LOCUS       sequence-364646-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Puromycin 
            selection, V5 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX302
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..510
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    635..752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        869..1172
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1173..1376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1327..1347
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1399..1523
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1548..1553,1554..1571,1572..1578)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1632..2291
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1698..1717)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2633..2938
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2817..2836
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2979..3103)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3105..3146
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3188..3776
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3241..3261)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3659..3670)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3848..4081
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4159..4280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4196..4215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4250..4269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4320..4455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4475..4494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4476..4494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4504..4521)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4504..4520)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4513..4535)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4662..5117
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4749..4768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4959..4980
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5143..5247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5248..5316,5317..6108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5466..5485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6279..6867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6768..6787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7021..7038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7155..7176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7191..7196,7197..7214,7215..7221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7229..7245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7234..7256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7253..7269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7253..7269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7288..7308
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7290..7308
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7336..7562
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7563..7743
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7790..7915
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8408..8641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8826..8870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9045..9545
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9464..9482
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(9567..9573,1..593)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(join(9567..9573,1..13))
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
       61 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
      121 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
      181 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
      241 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
      301 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
      361 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
      421 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
      481 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
      541 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagaattct
      601 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      661 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      721 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      781 cactttggcg ccggctcgag ggggcccggg gttattaata gtaatcaatt acggggtcat
      841 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      901 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      961 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1021 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1081 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1141 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     1201 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1261 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1321 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     1381 ggctaactgt cgggatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
     1441 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     1501 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
     1561 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
     1621 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     1681 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     1741 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     1801 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     1861 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     1921 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1981 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     2041 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     2101 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     2161 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     2221 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     2281 gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc
     2341 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     2401 gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     2461 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     2521 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     2581 cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt
     2641 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     2701 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     2761 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     2821 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     2881 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     2941 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     3001 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     3061 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttggtaag cctatcccta
     3121 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
     3181 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3241 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3301 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3361 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3421 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3481 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3541 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3601 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3661 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3721 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     3781 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     3841 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     3901 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3961 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4021 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4081 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4141 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4201 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4261 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     4321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4441 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     4501 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4561 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4621 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     4681 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4741 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4801 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4861 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4921 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4981 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5041 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5101 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5161 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5221 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5281 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5341 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5401 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5461 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5521 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5581 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5641 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5701 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5761 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5821 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5881 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5941 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6001 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6061 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6121 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6181 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6241 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6301 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6361 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6421 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6481 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6541 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6601 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6661 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6721 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6781 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6841 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6901 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6961 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7021 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7081 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7141 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7201 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7261 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     7321 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     7381 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     7441 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     7501 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     7561 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7621 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7681 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7741 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7801 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7861 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7921 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7981 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     8041 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     8101 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     8161 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     8221 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     8281 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     8341 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     8401 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     8461 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8521 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8581 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     8641 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     8701 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8761 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8821 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8881 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8941 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     9001 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     9061 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     9121 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     9181 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     9241 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     9301 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     9361 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     9421 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     9481 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     9541 cccagggggt accaccggag cttaccatga ccg
//
