LOCUS       sequence-364759-        9817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, 
            expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses 
            Blasticidin resistance under PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Noviello G, Gjaltema RAF, Schulz EG
  TITLE     CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning 
            of endogenous gene expression.
  JOURNAL   Nat Commun. 2023 Jun 3;14(1):3225. doi: 10.1038/s41467-023-38909-4.
  PUBMED    37270532
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-3"; volume: "14(1):3225. doi"; pages: " 
            10.1038/s41467-023-38909-4"
COMMENT     Sequence Label: pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin
FEATURES             Location/Qualifiers
     source          1..9817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3..704
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    719..943
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1294..1313
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1369..1767
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    1768..2356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1821..1841)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2239..2250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   2539..2601
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2631..2651)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2631..2649)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2670..2686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2670..2686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2683..2705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2694..2710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2718..2724,2725..2742,2743..2748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2763..2784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2901..2918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3072..3660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3152..3171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3831..4622,4623..4691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4454..4473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4692..4796)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4822..5277)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4959..4980)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5171..5190
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5404..5426
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5418..5435
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5419..5435
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5445..5464
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5445..5463
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   5479..5513
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5835..6046
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6059..6327
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6379..6399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6409..6729
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6781..6801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6811..9708
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             9718..9738
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9748..9774
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
       61 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      121 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      181 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
      241 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      301 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      361 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
      421 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
      481 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
      541 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
      601 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
      661 gatactgcga cctccctagc aaactggggc acaagcttaa ttagccatgg attaacgact
      721 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      781 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      841 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
      901 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcctctgg cctcgcacac
      961 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     1021 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     1081 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     1141 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     1201 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     1261 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     1321 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcaat ggccaagcct
     1381 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     1441 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     1501 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     1561 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     1621 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     1681 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     1741 ccctctggtt atgtgtggga gggctaaaat caacctctgg attacaaaat ttgtgaaaga
     1801 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1861 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1921 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1981 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2041 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2101 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2161 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2221 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2281 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt
     2341 tgggccgcct ccccgccggt acctaaaagt tttgttactt tatagaagaa attttgagtt
     2401 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
     2461 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
     2521 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac
     2581 aatatgatta tctttctagg gttaatctag tattaatcca gcttttgttc cctttagtga
     2641 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2701 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2761 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2821 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2881 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3481 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3661 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3721 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3781 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3841 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3901 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3961 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4021 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4081 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4141 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4201 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4261 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4321 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4381 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4441 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4501 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4561 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4621 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4681 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4741 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4801 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4861 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4921 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4981 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     5041 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     5101 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     5161 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     5221 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     5281 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     5341 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     5401 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     5461 agggcgaatt ggggttaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt
     5521 gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc
     5581 agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt
     5641 gaactataac gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag
     5701 atttaactca tacgataatt atattgttat ttcatgttct acttacgtga taacttatta
     5761 tatatatatt ttcttgttat agatatcaac tagaatacgc gtaaggatct gcgatcgctc
     5821 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5881 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5941 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6001 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     6061 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     6121 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     6181 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     6241 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     6301 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctactc tagagctagc
     6361 gaattcgccg ccaccatgcc taagaaaaag aggaaggtga gcggccgcgg agttcaggtg
     6421 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
     6481 cactacaccg ggatgcttga agatggaaag aaagttgatt cctcccggga cagaaacaag
     6541 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
     6601 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
     6661 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
     6721 aaactggaag gcggatctgg aggtagtggt ggatctggag gttctggtgg ctccatgagc
     6781 cccaagaaga agagaaaggt ggaggccagc atcgaaaaaa aaaagtcctt cgccaagggc
     6841 atgggcgtga agtccacact cgtgtccggc tccaaagtgt acatgacaac cttcgccgaa
     6901 ggcagcgacg ccaggctgga aaagatcgtg gagggcgaca gcatcaggag cgtgaatgag
     6961 ggcgaggcct tcagcgctga aatggccgat aaaaacgccg gctataagat cggcaacgcc
     7021 aaattcagcc atcctaaggg ctacgccgtg gtggctaaca accctctgta tacaggaccc
     7081 gtccagcagg atatgctcgg cctgaaggaa actctggaaa agaggtactt cggcgagagc
     7141 gctgatggca atgacaatat ttgtatccag gtgatccata acatcctgga cattgaaaaa
     7201 atcctcgccg aatacattac caacgccgcc tacgccgtca acaatatctc cggcctggat
     7261 aaggacatta ttggattcgg caagttctcc acagtgtata cctacgacga attcaaagac
     7321 cccgagcacc atagggccgc tttcaacaat aacgataagc tcatcaacgc catcaaggcc
     7381 cagtatgacg agttcgacaa cttcctcgat aaccccagac tcggctattt cggccaggcc
     7441 tttttcagca aggagggcag aaattacatc atcaattacg gcaacgaatg ctatgacatt
     7501 ctggccctcc tgagcggact gaggcactgg gtggtccata acaacgaaga agagtccagg
     7561 atctccagga cctggctcta caacctcgat aagaacctcg acaacgaata catctccacc
     7621 ctcaactacc tctacgacag gatcaccaat gagctgacca actccttctc caagaactcc
     7681 gccgccaacg tgaactatat tgccgaaact ctgggaatca accctgccga attcgccgaa
     7741 caatatttca gattcagcat tatgaaagag cagaaaaacc tcggattcaa tatcaccaag
     7801 ctcagggaag tgatgctgga caggaaggat atgtccgaga tcaggaaaaa tcataaggtg
     7861 ttcgactcca tcaggaccaa ggtctacacc atgatggact ttgtgattta taggtattac
     7921 atcgaagagg atgccaaggt ggctgccgcc aataagtccc tccccgataa tgagaagtcc
     7981 ctgagcgaga aggatatctt tgtgattaac ctgaggggct ccttcaacga cgaccagaag
     8041 gatgccctct actacgatga agctaataga atttggagaa agctcgaaaa tatcatgcac
     8101 aacatcaagg aatttagggg aaacaagaca agagagtata agaagaagga cgcccctaga
     8161 ctgcccagaa tcctgcccgc tggccgtgat gtttccgcct tcagcaaact catgtatgcc
     8221 ctgaccatgt tcctggatgg caaggagatc aacgacctcc tgaccaccct gattaataaa
     8281 ttcgataaca tccagagctt cctgaaggtg atgcctctca tcggagtcaa cgctaagttc
     8341 gtggaggaat acgccttttt caaagactcc gccaagatcg ccgatgagct gaggctgatc
     8401 aagtccttcg ctagaatggg agaacctatt gccgatgcca ggagggccat gtatatcgac
     8461 gccatccgta ttttaggaac caacctgtcc tatgatgagc tcaaggccct cgccgacacc
     8521 ttttccctgg acgagaacgg aaacaagctc aagaaaggca agcacggcat gagaaatttc
     8581 attattaata acgtgatcag caataaaagg ttccactacc tgatcagata cggtgatcct
     8641 gcccacctcc atgagatcgc caaaaacgag gccgtggtga agttcgtgct cggcaggatc
     8701 gctgacatcc agaaaaaaca gggccagaac ggcaagaacc agatcgacag gtactacgaa
     8761 acttgtatcg gaaaggataa gggcaagagc gtgagcgaaa aggtggacgc tctcacaaag
     8821 atcatcaccg gaatgaacta cgaccaattc gacaagaaaa ggagcgtcat tgaggacacc
     8881 ggcagggaaa acgccgagag ggagaagttt aaaaagatca tcagcctgta cctcaccgtg
     8941 atctaccaca tcctcaagaa tattgtcaat atcaacgcca ggtacgtcat cggattccat
     9001 tgcgtcgagc gtgatgctca actgtacaag gagaaaggct acgacatcaa tctcaagaaa
     9061 ctggaagaga agggattcag ctccgtcacc aagctctgcg ctggcattga tgaaactgcc
     9121 cccgataaga gaaaggacgt ggaaaaggag atggctgaaa gagccaagga gagcattgac
     9181 agcctcgaga gcgccaaccc caagctgtat gccaattaca tcaaatacag cgacgagaag
     9241 aaagccgagg agttcaccag gcagattaac agggagaagg ccaaaaccgc cctgaacgcc
     9301 tacctgagga acaccaagtg gaatgtgatc atcagggagg acctcctgag aattgacaac
     9361 aagacatgta ccctgttcag aaacaaggcc gtccacctgg aagtggccag gtatgtccac
     9421 gcctatatca acgacattgc cgaggtcaat tcctacttcc aactgtacca ttacatcatg
     9481 cagagaatta tcatgaatga gaggtacgag aaaagcagcg gaaaggtgtc cgagtacttc
     9541 gacgctgtga atgacgagaa gaagtacaac gataggctcc tgaaactgct gtgtgtgcct
     9601 ttcggctact gtatccccag gtttaagaac ctgagcatcg aggccctgtt cgataggaac
     9661 gaggccgcca agttcgacaa ggagaaaaag aaggtgtccg gcaattccgg atccggacct
     9721 aagaaaaaga ggaaggtggc ggccgcttac ccatacgatg ttccagatta cgctgctagc
     9781 ggcagtggag gcggatctgg aggtagtggt ggatctg
//
