LOCUS       sequence-364794-        1906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1
            (seperated by P2A and T2A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Igelmann S, Lessard F, Uchenunu O, Bouchard J, Fernandez-Ruiz A, 
            Rowell MC, Lopes-Paciencia S, Papadopoli D, Fouillen A, Ponce KJ, 
            Huot G, Mignacca L, Benfdil M, Kalegari P, Wahba HM, Pencik J, Vuong
            N, Quenneville J, Guillon J, Bourdeau V, Hulea L, Gagnon E, Kenner 
            L, Moriggl R, Nanci A, Pollak MN, Omichinski JG, Topisirovic I, 
            Ferbeyre G
  TITLE     A hydride transfer complex reprograms NAD metabolism and bypasses 
            senescence.
  JOURNAL   Mol Cell. 2021 Sep 16;81(18):3848-3865.e19. doi: 
            10.1016/j.molcel.2021.08.028.
  PUBMED    34547241
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.08"; journalName: "Mol Cell"; date: 
            "2021-09-16- 16"; volume: "81"; issue: "18"; pages: "3848-3865.e19"
COMMENT     Sequence Label: pUltra-MDH1-ME1
FEATURES             Location/Qualifiers
     source          1..1906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(200..219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gap             384..420
                     /estimated_length=37
     CDS             complement(join(879..1670,1671..1739))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARXGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1502..1521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1740..1844)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
       61 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tgagaggtgg
      181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      361 aagctgggct gtgtgcacga mysnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 katcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      541 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      721 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      781 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      841 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
      901 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      961 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1021 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1081 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1141 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1201 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1261 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1321 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1381 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1441 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     1501 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     1561 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccankcgt
     1621 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1681 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     1741 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     1801 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1861 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgtt
//
