LOCUS       sequence-36468-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Wirsing A, Johnstone KA, Harries LW, Ellard S, Ryffel GU, Stanik J, 
            Gasperikova D, Klimes I, Murphy R
  TITLE     Novel monogenic diabetes mutations in the P2 promoter of the HNF4A 
            gene are associated with impaired function in vitro.
  JOURNAL   Diabet Med. 2010 Jun . 27(6):631-5.
  PUBMED    20546279
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Diabet Med.
            2010 Jun . 27(6):631-5."
COMMENT     Sequence Label: HNF4A_P2-285/-136
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    405..496
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(437..456)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    510..558
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(826..847)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgccgggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa cagtaccgga
       61 atgccaagct tgggccaagc ccacccagcc ggagagctgg gggcatggag gagcaggggc
      121 ccgctcacag cagcagcaca gccaccagga gcccgtccac caggaaggcg gtgagtgcgg
      181 ccagctgcca ccgcccggtt atcttatcga ttcttctaat cacccaaggt gggtggatac
      241 gttaaagagt aaccagtcac ttagggaacc cgcggctggg gactgttgct gtctccatgg
      301 caatggcgac ctggccagac ctgaggtcca ctcgggtcct agtcctgcac tctggggccg
      361 ctcgagcccg ggctagcacg cgtaagagct cggtacctat cgatagagaa atgttctggc
      421 acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg ttttgttttg
      481 atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca gatgtaatga
      541 aaataaagat attttattgc ggccgctcca agtacctccc gtaccttaat attacttact
      601 tatcatggta gcttgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      661 cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt gttaaaattc
      721 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
      781 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      841 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaccgtc
//
