LOCUS       sequence-364717-        8423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Johnson BS, McCaffery JM, Lindquist S, Gitler AD
  TITLE     A yeast TDP-43 proteinopathy model: Exploring the molecular 
            determinants of TDP-43 aggregation and cellular toxicity.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Apr 29. 105(17):6439-44.
  PUBMED    18434538
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Apr 29. 105(17):6439-44."
COMMENT     Sequence Label: pRS426 Gal TDP43 GFP
FEATURES             Location/Qualifiers
     source          1..8423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        178..619
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    178..295
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     590..613
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     634..650
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     634..650
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    658..682
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1943..1967)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1999..2001,2002..2004,2005..2718)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1999..2001,2002..2004,2005..2715)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2044..2065)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2305..2324)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2652..2673
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2725..2972
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2741..2759)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(2990..3009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2991..3009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3019..3036)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3019..3035)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3028..3050)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3176..3631
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3263..3282)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3473..3494
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3762..4565)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4566..4781)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4805..4824)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4924..4946
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4984..5002)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      5043..6385
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    5375..5422
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6412..6516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6517..6585,6586..7377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6735..6754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7548..8136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8037..8056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8290..8307
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tctagtacgg
      181 attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc
      241 gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa
      301 taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg
      361 ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag
      421 ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac
      481 agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt
      541 tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct
      601 atactttaac gtcaaggaga aaaaaccccg gattctagaa ctagtggatc ccccatcaca
      661 agtttgtaca aaaaagcagg cttcgaagga gataacaaaa tgtctgaata tattcgggta
      721 accgaagatg agaacgatga gcccattgaa ataccatcgg aagacgatgg gacggtgctg
      781 ctctccacgg ttacagccca gtttccaggg gcgtgtgggc ttcgctacag gaatccagtg
      841 tctcagtgta tgagaggtgt ccggctggta gaaggaattc tgcatgcccc agatgctggc
      901 tggggaaatc tggtgtatgt tgtcaactat ccaaaagata acaaaagaaa aatggatgag
      961 acagatgctt catcagcagt gaaagtgaaa agagcagtcc agaaaacatc cgatttaata
     1021 gtgttgggtc tcccatggaa aacaaccgaa caggacctga aagagtattt tagtaccttt
     1081 ggagaagttc ttatggtgca ggtcaagaaa gatcttaaga ctggtcattc aaaggggttt
     1141 ggctttgttc gttttacgga atatgaaaca caagtgaaag taatgtcaca gcgacatatg
     1201 atagatggac gatggtgtga ctgcaaactt cctaattcta agcaaagcca agatgagcct
     1261 ttgagaagca gaaaagtgtt tgtggggcgc tgtacagagg acatgactga ggatgagctg
     1321 cgggagttct tctctcagta cggggatgtg atggatgtct tcatccccaa gccattcagg
     1381 gcctttgcct ttgttacatt tgcagatgat cagattgcgc agtctctttg tggagaggac
     1441 ttgatcatta aaggaatcag cgttcatata tccaatgccg aacctaagca caatagcaat
     1501 agacagttag aaagaagtgg aagatttggt ggtaatccag gtggctttgg gaatcagggt
     1561 ggatttggta atagcagagg gggtggagct ggtttgggaa acaatcaagg tagtaatatg
     1621 ggtggtggga tgaactttgg tgcgttcagc attaatccag ccatgatggc tgccgcccag
     1681 gcagcactac agagcagttg gggtatgatg ggcatgttag ccagccagca gaaccagtca
     1741 ggcccatcgg gtaataacca aaaccaaggc aacatgcaga gggagccaaa ccaggccttc
     1801 ggttctggaa ataactctta tagtggctct aattctggtg cagcaattgg ttggggatca
     1861 gcatccaatg cagggtcggg cagtggtttt aatggaggct ttggctcaag catggattct
     1921 aagtcttctg gctggggaat ggacccagct ttcttgtaca aagtggtgat gggctgcagg
     1981 aattcgatat caagcttaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2161 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2221 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2461 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2581 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     2641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2701 gacgagctgt acaagtaact cgagtcatgt aattagttat gtcacgctta cattcacgcc
     2761 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
     2821 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
     2881 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     2941 aggttttggg acgctcgaag gctttaattt gcggccggta cccaattcgc cctatagtga
     3001 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3061 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     3181 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3241 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3301 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3361 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3421 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3481 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3541 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3601 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     3661 tgtgcggtat ttcacaccgc atagggtaat aactgatata attaaattga agctctaatt
     3721 tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg ctggccgcat
     3781 cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac cttagcatcc
     3841 cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt agagaccaca
     3901 tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa acccacaccg
     3961 ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata
     4021 aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt agtatattct
     4081 ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc
     4141 tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg
     4201 tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga gtactgcaat
     4261 ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa attgtacttg
     4321 gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt caagatatcc
     4381 acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc cagtaattcc
     4441 ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg catgatatta
     4501 aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata tgtagctttc
     4561 gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt taagaatact
     4621 gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct aagtctgtgc
     4681 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac
     4741 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta tggtgcactc
     4801 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4861 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4921 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4981 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagt
     5041 atgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt
     5101 ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga
     5161 atgggataat atcacaggag gtactagact acctttcatc ctacataaat agacgcatat
     5221 aagtacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca
     5281 ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg
     5341 cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt
     5401 ctctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca
     5461 ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg
     5521 cttccacaaa cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat
     5581 ataacctacc catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt
     5641 tttatgttta tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata
     5701 gagtgaatcg aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa
     5761 aatagaagaa accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc
     5821 tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct
     5881 tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct
     5941 ttttttatgg aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac
     6001 agtgcaaaaa gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc
     6061 taagatgctt tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa
     6121 gtatagattc tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg
     6181 cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa
     6241 atgaagcaca gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa
     6301 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta
     6361 caaaatgaag cacagatgct tcgttcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6721 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7021 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7081 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7141 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7201 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7261 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7321 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7381 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7681 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8221 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8281 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8401 gcgggcagtg agcgcaacgc aat
//
