgtt
     2881 ttcacagttg gtacttgttc gccaaaggct tcaacaagca ttcggtgcga ttccgcagct
     2941 gttttcttca aatgaaaaca gaaaattaat actgtccgcg tttgctcttt attcggcacg
     3001 aaactcgaca ttaagcctgc ttttttgtac aaacttgtga gcaaagtgtt tcccaactga
     3061 aaaaaaaaca atttaattta aaagtaagaa gagtgcggac ggtagagaga ataaaagtcg
     3121 aaaaaattat cctagaaact tctaatattt ttaaaaatta aaaaaatata aaaagtgagg
     3181 tcacaaataa gacagttaga acatttgttg aaagcctaaa ataaaaattc gactaaacag
     3241 tgagaacagg aaaaacgctg ggactgaatg tttgacctca ttataaattc ataaaattta
     3301 aatttcctaa tctggataaa aacaaagata aaaacaaaat aaaccaacaa aaaaccaaca
     3361 tgattagtca gatgaccaga aaactcacgt taatttcaca aggcccaaaa aaatctctcc
     3421 ccctctcgtt gctgcctgca catctaactc ctagcacgaa aatgtaccgt acaccatttc
     3481 aaacactctc ggcgggagtg ttgcatactt tttctctctg gcagtttatt ttcaataaat
     3541 tcttcatttt tatattgtat aatgtcttta ttttatgaaa aattcattaa tttaacagaa
     3601 acaatggaag aaccaatgga agttgacaat aaaagaccaa aggtgccaac ttttctatac
     3661 aaagttgata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3721 ctggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3781 ggaaagaaca tgtgagcaaa aggccagcaa aagaccagga accgtaaaaa ggccgcgttg
     3841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4261 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4501 attttggtca tgagattatc aaaaag
//
