LOCUS       sequence-36471-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP Asn10 TEV LIC cloning vector (1C)
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atataccatg ggttcttctc accatcacca tcaccatggt
      181 tcttctatga aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat
      241 aacggtctcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt
      301 gagcatccgg ataaactgga agagaaattc ccacaggttg cggcaactgg cgatggccct
      361 gacattatct tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct
      421 gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacctg ggatgccgta
      481 cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc gctgatttat
      541 aacaaagatc tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa
      601 gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc
      661 tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac
      721 attaaagacg tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac
      781 ctgattaaaa acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt
      841 aataaaggcg aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc
      901 agcaaagtga attatggtgt aacggtactg ccgaccttca agggtcaacc atccaaaccg
      961 tt
//
