LOCUS       sequence-36476-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET MBP mOrange LIC cloning vector (MBP-mOrange)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(63..770)
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(341..360)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(807..827)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcttac
       61 tattacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
      121 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gccgacgatg
      181 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcggaggtg
      241 tagtggccgc cgtccttcag cttcagcctc atcttgatct cgcccttcag ggcgccgtcc
      301 tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg
      361 ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgg
      421 agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc
      481 cacttgaagc cctcggggaa ggacagcttg aagtagtcgg ggatgtcggc ggggtgcttc
      541 acgtaggcct tggagccgta ggtgaactga ggggacagga tgtcccaggc gaagggcagg
      601 gggccaccct tggtcacctt cagcttagcg gtctgaaagc cctcgtaggg gcggccctcg
      661 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgcg caccttgaag
      721 cgcatgaact ccttgatgat ggccatgtta ttctcctcgc ccttgctcac ggatccgctg
      781 ctgccgttgc tcccactacc aatattggat tggaagtaca ggttttcctc gatcccgag
//
