LOCUS       sequence-364678-        9326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
  TITLE     RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
  PUBMED    23360965
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508."
COMMENT     Sequence Label: pCas9
FEATURES             Location/Qualifiers
     source          1..9326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     386..405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     602..619
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1011..1113
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1114..1773
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1180..1199)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3626..3645
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     repeat_region   4074..4109
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4140..4175
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(4176..4307)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     CDS             complement(4328..8434)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4331..8434)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        8730..8808
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        complement(join(9126..9131,9132..9148,9149..9154))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      join(9266..9326,1..485)
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
       61 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
      121 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
      181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
      241 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
      301 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
      361 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
      421 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
      481 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
      541 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
      601 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
      661 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
      721 acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac
      781 gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag
      841 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
      901 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
      961 cctgttgata ccgggaagcc ccgggccaac ttttggcgaa aatgagacgt tgatcggcac
     1021 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1081 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     1141 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     1201 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     1261 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1321 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1381 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1441 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1501 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1561 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1621 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1681 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1741 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1801 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     1861 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct
     1921 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
     1981 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt
     2041 attctgcctc ccagagcctg ataaaaacgg ttagcgcttc gttaatacag atgtaggtgt
     2101 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     2161 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgctgccgg
     2221 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     2281 gccccgcgcc caccggaagg agctaccggc agcggtgcgg actgttgtaa ctcagaataa
     2341 gaaatgaggc cgctcatggc gttgactctc agtcatagta tcgtggtatc accggttggt
     2401 tccactctct gttgcgggca acttcagcag cacgtagggg acttccgcgt ttccagactt
     2461 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     2521 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     2581 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
     2641 gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
     2701 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
     2761 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
     2821 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta
     2881 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca
     2941 gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct
     3001 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
     3061 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
     3121 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
     3181 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
     3241 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     3301 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     3361 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     3421 gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     3481 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     3541 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     3601 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     3661 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatccac aggacgggtg
     3721 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
     3781 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
     3841 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatcacac
     3901 tactcttctt ttgcctatta taacatgaaa acgctttaac agaaagaata ggaaggtatc
     3961 cgactgctgg tattaaccct ctttctcaag ttatcatcgg caatgttgaa tggagtccat
     4021 tcaaaacagc atagctctaa aacatggaac tcaacaagtc tcagtgtgct gaagttttgg
     4081 gaccattcaa aacagcatag ctctaaaacg agacctttga gcttccgaga ctggtctcag
     4141 ttttgggacc attcaaaaca gcatagctct aaaacctcgt agactatttt tgtctaaaaa
     4201 atttcgtaat cgcactattt gtctcagcta gacttcagtc ttgaaaagcc cctgtattac
     4261 tgcatttatt aagagtatta taccatattt ttagttatta agaaataatc ttcatctaaa
     4321 atatacttca gtcacctcct agctgactca aatcaatgcg tgtttcataa agaccagtga
     4381 tggattgatg gataagagtg gcatctaaaa cttcttttgt agacgtatat cgtttacgat
     4441 caattgttgt atcaaaatat ttaaaagcag cgggagctcc aagattcgtc aacgtaaata
     4501 aatgaataat attttctgct tgttcacgta ttggtttgtc tctatgtttg ttatatgcac
     4561 taagaacttt atctaaattg gcatctgcta aaataacacg cttagaaaat tcactgattt
     4621 gctcaataat ctcatctaaa taatgcttat gctgctccac aaacaattgt ttttgttcgt
     4681 tatcttctgg actacccttc aacttttcat aatgactagc taaatataaa aaattcacat
     4741 atttgcttgg cagagccagc tcatttcctt tttgtaattc tccggcacta gccagcatcc
     4801 gtttacgacc gttttctaac tcaaaaagac tatatttagg tagtttaatg attaagtctt
     4861 ttttaacttc cttatatcct ttagcttcta aaaagtcaat cggatttttt tcaaaggaac
     4921 ttctttccat aattgtgatc cctagtaact ctttaacgga ttttaacttc ttcgatttcc
     4981 ctttttccac cttagcaacc actaggactg aataagctac cgttggacta tcaaaaccac
     5041 catatttttt tggatcccag tcttttttac gagcaataag cttgtccgaa tttctttttg
     5101 gtaaaattga ctccttggag aatccgcctg tctgtacttc tgttttcttg acaatattga
     5161 cttggggcat ggacaatact ttgcgcactg tggcaaaatc tcgcccttta tcccagacaa
     5221 tttctccagt ttccccatta gtttcgatta gagggcgttt gcgaatctct ccatttgcaa
     5281 gtgtaatttc tgttttgaag aagttcatga tattagagta aaagaaatat tttgcggttg
     5341 ctttgcctat ttcttgctca gacttagcaa tcattttacg aacatcataa actttataat
     5401 caccatagac aaactccgat tcaagttttg gatatttctt aatcaaagca gttccaacga
     5461 cggcatttag atacgcatca tgggcatgat ggtaattgtt aatctcacgt actttataga
     5521 attggaaatc ttttcggaag tcagaaacta atttagattt taaggtaatc actttaacct
     5581 ctcgaataag tttatcattt tcatcgtatt tagtattcat gcgactatcc aaaatttgtg
     5641 ccacatgctt agtgatttgg cgagtttcaa ccaattggcg tttgataaaa ccagctttat
     5701 caagttcact caaacctcca cgttcagctt tcgttaaatt atcaaactta cgttgagtga
     5761 ttaacttggc gtttagaagt tgtctccaat agtttttcat ctttttgact acttcttcac
     5821 ttggaacgtt atccgattta ccacgatttt tatcagaacg cgttaagacc ttattgtcta
     5881 ttgaatcgtc tttaaggaaa ctttgtggaa caatgtgatc gacatcataa tcacttaaac
     5941 gattaatatc taattcttgg tccacataca tgtctcttcc attttggaga taatagagat
     6001 agagcttttc attttgcaat tgagtatttt caacaggatg ctctttaaga atctgacttc
     6061 ctaattcttt gataccttct tcgattcgtt tcatacgctc tcgcgaattt ttctggccct
     6121 tttgagttgt ctgattttca cgtgccattt caataacgat attttctggc ttatgccgcc
     6181 ccattacttt gaccaattca tcaacaactt ttacagtctg taaaatacct tttttaatag
     6241 cagggctacc agctaaattt gcaatatgtt catgtaaact atcgccttgt ccagacactt
     6301 gtgctttttg aatgtcttct ttaaatgtca aactatcatc atggatcagc tgcataaaat
     6361 tgcgattggc aaaaccatct gatttcaaaa aatctaatat tgttttgcca gattgcttat
     6421 ccctaatacc attaatcaat tttcgagaca aacgtcccca accagtataa cggcgacgtt
     6481 taagctgttt catcacctta tcatcaaaga ggtgagcata tgttttaagt ctttcctcaa
     6541 tcatctccct atcttcaaat aaggtcaatg ttaaaacaat atcctctaag atatcttcat
     6601 tttcttcatt atccaaaaaa tctttatctt taataatttt tagcaaatca tggtaggtac
     6661 ctaatgaagc attaaatcta tcttcaactc ctgaaatttc aacactatca aaacattcta
     6721 tttttttgaa ataatcttct tttaattgct taacggttac ttttcgattt gttttgaaga
     6781 gtaaatcaac aatggctttc ttctgttcac ctgaaagaaa tgctggtttt cgcattcctt
     6841 cagtaacata tttgaccttt gtcaattcgt tataaaccgt aaaatactca taaagcaaac
     6901 tatgttttgg tagtactttt tcatttggaa gatttttatc aaagtttgtc atgcgttcaa
     6961 taaatgattg agctgaagca cctttatcga caacttcttc aaaattccat ggggtaattg
     7021 tttcttcaga cttccgagtc atccatgcaa aacgactatt gccacgcgcc aatggaccaa
     7081 cataataagg aattcgaaaa gtcaagattt tttcaatctt ctcacgattg tcttttaaaa
     7141 atggataaaa gtcttcttgt cttctcaaaa tagcatgcag ctcacccaag tgaatttgat
     7201 ggggaataga gccgttgtca aaggtccgtt gcttgcgcag caaatcttca cgatttagtt
     7261 tcaccaataa ttcctcagta ccatccattt tttctaaaat tggtttgata aatttataaa
     7321 attcttcttg gctagctccc ccatcaatat aacctgcata tccgtttttt gattgatcaa
     7381 aaaagatttc tttatacttt tctggaagtt gttgtcgaac taaagctttt aaaagagtca
     7441 agtcttgatg atgttcatcg tagcgtttaa tcattgaagc tgatagggga gccttagtta
     7501 tttcagtatt tactcttagg atatctgaaa gtaaaatagc atctgataaa ttcttagctg
     7561 ccaaaaacaa atcagcatat tgatctccaa tttgcgccaa taaattatct aaatcatcat
     7621 cgtaagtatc ttttgaaagc tgtaatttag catcttctgc caaatcaaaa tttgatttaa
     7681 aattaggggt caaacccaat gacaaagcaa tgagattccc aaataagcca tttttcttct
     7741 caccggggag ctgagcaatg agattttcta atcgtcttga tttactcaat cgtgcagaaa
     7801 gaatcgcttt agcatctact ccacttgcgt taatagggtt ttcttcaaat aattgattgt
     7861 aggtttgtac caactggata aatagtttgt ccacatcact attatcagga tttaaatctc
     7921 cctcaatcaa aaaatgacca cgaaacttaa tcatatgcgc taaggccaaa tagattaagc
     7981 gcaaatccgc tttatcagta gaatctacca atttttttcg cagatgatag atagttggat
     8041 atttctcatg ataagcaact tcatctacta tatttccaaa aataggatga cgttcatgct
     8101 tcttgtcttc ttccaccaaa aaagactctt caagtcgatg aaagaaacta tcatctactt
     8161 tcgccatctc atttgaaaaa atctcctgta gataacaaat acgattcttc cgacgtgtat
     8221 accttctacg agctgtccgt ttgagacgag tcgcttccgc tgtctctcca ctgtcaaata
     8281 aaagagcccc tataagattt tttttgatac tgtggcggtc tgtatttccc agaaccttga
     8341 actttttaga cggaacctta tattcatcag tgatcaccgc ccatccgacg ctatttgtgc
     8401 cgatatctaa gcctattgag tatttcttat ccatttttgc ctcctaaaat aaaaagttta
     8461 aattaaatcc ataatgagtt tgatgatttc aataatagtt ttaatgacct ccgaaattag
     8521 tttaatatgc tttaattttt ctttttcaaa atatctcttc aaaaaatatt acccaatact
     8581 taataataaa tagattataa cacaaaattc ttttaaaaag tagtttattt tgttatcatt
     8641 ctatagtatt aagtattgtt ttatggctga taaatttctt tgaatttctc cttgattatt
     8701 tgttataaaa gttataaaat aatcttgttg gaaccattca aaacagcata gcaagttaaa
     8761 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttgatac
     8821 ttctattcta ctctgactgc aaaccaaaaa aacaagcgct ttcaaaacgc ttgttttatc
     8881 atttttaggg aaattaatct cttaatcctt ttatcattct acatttaggc gctgccatct
     8941 tgctaaacct actaagctcc acaggatgat ttcgtaatcc cgcaagaggc ccggcagtac
     9001 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
     9061 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
     9121 accgcattaa agcttatcga tgataagctg tcaaacatga gaattacaac ttatatcgta
     9181 tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt
     9241 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
     9301 ctgaaaacga aaaaaccgcc ttgcag
//
