LOCUS       sequence-364703-        5906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Wang Z, Rong YP, Malone MH, Davis MC, Zhong F, Distelhorst CW
  TITLE     Thioredoxin-interacting protein (txnip) is a 
            glucocorticoid-regulated primary response gene involved in mediating
            glucocorticoid-induced apoptosis.
  JOURNAL   Oncogene. 2006 Mar 23. 25(13):1903-13.
  PUBMED    16301999
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2006 Mar 23. 25(13):1903-13."
COMMENT     Sequence Label: GFP-TXNIP
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        401..604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     555..575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(640..642,643..645,646..1356)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(685..706)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(946..965)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1293..1314
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2730..2851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2767..2786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2821..2840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2858..3313)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2995..3016)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3207..3226
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3340..3444
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3446..3803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3446..3637
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3469..3489)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3654..3789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3838..4632
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3838..4632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3892..3911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4502..4521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4820..4839)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4864..4911
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5240..5828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5729..5748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
       61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      601 agctggttta gtgaaccgtc agatccgcta gccgccacca tggtgagcaa gggcgaggag
      661 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      721 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      781 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac
      841 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      901 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      961 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1021 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1081 agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1141 acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1201 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1261 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1321 gccgggatca ctctcggcat ggacgagctg tacaagctca gatctcgagc tcaagcttcg
     1381 aattccgtga tgttcaagaa gatcaagtct tttgaggtgg tcttcaacga ccccgagaag
     1441 gtgtacggca gcggggagaa ggtggccgga cgggtaatag tggaagtgtg tgaagttacc
     1501 cgagtcaaag ccgtcaggat cctggcttgc ggcgtggcca aggtcctgtg gatgcaaggg
     1561 tctcagcagt gcaaacagac tttggactac ttgcgctatg aagacacact tctcctagaa
     1621 gagcagccta cagcaggtga gaacgagatg gtgatcatga ggcctggaaa caaatatgag
     1681 tacaagttcg gcttcgagct tcctcaaggg cccctgggaa catcctttaa aggaaaatat
     1741 ggttgcgtag actactgggt gaaggctttt ctcgatcgcc ccagccagcc aactcaagag
     1801 gcaaagaaaa acttcgaagt gatggatcta gtggatgtca atacccctga cttaatggca
     1861 ccagtgtctg ccaaaaagga gaagaaagtt tcctgcaagt tcattcctga tggacgtgtg
     1921 tcagtctctg ctcgaattga cagaaaagga ttctgtgaag gtgatgacat ctccatccat
     1981 gctgactttg agaacacgtg ttcccgaatc gtggtcccca aagcggctat tgtggcccga
     2041 cacacttacc ttgccaatgg ccagaccaaa gtgttcactc agaagctgtc ctcagtcaga
     2101 ggcaatcaca ttatctcagg gacttgcgca tcttggcgtg gcaagagcct cagagtgcag
     2161 aagatcagac catccatcct gggctgcaac atcctcaaag tcgaatactc cttgctgatc
     2221 tacgtcagtg tccctggctc caagaaagtc atccttgatc tgcccctagt gattggcagc
     2281 aggtctggtc tgagcagccg gacatccagc atggccagcc ggacgagctc tgagatgagc
     2341 tggatagacc taaacatccc agatacccca gaagctcctc cttgctatat ggacatcatt
     2401 cctgaagatc acagactaga gagccccacc acccctctgc tggatgatgt ggacgactct
     2461 caagacagcc ctatctttat gtacgcccct gagttccagt tcatgccccc acccacttac
     2521 actgaggtgg atccgtgcgt ccttaacaac aacaacaaca acaacgtgca gtgagcctgg
     2581 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
     2641 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     2701 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2821 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
     2881 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2941 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3001 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3061 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3121 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3181 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3241 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3301 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3361 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3421 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     3481 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3541 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3601 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3661 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3721 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3781 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     3841 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     3901 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     3961 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     4021 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4081 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4141 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4201 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4261 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4321 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     4381 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4441 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4501 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4561 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4621 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     4681 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     4741 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     4801 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     4861 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     4921 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     4981 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5041 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     5101 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5881 ctcacatgtt ctttcctgcg ttatcc
//
