LOCUS       sequence-364709-       12546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enCas9 fused to PolI5M in E. coli. contains a 
            BsmbI-flanked GFP cassette for sgRNA cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE
  TITLE     CRISPR-guided DNA polymerases enable diversification of all 
            nucleotides in a tunable window.
  JOURNAL   Nature. 2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 
            10.1038/s41586-018-0384-8.
  PUBMED    30069054
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 
            10.1038/s41586-018-0384-8."
COMMENT     Sequence Label: pEvolvR-enCas9-PolI5M
FEATURES             Location/Qualifiers
     source          1..12546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..24
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        116..171
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             189..200
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             5093..5101
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(7303..8118)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7303..8118)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8026..8045
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8110..8129
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8347..8935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8836..8855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9089..9106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9121..9260
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9186..9205)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9346..9368
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9362..9553)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        9642..9676
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             9683..9694
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             9701..10360
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKF*PAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9767..9786)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        10470..10504
                     /label=BBa_J23115
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.15"
     RBS             10660..10671
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             10678..11391
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      11403..11474
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11490..11517
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        11531..11606
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             11627..11656
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             11672..11689
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11745..11762)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      11915..11961
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(12020..12546,1..100))
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
ORIGIN
        1 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
       61 cgctgttaat cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga
      121 cactctatcg ttgatagagt tattttacca ctccctatca gtgatagaga aaagaattca
      181 aaagatctaa agaggagaaa agatctatgg acaagaagta ttctatcgga ctggctatcg
      241 ggactaatag cgtcgggtgg gccgtgatca ctgacgagta caaggtgccc tctaagaagt
      301 tcaaggtgct cgggaacacc gaccggcatt ccatcaagaa aaatctgatc ggagctctcc
      361 tctttgattc aggggaaacc gctgaagcaa cccgcctcaa gcggactgct agacggcggt
      421 acaccaggag gaagaaccgg atttgttacc ttcaagagat attctccaac gaaatggcaa
      481 aggtcgacga cagcttcttc cataggctgg aagaatcatt cctcgtggaa gaggataaga
      541 agcatgaacg gcatcccatc ttcggtaata tcgtcgacga ggtggcctat cacgagaaat
      601 acccaaccat ctaccatctt cgcaaaaagc tggtggactc aaccgacaag gcagacctcc
      661 ggcttatcta cctggccctg gcccacatga tcaagttcag aggccacttc ctgatcgagg
      721 gcgacctcaa tcctgacaat agcgatgtgg ataaactgtt catccagctg gtgcagactt
      781 acaaccagct ctttgaagag aaccccatca atgcaagcgg agtcgatgcc aaggccattc
      841 tgtcagcccg gctgtcaaag agccgcagac ttgagaatct tatcgctcag ctgccgggtg
      901 aaaagaaaaa tggactgttc gggaacctga ttgctctttc acttgggctg actcccaatt
      961 tcaagtctaa tttcgacctg gcagaggatg ccaagctgca actgtccaag gacacctatg
     1021 atgacgatct cgacaacctc ctggcccaga tcggtgacca atacgccgac cttttccttg
     1081 ctgctaagaa tctttctgac gccatcctgc tgtctgacat tctccgcgtg aacactgaaa
     1141 tcaccaaggc ccctctttca gcttcaatga ttaagcggta tgatgagcac caccaggacc
     1201 tgaccctgct taaggcactc gtccggcagc agcttccgga gaagtacaag gaaatcttct
     1261 ttgaccagtc aaagaatgga tacgccggct acatcgacgg aggtgcctcc caagaggaat
     1321 tttataagtt tatcaaacct atccttgaga agatggacgg caccgaagag ctcctcgtga
     1381 aactgaatcg ggaggatctg ctgcggaagc agcgcacttt cgacaatggg agcattcccc
     1441 accagatcca tcttggggag cttcacgcca tccttcggcg ccaagaggac ttctacccct
     1501 ttcttaagga caacagggag aagattgaga aaattctcac tttccgcatc ccctactacg
     1561 tgggacccct cgccagagga aatagccggt ttgcttggat gaccagaaag tcagaagaaa
     1621 ctatcactcc ctggaacttc gaagaggtgg tggacaaggg agccagcgct cagtcattca
     1681 tcgaacggat gactaacttc gataagaacc tccccaatga gaaggtcctg ccgaaacatt
     1741 ccctgctcta cgagtacttt accgtgtaca acgagctgac caaggtgaaa tatgtcaccg
     1801 aagggatgag gaagcccgca ttcctgtcag gcgaacaaaa gaaggcaatt gtggaccttc
     1861 tgttcaagac caatagaaag gtgaccgtga agcagctgaa ggaggactat ttcaagaaaa
     1921 ttgaatgctt cgactctgtg gagattagcg gggtcgaaga tcggttcaac gcaagcctgg
     1981 gtacttacca tgatctgctt aagatcatca aggacaagga ttttctggac aatgaggaga
     2041 acgaggacat ccttgaggac attgtcctga ctctcactct gttcgaggac cgggaaatga
     2101 tcgaggagag gcttaagacc tacgcccatc tgttcgacga taaagtgatg aagcaactta
     2161 aacggagaag atataccgga tggggacgcc ttagccgcaa actcatcaac ggaatccggg
     2221 acaaacagag cggaaagacc attcttgatt tccttaagag cgacggattc gctaatcgca
     2281 acttcatgca acttatccat gatgattccc tgacctttaa ggaggacatc cagaaggccc
     2341 aagtgtctgg acaaggtgac tcactgcacg agcatatcgc aaatctggct ggttcacccg
     2401 ctattaagaa gggtattctc cagaccgtga aagtcgtgga cgagctggtc aaggtgatgg
     2461 gtcgccataa accagagaac attgtcatcg agatggccag ggaaaaccag actacccaga
     2521 agggacagaa gaacagcagg gagcggatga aaagaattga ggaagggatt aaggagctcg
     2581 ggtcacagat ccttaaagag cacccggtgg aaaacaccca gcttcagaat gagaagctct
     2641 atctgtacta ccttcaaaat ggacgcgata tgtatgtgga ccaagagctt gatatcaaca
     2701 ggctctcaga ctacgacgtg gaccatatcg tccctcagag cttcctcgca gacgactcaa
     2761 ttgacaataa ggtgctgact cgctcagaca agaaccgggg aaagtcagat aacgtgccct
     2821 cagaggaagt cgtgaaaaag atgaagaact attggcgcca gcttctgaac gcaaagctga
     2881 tcactcagcg gaagttcgac aatctcacta aggctgagag gggcggactg agcgaactgg
     2941 acaaagcagg attcattaaa cggcaacttg tggagactcg gcagattact aaacatgtcg
     3001 cccaaatcct tgactcacgc atgaatacca agtacgacga aaacgacaaa cttatccgcg
     3061 aggtgaaggt gattaccctg aagtccaagc tggtcagcga tttcagaaag gactttcaat
     3121 tctacaaagt gcgggagatc aataactatc atcatgctca tgacgcatat ctgaatgccg
     3181 tggtgggaac cgccctgatc aagaagtacc cagcactgga aagcgagttc gtgtacggag
     3241 actacaaggt ctacgacgtg cgcaagatga ttgccaaatc tgagcaggag atcggaaagg
     3301 ccaccgcaaa gtacttcttc tacagcaaca tcatgaattt cttcaagacc gaaatcaccc
     3361 ttgcaaacgg tgagatccgg aaggcgccgc tcatcgagac taatggggag actggcgaaa
     3421 tcgtgtggga caagggcaga gatttcgcta ccgtgcgcaa agtgctttct atgcctcaag
     3481 tgaacatcgt gaagaaaacc gaggtgcaaa ccggaggctt ttctaaggaa tcaatcctcc
     3541 ccaagcgcaa ctccgacaag ctcattgcaa ggaagaagga ttgggaccct aagaagtacg
     3601 gcggattcga ttcaccaact gtggcttatt ctgtcctggt cgtggctaag gtggaaaaag
     3661 gaaagtctaa gaagctcaag agcgtgaagg aactgctggg tatcaccatt atggagcgca
     3721 gctccttcga gaagaaccca attgactttc tcgaagccaa aggttacaag gaagtcaaga
     3781 aggaccttat catcaagctc ccaaagtata gcctgttcga actggagaat gggcggaagc
     3841 ggatgctcgc ctccgctggc gaacttcaga agggtaatga gctggctctc ccctccaagt
     3901 acgtgaattt cctctacctt gcaagccatt acgagaagct gaaggggagc cccgaggaca
     3961 acgagcaaaa gcaactgttt gtggagcagc ataagcatta tctggacgag atcattgagc
     4021 agatttccga gttttctaaa cgcgtcattc tcgctgatgc caacctcgat aaagtcctta
     4081 gcgcatacaa taagcacaga gacaaaccaa ttcgggagca ggctgagaat atcatccacc
     4141 tgttcaccct caccaatctt ggtgcccctg ccgcattcaa gtacttcgac accaccatcg
     4201 accggaaacg ctatacctcc accaaagaag tgctggacgc caccctcatc caccagagca
     4261 tcaccggact ttacgaaact cggattgacc tctcacagct cggaggggat ggttctagtg
     4321 aaaccccggg aacaagtgag tcggccaccc ctgaaggtgg atcagggggt agcggatccg
     4381 ttcagatccc gcagaacccg ctgattctgg ttgacggatc tagttacctg taccgtgctt
     4441 accatgcttt cccgcctttg accaattctg ctggtgaacc tacgggagct atgtacggag
     4501 ttctgaatat gttgcgttct ttaattatgc agtacaagcc tacccacgct gctgttgttt
     4561 tcgatgctaa aggtaagacg ttccgcgacg agttattcga gcactataag tctcaccgtc
     4621 ctccgatgcc tgatgactta cgcgctcaga ttgagccgct gcatgctatg gtgaaggcta
     4681 tgggtttacc tcttttggct gtcagcggtg ttgaggctga tgatgtcatt ggcaccttag
     4741 ctcgtgaggc tgagaaggct ggtcgccctg ttttgatttc taccggtgac aaggacatgg
     4801 ctcaattggt taccccgaac atcaccctga tcaacaccat gaccaacacg attctgggtc
     4861 ctgaggaagt tgttaacaaa tatggtgttc ctccggagtt gattattgac tttcttgctc
     4921 ttatgggcga ttcttcagac aatatcccgg gtgttccagg tgttggagag aagactgctc
     4981 aagctctgct tcagggtctg ggtggtttgg acacccttta cgctgaaccg gagaagatcg
     5041 ccggtctgtc ttttcgcggt gctaagacca tggctgctaa actggaacag aataaggagg
     5101 tcgcatacct gtcttatcaa ttggctacca tcaagacgga tgtggagtta gaacttacgt
     5161 gcgagcagct tgaggttcaa cagcctgctg ctgaggaact gctgggtctt tttaagaaat
     5221 acgaatttaa gcgttggacc gccgacgttg aggctggtaa gtggctgcaa gctaagggtg
     5281 ctaagccggc tgctaaaccg caagaaacga gtgtcgctga tgaggctccg gaggttaccg
     5341 ctaccgttat ctcttacgat aattatgtta cgattctgga cgaggaaacc ttaaaggctt
     5401 ggatcgctaa attagagaag gctcctgttt tcgctttcga cacggaaacg gattctctgg
     5461 acaatattag tgcgaatctt gttggtctga gtttcgcaat tgaaccgggt gttgctgctt
     5521 acatccctgt ggcacacgac tacctggacg ctccggacca gatttcacgt gaacgcgctc
     5581 tggaactact gaagccttta ttagaggacg agaaagcttt gaaagttggt cagaatttga
     5641 agtatgctcg tggaatctta gctaattatg gtatcgagtt gcgcggtatc gctttcgaca
     5701 cgatgttgga atcttatatc ctgaactctg tcgctggtcg ccatgacatg gactctctgg
     5761 ctgagcgctg gctgaaacat aagacgatta ccttcgagga aatcgcagga aagggtaaga
     5821 accagctcac gttcaatcaa atcgctctgg aggaagctgg tcgctatgct gctgaggacg
     5881 ctgacgttac tctgcaactg cacttgaaga tgtggcctga cttgcagaag cataagggtc
     5941 cactgaatgt ttttgaaaac attgagatgc ctttggttcc agttctgtct cgtatcgagc
     6001 gcaatggcgt taaaattgac ccaaaggttt tacataacca ctcagaggaa ctgacgctgc
     6061 gcttagccga attggagaaa aaggctcacg agatcgctgg cgaagagttc aatctgtcat
     6121 ctacgaaaca actgcagact atcctgttcg agaagcaagg tatcaagcca ttaaaaaaga
     6181 cccctggcgg tgctccgtct acctctgagg aagttttgga ggagttagct ttggattacc
     6241 ctctgccgaa ggttatcttg gaataccgcg gtttggctaa attgaagtct acttatacgg
     6301 ataaacttcc tttgatgatt aatccaaaga cgggtcgcgt tcacacgtcg taccatcaag
     6361 ctgttaccgc taccggtcgc ctgtcttcta cggatccgaa tttacagaat attcctgtgc
     6421 gcaatgagga gggccgccgc attcgtcaag cttttatcgc tccggaagac tacgttatcg
     6481 tttctgctga ttattctcaa aatgaattac gtatcatggc tcacctgtct cgcgataagg
     6541 gtctgttgac ggcctttgct gagggtaagg acattcatcg tgctaccgct gctgaggttt
     6601 acggcctgcc gttggaaacg gttacgtctg aacagcgtcg ctctgctaag cgtattaatt
     6661 tcggcttaat ctacggtatg tctgcgtttg gcttagctcg tcagctgaat atcccgcgca
     6721 aggaagctca aaaatatatg gatctgtatt ttgagcgtta ccacggtgtt ttggaataca
     6781 tggagcgtac gcgcgcgcaa gctaaggaac aaggttatgt ggaaaccttg gatggtcgtc
     6841 gcttgtactt gcctgacatt aagtcttcta acggcgcccg ccgcgctgct gccgagcgcg
     6901 ctgctatcaa tgctccgatg caaggtactg ctgctgatat tattaagcgt gctatgatcg
     6961 ctgtggacgc ttggctgcaa gctgaacagc ctcgcgttcg catgattatg caagttcatg
     7021 acgagttggt tttcgaggtg cataaggacg acgtggacgc tgttgctaaa caaatccacc
     7081 agttgatgga gaattgcacg cgcttagacg ttccgctgct ggttgaagtt ggttctggtg
     7141 aaaactggga ccaggctcac taataatggc ctcggtacca aagacgaaca ataagacgct
     7201 gaaaagcgtc ttttttcgtt ttggtccgct gagcagttac agagatgtta cgaaccacta
     7261 gtgcactgca gtacagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag
     7321 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     7381 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     7441 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     7501 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     7561 caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     7621 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa
     7681 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     7741 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     7801 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     7861 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     7921 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     7981 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     8041 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc
     8101 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     8161 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac
     8221 gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcaggagt tcatgaccaa
     8281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8461 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8761 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8941 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9001 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9061 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9121 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
     9181 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     9241 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9301 cttgtctgct cccggcatcc gcttacagac aagctgtgac cttctccggg agctgcatgt
     9361 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     9421 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     9481 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     9541 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatccct gaattcgcat
     9601 ctagactgaa ctggccgata attgcagacg aacggattta ctttacagct agctcagtcc
     9661 taggtattat gctagcgcta gcaaagagga gaaaagatct atggagaaaa aaatcactgg
     9721 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     9781 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     9841 cgtaaagaaa aataagcaca agttttaacc ggcctttatt cacattcttg cccgcctgat
     9901 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     9961 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
    10021 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
    10081 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtttcagc
    10141 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
    10201 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
    10261 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
    10321 attacaacag tactgcgatg agtggcaggg cggggcgtaa ggatcctaac tcgagctcgg
    10381 taccaaattc cagaaaagag gcctcccgaa aggggggcct tttttcgttt tggtccgctg
    10441 ccaaaccaga tgtcaacaca gctacaacgt ttatagctag ctcagccctt ggtacaatgc
    10501 tagctgagac ggaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt
    10561 atttaataat gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc
    10621 aactcaagaa acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg
    10681 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
    10741 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
    10801 ctgacgctga agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta
    10861 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
    10921 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
    10981 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
    11041 cgcattgagc tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg
    11101 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt
    11161 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
    11221 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
    11281 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
    11341 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc
    11401 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
    11461 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
    11521 atacgtctca gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
    11581 ttgaaaaagt ggcaccgagt cggtgctttt tttgaagctt gggcccgaac aaaaactcat
    11641 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac
    11701 ggactccagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
    11761 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgaggtggtc
    11821 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
    11881 actccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
    11941 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gacagggctg gatgcacaca
    12001 ctggcttaag atgacaacgt taagacccac tttcacattt aagttgtttt tctaatccgc
    12061 atatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt
    12121 cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc ctttcttctt
    12181 tagcgacttg atgctcttga tcttccaata cgcaacctaa agtaaaatgc cccacagcgc
    12241 tgagtgcata taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat
    12301 tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat
    12361 cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc
    12421 agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta
    12481 aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac
    12541 ctagct
//
