LOCUS       sequence-364672-       13046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, 
            Hung T, Argani P, Rinn JL, Wang Y, Brzoska P, Kong B, Li R, West RB,
            van de Vijver MJ, Sukumar S, Chang HY
  TITLE     Long non-coding RNA HOTAIR reprograms chromatin state to promote 
            cancer metastasis.
  JOURNAL   Nature. 2010 Apr 15. 464(7291):1071-6.
  PUBMED    20393566
REFERENCE   2  (bases 1 to 13046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Apr 15. 464(7291):1071-6."
COMMENT     Sequence Label: LZRS-HOTAIR
FEATURES             Location/Qualifiers
     source          1..13046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             52..521
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    586..943
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1008..1424
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1354..1376
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1392..1408
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1434..1808
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1746..1762
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             4255..4724
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4854..4871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5025..5613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5105..5124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5736..5870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5760..5779)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5814..5833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6100..6699)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6183..6203)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6680..6699
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6782..7281)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6830..6849)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7223..7246
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      9313..11102
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(11596..12387,12388..12456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12219..12238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12457..12561)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cataactgag aatagaaaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
       61 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
      121 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
      181 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
      241 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
      301 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
      361 cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
      421 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
      481 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
      541 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      601 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
      661 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
      721 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
      781 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctta gaggagggat
      841 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
      901 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
      961 gtctctgtct gactgtgttt ctgtatttgt ctgaaaattt gggcccgggc cagactgtta
     1021 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
     1081 agtcggtagt tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
     1141 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
     1201 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
     1261 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
     1321 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
     1381 ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg
     1441 agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac ttgacaagag
     1501 ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag
     1561 tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc
     1621 cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc
     1681 gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca
     1741 tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct
     1801 ctagactgcc ggatccagac gctgcgtttg ctggctttga tgaaagactc gcctgtgctc
     1861 tggagcttga tccgaaagct tccacagtga ggactgctcc gtgggggtaa gagagcacca
     1921 ggcactgagg cctgggagtt ccacagacca acacccctgc tcctggcggc tcccacccgg
     1981 ggcttagacc ctcaggtccc taatatcccg gaggtgctct caatcagaaa ggtcctgctc
     2041 cgcttcgcag tggaatggaa cggatttaga agcctgcagt aggggagtgg ggagtggaga
     2101 gagggagccc agagttacag acggcggcga gaggaaggag gggcgtcttt atttttttaa
     2161 ggccccaaag agtctgatgt ttacaagacc agaaatgcca cggccgcgtc ctggcagaga
     2221 aaaggctgaa atggaggacc ggcgccttcc ttataagtat gcacattggc gagagaatta
     2281 agtgctgcaa cctaaaccag caattacacc caagctcgtt ggggcctaag ccagtaccga
     2341 cctggtagaa aaagcaacca cgaagctaga gagagagcca gaggagggaa gagagcgcca
     2401 gacgaaggtg aaagcgaacc acgcagagaa atgcaggcaa gggagcaagg cggcagttcc
     2461 cggaacaaac gtggcagagg gcaagacggg cactcacaga cagaggttta tgtattttta
     2521 ttttttaaaa tctgatttgg tgttccatga ggaaaaggga aaatctaggg aacgggagta
     2581 cagagagaat aatccgggtc ctagctcgcc acatgaacgc ccagagaacg ctggaaaaac
     2641 ctgagcgggt gccggggcag cacccggctc gggtcagcca ctgccccaca ccgggcccac
     2701 caagccccgc ccctcgcggc caccggggct tccttgctct tcttatcatc tccatcttta
     2761 tgatgaggct tgttaacaag accagagagc tggccaagca cctctatctc agccgcgccc
     2821 gctcagccga gcagcggtcg gtggggggac tgggaggcgc taattaattg attcctttgg
     2881 actgtaaaat atggcggcgt ctacacggaa cccatggact cataaacaat atatctgttg
     2941 ggcgtgagtg cactgtctct caaataattt ttccataggc aaatgtcaga gggttctgga
     3001 tttttagttg ctaaggaaag atccaaatgg gaccaatttt aggaggccca aacagagtcc
     3061 gttcagtgtc agaaaatgct tccccaaagg gttggcagtg tgttttgttg gaaaaaagct
     3121 tgggttatag gaaagccttt ccctgctact tgtgtagacc cagcccaatt taagaattac
     3181 aaggaagcga aggggttgtg taggccggaa gcctctctgt cccggctgga tgcaggggac
     3241 ttgagctgct ccggaatttg agaggaacat agaagcaaag gtccagcctt tgcttcgtgc
     3301 tgattcctag acttaagatt caaaaacaaa tttttaaaag tgaaaccagc cctagccttt
     3361 ggaagctctt gaaggttcag cacccaccca ggaatccacc tgcctgttac acgcctctcc
     3421 aagacacagt ggcaccgctt ttctaactgg cagcacagag caactctata atatgcttat
     3481 attaggtcta gaagaatgca tcttgagaca catgggtaac ctaattatat aatgcttgtt
     3541 ccatacagga gtgattatgc agtgggaccc tgctgcaaac gggactttgc actctaaata
     3601 taggccccag cttgggacaa aagttgcagt agaaaaatag acataggaga acacttaaat
     3661 aagtgatgca tgtagacaca gaaggggtat ttaaaagaca gaaataatag aagtacagaa
     3721 gaacagaaaa aaaatcagca gatggagatt accattccca atgcctgaac ttcctcctgc
     3781 tattaagatt gctagagaat tgtgtcttaa acagttcatg aacccagaag aacgcaattt
     3841 caatgtattt agtacacaca cagtatgtat ataaacacaa ctcacagaat atattttcca
     3901 tacattgggt aggtatgcac tttgtgtata tataataatg tattttccat gcagttttaa
     3961 aatgtagata tattaatatc tggatgcatt ttcaaaaaaa aaacctatag tggtcgtatt
     4021 aattcggatc cgcgaagggc gaattctgca gatatccatc acactggcgg ccgccagcac
     4081 agtggtcgac gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     4141 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     4201 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
     4261 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     4321 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     4381 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     4441 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     4501 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     4561 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     4621 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     4681 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca
     4741 aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga
     4801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5701 aggatcttca cctagatcct tttgcggccg aattagatcc agacatgata agatacattg
     5761 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5821 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     5881 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     5941 aaaacctcta caaatgtggt atggctgatt atgatcctct agagtcggtg ggcctcgggg
     6001 gcgggtgcgg ggtcggcggg gccgccccgg gtggcttcgg tcggagccat ggggtcgtgc
     6061 gctcctttcg gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt
     6121 catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag
     6181 ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc
     6241 gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg
     6301 gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc
     6361 caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg
     6421 cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc
     6481 caccgcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
     6541 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccgggtcga cggtgtggcg
     6601 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggcccggg ggacgtcgtc
     6661 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg gaaggtcgtc tccttgtgag
     6721 gggtcagggg cgtgggtcag gggatggtgg cggcaccggt cgtggcggcc gacctgcagg
     6781 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
     6841 tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc
     6901 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
     6961 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
     7021 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
     7081 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
     7141 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
     7201 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
     7261 ggaaaagcgc ctcccctacc cggtagaatt aattctcatg tttgacagct tatcatcgat
     7321 aagctgatcc tcacaggccg cacccagctt ttcttccgtt gccccagtag catctctgtc
     7381 tggtgacctt gaagaggaag aggaggggtc ccgagaatcc ccatccctac cgtccagcaa
     7441 aaagggggac gaggaatttg aggcctggct tgaggctcag gacgcaaatc ttgaggatgt
     7501 tcagcgggag ttttccgggc tgcgagtaat tggtgatgag gacgaggatg gttcggagga
     7561 tggggaattt tcagacctgg atctgtctga cagcgaccat gaaggggatg agggtggggg
     7621 ggctgttgga gggggcagga gtctgcactc cctgtattca ctgagcgtcg tctaataaag
     7681 atgtctattg atctctttta gtgtgaatca tgtctgacga ggggccaggt acaggacctg
     7741 gaaatggcct aggagagaag ggagacacat ctggaccaga aggctccggc ggcagtggac
     7801 ctcaaagaag agggggtgat aaccatggac gaggacgggg aagaggacga ggacgaggag
     7861 gcggaagacc aggagccccg ggcggctcag gatcagggcc aagacataga gatggtgtcc
     7921 ggagacccca aaaacgtcca agttgcattg gctgcaaagg gacccacggt ggaacaggag
     7981 caggagcagg agcgggaggg gcaggagcag gaggggcagg agcaggagga ggggcaggag
     8041 caggaggggs aggagsagga ggwggaggcs ggggtcgagg aggcagtgga ggccggggtc
     8101 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg
     8161 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa
     8221 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc
     8281 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc
     8341 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg
     8401 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc
     8461 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg
     8521 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg
     8581 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca
     8641 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc
     8701 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg
     8761 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga
     8821 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca
     8881 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg
     8941 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag
     9001 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc
     9061 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc
     9121 atctgtcttg tcaccatttt cgtcctccca acatggggca attgggcata cccatgttgt
     9181 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
     9241 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
     9301 gggagcaacc agcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
     9361 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
     9421 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
     9481 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     9541 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     9601 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
     9661 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
     9721 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
     9781 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     9841 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
     9901 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
     9961 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
    10021 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
    10081 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
    10141 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
    10201 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
    10261 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
    10321 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
    10381 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
    10441 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
    10501 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
    10561 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
    10621 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
    10681 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
    10741 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
    10801 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
    10861 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
    10921 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
    10981 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
    11041 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
    11101 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
    11161 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
    11221 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
    11281 ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca
    11341 ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc
    11401 ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac
    11461 caaccaactg aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc
    11521 aggtttagtt cgtccggggc ggggatctgg ccgcaaatca atctaaagta tatatgagta
    11581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccaccagctt tgctcttagg
    12601 agtttcctaa tacatcccaa actcaaatat ataaagcatt tgacttgttc tatgccctag
    12661 ggggcggggg gaagctaagc cagctttttt taacatttaa aatgttaatt ccattttaaa
    12721 tgcacagatg tttttatttc ataagggttt caatgtgcat gaatgctgca atattcctgt
    12781 taccaaagct agtataaata aaaatagata aacgtggaaa ttacttagag tttctgtcat
    12841 taacgtttcc ttcctcagtt gacaacataa atgcgctgct gagaagccag tttgcatctg
    12901 tcaggatcaa tttcccatta tgccagtcat attaattact agtcaattag ttgattttta
    12961 tttttgacat atacatgtga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
    13021 gccattttgc aaggcatgga aaaata
//
