LOCUS       sequence-364595-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA mediated knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Asimi V, Sampath Kumar A, Niskanen H, Riemenschneider C, Hetzel S, 
            Naderi J, Fasching N, Popitsch N, Du M, Kretzmer H, Smith ZD, 
            Weigert R, Walther M, Mamde S, Meierhofer D, Wittler L, Buschow R, 
            Timmermann B, Cisse II, Ameres SL, Meissner A, Hnisz D
  TITLE     Hijacking of transcriptional condensates by endogenous retroviruses.
  JOURNAL   Nat Genet. 2022 Aug;54(8):1238-1247. doi: 
            10.1038/s41588-022-01132-w. Epub 2022 Jul 21.
  PUBMED    35864192
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-022-01132-w"; journalName: "Nat Genet"; date: 
            "2022-08"; volume: "54"; issue: "8"; pages: "1238-1247"
COMMENT     Sequence Label: pLKO.1-Tet-Puro-shIAPEz_1
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      546..1134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1035..1054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1288..1305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1422..1443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1458..1463,1464..1481,1482..1488)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1496..1512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1501..1523
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1520..1536
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1520..1536
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1555..1575
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1557..1575
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1603..1829
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1830..2010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2057..2182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2675..2908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3093..3137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3426..3520
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     3437..3456
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    3474..3492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3502..3520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3621..3738
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3787..4297
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4216..4234
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          4306..4878
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4886..4905
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4933..4951
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4962..5582
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5035..5054)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5617..6190
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5784..5801)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6011..6030
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6210..6809
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6210..6229)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6706..6726
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6937..7170
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7242..7363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7279..7298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7333..7352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7403..7538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7559..7577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7587..7604)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7587..7603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7596..7618)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7745..8200
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7832..7851)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8042..8063
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8226..8330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8331..8399,8400..8816,1..375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8549..8568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
       61 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
      541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1261 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1321 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1381 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1441 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1501 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     1561 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     1621 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     1681 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     1741 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     1801 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     1861 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1921 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1981 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     2041 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     2101 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2161 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2221 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2281 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     2341 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     2401 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     2461 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     2521 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     2581 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     2641 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     2701 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     2761 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     2821 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     2881 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     2941 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3001 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3061 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3121 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3181 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3241 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     3301 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     3361 gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag cctcgagcgg
     3421 ccgcaatatt tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatccctat
     3481 cagtgataga gacttataag ttccctatca gtgatagaga caccggactg cgggtcgcgg
     3541 taataaactc gagtttatta ccgcgacccg cagttttttg aattctcgac ctcgagacaa
     3601 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     3661 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     3721 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg
     3781 ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     3841 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     3901 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     3961 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     4021 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     4081 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     4141 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     4201 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     4261 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc
     4321 cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt
     4381 ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa
     4441 ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca
     4501 ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact
     4561 tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag
     4621 ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa
     4681 ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac
     4741 aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt
     4801 gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc
     4861 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     4921 ggcaaagaat tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa
     4981 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     5041 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
     5101 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
     5161 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
     5221 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
     5281 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
     5341 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
     5401 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
     5461 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
     5521 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
     5581 ccgcgtacag cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccntaa
     5641 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5701 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5761 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5821 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5881 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5941 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     6001 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     6061 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     6121 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     6181 acgatgataa ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg
     6241 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6301 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     6361 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6421 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6481 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6541 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6601 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6661 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6721 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6781 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     6841 aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg
     6901 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6961 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7021 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7081 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7141 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7201 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7321 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7381 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7561 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7621 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7681 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7741 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7801 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7861 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7921 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7981 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8041 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8101 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8161 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8221 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8281 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8341 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8401 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8461 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8521 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8581 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8641 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8701 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8761 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgt
//
