LOCUS       sequence-36474-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, 
            Gumper I, Sabatini DD, De Robertis EM
  TITLE     Wnt signaling requires sequestration of glycogen synthase kinase 3 
            inside multivesicular endosomes.
  JOURNAL   Cell. 2010 Dec 23. 143(7):1136-48.
  PUBMED    21183076
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Dec 23. 143(7):1136-48."
COMMENT     Sequence Label: DsRed-Rab5-QL
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..84
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    733..785
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 cagtacgagc gcaccgaggg ccgccaccac ctgttcctga gatctgccgc catggcatat
       61 ccatatgacg tcccagacta tgctggcgct agtcgaggcg caacaagacc caacgggcca
      121 aatactggaa ataaaatatg ccagttcaaa ctagtacttc tgggagagtc tgctgttggc
      181 aaatcaagcc tagtgcttcg ttttgtgaag ggccaatttc atgaatttca agagagtacc
      241 ataggggctg cttttctaac ccaaactgtg tgtcttgatg atacaacagt aaagtttgaa
      301 atatgggata cagctggtct agaacgatac catagcttag caccaatgta ctacagagga
      361 gcacaagcag ccatagttgt atatgatatc acaaatgagg agtcctttgc cagagccaaa
      421 aactgggtta aagaacttca gaggcaagcc agtcctaaca ttgtaatagc tttatcagga
      481 aacaaggctg atcttgcaaa taaaagagct gtcgatttcc aggaagcaca gtcctatgca
      541 gatgacaaca gtttattatt catggagaca tcagctaaaa catcgatgaa tgtaaatgaa
      601 atattcatgg caatagctaa aaagttgcca aagaacgaac cacagaatcc aggagcaaat
      661 tctgccagag gaagaggagt agaccttact gaacccacgc agccaaccag gaatcagtgt
      721 tgtagtaact agctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      781 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
      841 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      901 ttgt
//
