LOCUS       sequence-364699-       12356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the BASU RaPID labeling protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Ramanathan M, Majzoub K, Rao DS, Neela PH, Zarnegar BJ, Mondal S, 
            Roth JG, Gai H, Kovalski JR, Siprashvili Z, Palmer TD, Carette JE, 
            Khavari PA
  TITLE     RNA-protein interaction detection in living cells.
  JOURNAL   Nat Methods. 2018 Feb 5. pii: nmeth.4601. doi: 10.1038/nmeth.4601.
  PUBMED    29400715
REFERENCE   2  (bases 1 to 12356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Feb 5. pii: nmeth.4601. doi: 10.1038/nmeth.4601."
COMMENT     Sequence Label: BASU RaPID plasmid
FEATURES             Location/Qualifiers
     source          1..12356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    669..794
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1291..1524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1709..1753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2051..2168
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2233..2607)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2604..2613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2617..2635)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2627..2674)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2779..3082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3083..3286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3237..3257
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3283..3307
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3480..3506
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3516..3542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4346..4918
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4513..4530)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4740..4759
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4921..5520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5420..5440
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5530..5583
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5590..5599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5596..6306
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5741..5759)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6009..6028
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6319..6907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6372..6392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6790..6801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7114..7347
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7448..7465)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7454..7678
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7724..8152
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7811..7830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8021..8042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8161..8181)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8166..8495
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8346..8481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8408..8427
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8544..9569
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8544..9569
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    9699..9820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9869..9885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9869..9885)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9882..9904)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9893..9909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9917..9923,9924..9941,9942..9947))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9962..9983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10100..10117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10271..10859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10351..10370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11030..11821,11822..11890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11653..11672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11891..11995)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12043..12177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             join(12345..12356,1..622)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
       61 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
      121 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
      181 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
      241 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
      301 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
      361 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
      421 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      601 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
      661 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      721 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      781 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
      841 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
      901 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
      961 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1021 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1081 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     1141 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1201 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1261 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1321 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1381 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1441 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1501 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1561 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1621 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1681 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1741 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1801 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1861 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     1921 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     1981 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     2041 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     2101 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     2161 aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc
     2221 tctagtccgg aatcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc
     2281 gcgcagggcg aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga
     2341 ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc
     2401 gcgcacccac acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat
     2461 gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga
     2521 gaacccgagc cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac
     2581 cggaacggca ctggtcaact tggccatggt ggccctccta tagtgagtcg tattatacta
     2641 tgccgatata ctatgccgat gattaattgt caacacgtgc tgcaggtccg aggttctaga
     2701 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     2761 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2821 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2881 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2941 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3001 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3061 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3121 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3181 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     3241 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     3301 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     3361 ctagaggatc catgaacgca cgaacacgac gacgtgagcg tcgcgctgag aaacaagctc
     3421 aatggaaagc tgcaaactca ggaggcggtg ggtctggtgg cgggagcggc cgcctgcagc
     3481 tgccccccct ggagcgcctg accctggacc tcgagtaccc atacgatgtg cctgactatg
     3541 ctggcaagtt gtctgaatca gagattaggt ttggactgaa aacggaggtg atgggccaac
     3601 atcttatcta tcacgatgtt ttgtccagca cacaaaagac agcccatgaa ttggcaaata
     3661 acaatgcccc cgagggtacc ctcgtggttg cagataaaca gacagccggt aggggcggga
     3721 tgagccgagt gtggcattct caagaaggaa acggagtatg gatgagtttg atattgcgcc
     3781 cggacattcc gctccaaaag acgccccaac ttaccttgct ggcagctgta gcggtggttc
     3841 agggaataga agaggccgcg ggcatacaga ctgatataaa gtggccgaac gacatactca
     3901 ttaacgggaa aaaaacagta gggatactta ccgaaatgca agctgaggag gatcgagtgc
     3961 gctccgtgat tattggtatt ggcattaatg taaatcagca acccaatgat ttccccgatg
     4021 agctcaagga cattgcgacg agtctgtccc aggcagctgg ggaaaagatc gacagggcag
     4081 gtgtaatcca gcatatcttg ctttgtttcg aaaagcgata ccgagactac atgacccacg
     4141 gtttcacgcc catcaagctc ttgtgggagt catacgccct cggtattggc acgaacatga
     4201 gggcgagaac tctcaacggg acattttacg gtaaggcgtt gggtattgac gacgaagggg
     4261 tacttttgtt ggaaactaat gaaggcatta agaagatata cagcgcggac atcagcctta
     4321 ggtgaacgcg tctggccacc ggtcgcccct ctccctcccc cccccntaac gttactggcc
     4381 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4441 cgtcttttgg caatgtgtgg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4501 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4561 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4621 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4681 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     4741 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     4801 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     4861 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     4921 atggccaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
     4981 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
     5041 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5101 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5161 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5221 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5281 gagcccgcgt ggttcctggc caccgtcggc gtatcgcccg accaccaggg caagggtctg
     5341 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     5401 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     5461 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     5521 ggatcgggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     5581 ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     5641 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     5701 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     5761 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     5821 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     5881 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     5941 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     6001 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     6061 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     6121 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     6181 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     6241 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     6301 aagtaagaat tcgttaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6361 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6421 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6481 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6541 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6601 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6661 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6721 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6781 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6841 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6901 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     6961 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     7021 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7081 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     7141 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7201 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7261 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7321 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7381 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgt ttaaacccgc
     7441 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7501 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7561 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7621 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7681 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     7741 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7801 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7861 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7921 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7981 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8041 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8101 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     8161 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8221 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     8281 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     8341 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8401 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     8461 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     8521 ttttcggatc tgatcagcac gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga
     8581 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     8641 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     8701 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     8761 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     8821 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     8881 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     8941 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     9001 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     9061 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     9121 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     9181 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     9241 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     9301 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     9361 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     9421 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     9481 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     9541 ccagcactcg tccgagggca aaggaatagc acgtgctacg agatttcgat tccaccgccg
     9601 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     9661 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     9721 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9781 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     9841 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9901 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9961 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    10021 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10081 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10681 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    11041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    11101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    11161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    11221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    11281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11341 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11641 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    12001 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    12061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    12121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    12181 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    12241 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12301 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaa
//
