LOCUS       sequence-364688-        6891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector expressing GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Xiong W, MacColl Garfinkel AE, Li Y, Benowitz LI, Cepko CL
  TITLE     NRF2 promotes neuronal survival in neurodegeneration and acute nerve
            damage.
  JOURNAL   J Clin Invest. 2015 Apr;125(4):1433-45. doi: 10.1172/JCI79735. Epub 
            2015 Mar 23.
  PUBMED    25798616
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI79735"; 
            journalName: "J Clin Invest"; date: "2015-04"; volume: "125"; issue:
            "4"; pages: "1433-45"
COMMENT     Sequence Label: AAV-CMV-GFP
FEATURES             Location/Qualifiers
     source          1..6891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     590..614
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          707..1182
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      1260..1269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1266..1268,1269..1271,1272..1985)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1266..1268,1269..1271,1272..1982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1311..1332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1572..1591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1919..1940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2015..2031)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2016..2032)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2125..2519
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     repeat_region   2874..3003
                     /label=AAV2 ITR
     rep_origin      3374..3887
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3461..3480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3671..3692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4369..4388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4488..4510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4548..4566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4634..4738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4739..4807,4808..5599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4957..4976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5770..6358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6259..6278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6595..6724
                     /label=AAV2 ITR
     primer_bind     complement(6780..6799)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      601 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      661 gggaccgatc cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga
      721 cccttgatgt tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag
      781 aagtaacagg gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa
      841 atgctttctt cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct
      901 ctttctttca gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat
      961 aacagtgata atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca
     1021 tataaattgt aactgatgta agaggtttca tattgctaat agcagctaca atccagctac
     1081 cattctgctt ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc
     1141 cttttgctaa tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg
     1201 gtctgtgtgc tggcccatca ctttggcaaa gaattccgcg ggcccgggat ccaccggtcg
     1261 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1321 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1381 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1441 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1501 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1561 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1621 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1681 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1741 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1801 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1861 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1921 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1981 agtaaagcgg ccgctctaga ggatccaagc ttatcgatac cgtcgacctc gagggcccag
     2041 atctaattca ccccaccagt gcaggctgcc tatcagaaag tggtggctgg tgtggctaat
     2101 gccctggccc acaagtatca ctaagctcgc tttcttgctg tccaatttct attaaaggtt
     2161 cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct
     2221 ggattctgcc taataaaaaa catttatttt cattgcaatg atgtatttaa attatttctg
     2281 aatattttac taaaaaggga atgtgggagg tcagtgcatt taaaacataa agaaatgaag
     2341 agctagttca aaccttggga aaatacacta tatcttaaac tccatgaaag aaggtgaggc
     2401 tgcaaacagc taatgcacat tggcaacagc ccctgatgcc tatgccttat tcatccctca
     2461 gaaaaggatt caagtagagg cttgatttgg aggttaaagt tttgctatgc tgtattttac
     2521 attacttatt gttttagctg tcctcatgaa tgtcttttca ctacccattt gcttatcctg
     2581 catctctcag ccttgactcc actcagttct cttgcttaga gataccacct ttcccctgaa
     2641 gtgttccttc catgttttac ggcgagatgg tttctcctcg cctggccact cagccttagt
     2701 tgtctctgtt gtcttataga ggtctacttg aagaaggaaa aacagggggc atggtttgac
     2761 tgtcctgtga gcccttcttc cctgcctccc ccactcacag tgacccggaa tccctcgaca
     2821 tctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     2881 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     2941 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3001 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3061 gaatggcgaa tggcgattcc gttgcaatgg ctggcggtaa tattgttctg gatattacca
     3121 gcaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact aatcaaagaa
     3181 gtattgcgac aacggttaat ttgcgtgatg gacagactct tttactcggt ggcctcactg
     3241 attataaaaa cacttctcag gattctggcg taccgttcct gtctaaaatc cctttaatcg
     3301 gcctcctgtt tagctcccgc tctgattcta acgaggaaag cacgttatac gtgctcgtca
     3361 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta aatatttgct
     3841 tatacaatct tcctgttttt ggggcttttc tgattatcaa ccggggtaca tatgattgac
     3901 atgctagttt tacgattacc gttcatcgat tctcttgttt gctccagact ctcaggcaat
     3961 gacctgatag cctttgtaga gacctctcaa aaatagctac cctctccggc atgaatttat
     4021 cagctagaac ggttgaatat catattgatg gtgatttgac tgtctccggc ctttctcacc
     4081 cgtttgaatc tttacctaca cattactcag gcattgcatt taaaatatat gagggttcta
     4141 aaaattttta tccttgcgtt gaaataaagg cttctcccgc aaaagtatta cagggtcata
     4201 atgtttttgg tacaaccgat ttagctttat gctctgaggc tttattgctt aattttgcta
     4261 attctttgcc ttgcctgtat gatttattgg atgttggaat cgcctgatgc ggtattttct
     4321 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     4381 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4441 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4501 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4561 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4621 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4681 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4741 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4801 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4861 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4921 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4981 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5041 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5101 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5161 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5221 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5281 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5341 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5401 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5461 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5521 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5581 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5641 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5701 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5821 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5881 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5941 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6001 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6061 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6541 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc
     6601 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
     6661 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
     6721 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctagcggc
     6781 ctcggcctct gcataaataa aaaaaattag tcagccatga gcttggccca ttgcatacgt
     6841 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta c
//
