LOCUS       sequence-36473-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 NusA TEV LIC cloning vector (1N)
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggttcttc tcaccatcac catcaccatg gttcttctat
      181 gaacaaagaa attttggctg tagttgaagc cgtatccaat gaaaaggcgc tacctcgcga
      241 gaagattttc gaagcattgg aaagcgcgct ggcgacagca acaaagaaaa aatatgaaca
      301 agagatcgac gtccgcgtac agatcgatcg caaaagcggt gattttgaca ctttccgtcg
      361 ctggttagtt gttgatgaag tcacccagcc gaccaaggaa atcacccttg aagccgcacg
      421 ttatgaagat gaaagcctga acctgggcga ttacgttgaa gatcagattg agtctgttac
      481 ctttgaccgt atcactaccc agacggcaaa acaggttatc gtgcagaaag tgcgtgaagc
      541 cgaacgtgcg atggtggttg atcagttccg tgaacacgaa ggtgaaatca tcaccggcgt
      601 ggtgaaaaaa gtaaaccgcg acaacatctc tctggatctg ggcaacaacg ctgaagccgt
      661 gatcctgcgc gaagatatgc tgccgcgtga aaacttccgc cctggcgacc gcgttcgtgg
      721 cgtgctctat tccgttcgcc cggaagcgcg tggcgcgcaa ctgttcgtca ctcgttccaa
      781 gccggaaatg ctgatcgaac tgttccgtat tgaagtgcca gaaatcggcg aagaagtgat
      841 tgaaattaaa gcagcggctc gcgatccggg ttctcgtgcg aaaatcgcgg tgaaaaccaa
      901 cgataaacgt atcgatccgg taggtgcttg cgtaggtatg cgtggcgcgc gtgttcaggc
      961 ggtgtctact ga
//
