LOCUS       sequence-364739-         264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express phage alpha3 lysis protein E in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Ma Y, Liu Q, Hu A, Jiang S, Wang S, Liu R, Han K, Wang J
  TITLE     Construction and In Vitro Evaluation of a Tumor Acidic pH-Targeting 
            Drug Delivery System Based on Escherichia coli Nissle 1917 Bacterial
            Ghosts.
  JOURNAL   Bioengineering (Basel). 2022 Sep 2;9(9):433. doi: 
            10.3390/bioengineering9090433.
  PUBMED    36134979
REFERENCE   2  (bases 1 to 264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Bioengineering (Basel)."; date: "2022-09-2"; volume: "9(9):433. 
            doi"; pages: " 10.3390/bioengineering9090433"
COMMENT     Sequence Label: pET29a-alpha-3-E
FEATURES             Location/Qualifiers
     source          1..264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             238..261
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atggaacgct ggaccttact ggatattctg gcgtttctgc ttctgcttag cctgcttctg
       61 ccgagcctgc tgattatgtt tattccgagc atgtataaac agcatgcgag cctgtggaaa
      121 gcgcgcagcc tggcgaaaac cctgagcatg gcgagcagcg cgcgcctgac cccgctgagc
      181 agctctcgca ccccgtgcgt gctgaaacag gatagcaaaa aactgggtac tggcggttgg
      241 tctcatccgc agtttgaaaa ataa
//
